RFC3 (NM_002915) Human Untagged Clone

CAT#: SC324250

RFC3 (untagged)-Human replication factor C (activator 1) 3, 38kDa (RFC3), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RFC3
• Synonyms: RFC38
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_002915.3 GCGGGATTTTCAAGCGTAGGCCCCCGGGAACTCGAGCTGCCATGAGCCTCTGGGTGGACA
  AGTATCGGCCCTGCTCCTTGGGACGGCTGGACTATCACAAGGAGCAGGCGGCCCAGCTGC
  GGAACCTGGTGCAGTGTGGTGACTTTCCTCATCTGTTAGTGTACGGACCATCAGGTGCTG
  GAAAAAAGACAAGAATTATGTGTATTCTACGTGAACTTTATGGTGTTGGAGTGGAAAAAT
  TGAGAATTGAACATCAGACCATCACAACTCCATCTAAAAAAAAAATTGAAATTAGCACCA
  TTGCAAGTAACTACCACCTTGAAGTTAATCCTAGTGATGCTGGAAATAGTGACCGAGTAG
  TCATTCAGGAGATGTTGAAAACAGTGGCACAATCACAACAACTTGAAACAAACTCTCAAA
  GGGATTTTAAAGTGGTATTATTGACAGAAGTTGACAAACTCACCAAAGATGCTCAGCATG
  CCTTGCGAAGAACCATGGAAAAATATATGTCTACCTGCAGATTGATCTTGTGCTGCAATT
  CTACATCTAAAGTGATCCCACCTATTCGTAGTAGGTGCTTGGCGGTTCGTGTGCCTGCTC
  CCAGCATTGAAGATATTTGCCACGTGTTATCTACTGTGTGTAAGAAGGAAGGTCTGAATC
  TTCCTTCACAACTGGCTCATAGACTTGCAGAGAAGTCTTGTAGAAATCTCAGAAAAGCCC
  TGCTTATGTGTGAAGCCTGCAGAGTGCAACAATATCCTTTTACTGCAGATCAAGAAATCC
  CTGAGACAGATTGGGAGGTGTATCTGAGGGAGACTGCAAATGCTATTGTCAGTCAGCAAA
  CTCCACAAAGGCTCCTTGAAGTTCGTGGAAGGCTGTATGAGCTTCTAACTCATTGTATTC
  CTCCTGAGATAATAATGAAGGGCCTTCTCTCAGAACTGTTACATAATTGTGATGGACAAC
  TGAAAGGGGAGGTGGCACAAATGGCAGCTTACTATGAGCATCGTCTACAGCTGGGTAGCA
  AAGCCATTTATCACTTGGAAGCGTTTGTGGCCAAATTCATGGCACTTTATAAGAAGTTCA
  TGGAGGATGGATTGGAAGGCATGATGTTCTGACTTCTGTCAGTTATTCTTGCAAAGATTT
  CTCAGTATCAGTATTTACATACAGCTTATATTAAAAGAGCTGTGGGTAAATTAACTGAAC
  TTAATCATGTCGTATTTGCGTTTTTTTGGTAATAACTTCTCTGTGAACTATTAATCATCC
  TCTGAGTTAAATAATTGCTCCTATACTATTGAAGTATGTAGTTTTGTACATAACTTAGAG
  ACTTTAGAGTCTAAGAAAATGATCTTAATTTACTTTAAGCATTGGTTATTCAAGTATTCA
  TTGTTGATCCTCCTATTCTCTTCCGTCTAATCTCTCACCTGCTAAAGGAGATTTACACAT
  TAGAAAGCAAAGATTATTTTCATTTATCCAGATGACCATTTTCTGCCACAGGTAACATGA
  TTGTTTGACACACCATTATATTTAATTCTAGTTTCTCTCAATGAATAATTGTATTTTTGT
  AGGAAATGTAAGATTTCATTCTGAAACATAATTATTGGTATGGACAAAATTGCAGATACC
  ATTTCTGTTGAGGCTGCAGATTTCCAACTTTTATTTCAGTGGTTCAGATTAGTATTAGGT
  CGGTACTAAGAAATAAGCATGTTTTCACTAATTTAAGTACTTGAGACTCTTGAAGAAAAT
  TCAGAATGAAGTTCTGGAGAAAGGTATGTTACTGTAGTAATTACTCTTTGAACAGGTTTT
  GTGTTTTGTCATTAGCTCTGCCCTTTTTAATTAAATATTTTGGTTCATGGACCAAAGGGT
  TTACTTGACAAATTTGTGTGACAGACTCCGAACAATTCCTTTACTACGAAGTATAATTTA
  TAAAATAAAATATACCCATTTTAAGGGTACAGTTTGATTTTTGACCAGTGAAACTATGAT
  CCCAATCAAGGTATAGATGCCGTCACCCCAAAAAGTTCCCTCCATATCCCTTTGCAGTCA
  GTTCATCCCTACCCTGGCCCAGATGATCACTGATCTTGTCATTATAGATGAGTTTTGCCA
  GTTCAAGAATTTAATGGAATCAGATATTGTAAGCATTCTTGTGTAATACTTCATTCTCTC
  TCATTATTGAGATTCATCCATATTGTTGAATGTTTCACTAGTTAATGTTTATTGTTCAAT
  ATTTTTGTATATACTTTTAAAGCCTATTCACTTGCTGATGGATCTTTGGTTTGTTTCCAG
  GTTTGGTTATTATGAATAAAGTTGCTGTGAATAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_002915
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002915.3, NP_002906.1
• RefSeq Size: 2396 bp
• RefSeq ORF: 1071 bp
• Locus ID: 5983
• UniProt ID: P40938
• Cytogenetics: 13q13.2
• Domains: AAA
• Protein Families: Stem cell - Pluripotency
• Protein Pathways: DNA replication, Mismatch repair, Nucleotide excision repair
• Gene Summary: The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kDa. This gene encodes the 38 kDa subunit. This subunit is essential for the interaction between the 140 kDa subunit and the core complex that consists of the 36, 37, and 40 kDa subunits. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) encodes the longer isoform (1).