DCK (NM_000788) Human Untagged Clone

CAT#: SC324233

DCK (untagged)-Human deoxycytidine kinase (DCK)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DCK
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000788.1 CGCAGGCCCGCCAGTGTCCTCAGCTGCCTCCGCGCGCCAAAGTCAAACCCCGACACCCGC
  CGGCGGGCCGGTGAGCTCACTAGCTGACCCGGCAGGTCAGGATCTGGCTTAGCGGCGCCG
  CGAGCTCCAGTGCGCGCACCCGTGGCCGCCTCCCAGCCCTCTTTGCCGGACGAGCTCTGG
  GCCGCCACAAGACTAAGGAATGGCCACCCCGCCCAAGAGAAGCTGCCCGTCTTTCTCAGC
  CAGCTCTGAGGGGACCCGCATCAAGAAAATCTCCATCGAAGGGAACATCGCTGCAGGGAA
  GTCAACATTTGTGAATATCCTTAAACAATTGTGTGAAGATTGGGAAGTGGTTCCTGAACC
  TGTTGCCAGATGGTGCAATGTTCAAAGTACTCAAGATGAATTTGAGGAACTTACAATGTC
  TCAGAAAAATGGTGGGAATGTTCTTCAGATGATGTATGAGAAACCTGAACGATGGTCTTT
  TACCTTCCAAACATATGCTTGTCTCAGTCGAATAAGAGCTCAGCTTGCCTCTCTGAATGG
  CAAGCTCAAAGATGCAGAGAAACCTGTATTATTTTTTGAACGATCTGTGTATAGTGACAG
  GTATATTTTTGCATCTAATTTGTATGAATCTGAATGCATGAATGAGACAGAGTGGACAAT
  TTATCAAGACTGGCATGACTGGATGAATAACCAATTTGGCCAAAGCCTTGAATTGGATGG
  AATCATTTATCTTCAAGCCACTCCAGAGACATGCTTACATAGAATATATTTACGGGGAAG
  AAATGAAGAGCAAGGCATTCCTCTTGAATATTTAGAGAAGCTTCATTATAAACATGAAAG
  CTGGCTCCTGCATAGGACACTGAAAACCAACTTCGATTATCTTCAAGAGGTGCCTATCTT
  AACACTGGATGTTAATGAAGACTTTAAAGACAAATATGAAAGTCTGGTTGAAAAGGTCAA
  AGAGTTTTTGAGTACTTTGTGATCTTGCTGAAGACTACAGGCAGCCAAATGGTTCCAGAT
  ACTTCAGCTTTGTGTATCTTCGTAACTTCATATTAATATAAGTTTCTTTAGAAAACCCAA
  GTTTTTAATCGTTTTTGTTTTAAGGAAAAAAGATTTTTAAAATGAATCTTATGCAAAACT
  TTTTGATCAGTTTCTTTTCTTTTGTTTTTTTTTTAAAAAAGACATTTAAAGACAAAGACA
  TTATTTCTCATAGCAGGAAATGTAGAGGTAGATGGTTCCAGTATCAGCATAGTGACTAAA
  CTACATTATAAAAGATCCAGCTTCCTTCTGTCATTCCCCTCTTTTGTCTTCCTCAGCAGG
  TTGGCTTTTTTCCCTGGTGCCTCTCACTTCGTTGGTGACCAGTTTCTTAAACTGAAAGCT
  TTAATGTTACATAGTAAATGGTAGTGTGTCCTGTGTAAATTAGTGTACCTATTAAAAGTT
  GCAAAGTGGAATTAAAGGAATCCCTAGAATAAGGATTCTGAAGTTTTATTTTAAATTATT
  ATCTTCTTAACAGTTTAGTCCCACCTCTTACTTCCTGCCTCAGTCTGCTTTCTCTACTGT
  CTGGATTAATTAGGCAGCCTGCTATAAAGTTAAAGTCACACATTTCTATTTTGCAAACAC
  TGTGATTACTCTTTGCTTTGTAGTTTGCTTTGCTTTGTAGGGTTCTGCTTTTAAGTTTTT
  CTCTTTTTCAGACAAATTACTGATAAAAATGATATTGCTCTATATGTAATATATCCTGAA
  AGCATTATTTTTTGTTGAATAGGAAATAAAATTAATGAAGACAGAGGCTAGAAAGCATCC
  ATTAATTAATGAGACACACTTAACTACTTATCTCTAAACCATCTATGTGAATATTTGTAA
  AAATAATGAATGGACTCATCTTAGTTCTGTATATAAATATATTTTCTTTCTAGTTTGTTT
  AGTTAAGGTGTGCAGTGTTTTTCCTGTGTATTAAACCTTTCCATTTTACGTTTTAGAAAA
  TTTTATGTATTTTAAAATAAGGGGAAGAGTCATTTTCACTTTTAAACTACTATTTTTCTT
  TCCAAGTCATTTTTGTTTTTGGTTTCTTATTCAAAGATGATAATTTAGTGGATTAACCAG
  TCCAGACGCACTGATCTTTGCAAAGGAGACTTAATTTCAAATCTGTAATTACCATACATA
  AACTGTCTCATTATAAGTATGCATTTTTTTAGTTTGTTTTTGTTTGGTATAAATTAATTT
  GTTAATTAAATATTTCTTAAGTATAAACCTTATGAACTACAGTGGAGCTACACTCATTGA
  AATGTAATTTCAGTTCTAAAAAGATGTAATAATCATTTTAGAATTAAAATTTATTCTACT
  TTTAAATAAATTATGAATATTAAAGGTGAAAATTGTATAAATTACTTTGATTCCATTTTA
  AGTGGAGACATATTTCAGTGATTTTTAGTAACCTTTAAAAATGTATAATGACTTTTAAAA
  TTTGTAGAATTGAAAAGACGCTAATAAAAATTTATTATTTATTTGTCATGACTCAAAAAA
  AAAAAAAAA
• Restriction Sites: ECoRI-NOT
• ACCN: NM_000788
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000788.1, NP_000779.1
• RefSeq Size: 2460 bp
• RefSeq ORF: 783 bp
• Locus ID: 1633
• UniProt ID: P27707
• Cytogenetics: 4q13.3
• Domains: dNK
• Protein Families: Druggable Genome
• Protein Pathways: Purine metabolism, Pyrimidine metabolism
• Gene Summary: Deoxycytidine kinase (DCK) is required for the phosphorylation of several deoxyribonucleosides and their nucleoside analogs. Deficiency of DCK is associated with resistance to antiviral and anticancer chemotherapeutic agents. Conversely, increased deoxycytidine kinase activity is associated with increased activation of these compounds to cytotoxic nucleoside triphosphate derivatives. DCK is clinically important because of its relationship to drug resistance and sensitivity. [provided by RefSeq, Jul 2008]