PYK2 (PTK2B) (NM_004103) Human Untagged Clone

CAT#: SC324205

PTK2B (untagged)-Human PTK2B protein tyrosine kinase 2 beta (PTK2B), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PYK2
• Synonyms: CADTK; CAKB; FADK2; FAK2; PKB; PTK; PYK2; RAFTK
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_004103.3 TCGATCTAACCTGTCAGCCCTTTTACTCAGCCACAGCCTCCGGAGCCGTTGCACACCTAC
  CTGCCCGGCCGACTTACCTGTACTTGCCGCCGTCCCGGCTCACCTGGCGGTGCCCGAGGA
  GTAGTCGCTGGAGTCCGCGCCTCCCTGGGACTGCAATGTGCCGATCTTAGCTGCTGCCTG
  AGAGGATGTCTGGGGTGTCCGAGCCCCTGAGTCGAGTAAAGTTGGGCACGTTACGCCGGC
  CTGAAGGCCCTGCAGAGCCCATGGTGGTGGTACCAGTAGATGTGGAAAAGGAGGACGTGC
  GTATCCTCAAGGTCTGCTTCTATAGCAACAGCTTCAATCCTGGGAAAAACTTCAAACTGG
  TCAAATGCACTGTCCAGACGGAGATCCGGGAGATCATCACCTCCATCCTGCTGAGCGGGC
  GGATCGGGCCCAACATCCGGTTGGCTGAGTGCTATGGGCTGAGGCTGAAGCACATGAAGT
  CCGATGAGATCCACTGGCTGCACCCACAGATGACAGTGGGTGAGGTGCAGGACAAGTATG
  AGTGTCTGCACGTGGAAGCCGAGTGGAGGTATGACCTTCAAATCCGCTACTTGCCAGAAG
  ACTTCATGGAGAGCCTGAAGGAGGACAGGACCACGCTGCTCTATTTTTACCAACAGCTCC
  GGAACGACTACATGCAGCGCTACGCCAGCAAGGTCAGCGAGGGCATGGCCCTGCAGCTGG
  GCTGCCTGGAGCTCAGGCGGTTCTTCAAGGATATGCCCCACAATGCACTTGACAAGAAGT
  CCAACTTCGAGCTCCTAGAAAAGGAAGTGGGGCTGGACTTGTTTTTCCCAAAGCAGATGC
  AGGAGAACTTAAAGCCCAAACAGTTCCGGAAGATGATCCAGCAGACCTTCCAGCAGTACG
  CCTCGCTCAGGGAGGAGGAGTGCGTCATGAAGTTCTTCAACACTCTCGCCGGCTTCGCCA
  ACATCGACCAGGAGACCTACCGCTGTGAACTCATTCAAGGATGGAACATTACTGTGGACC
  TGGTCATTGGCCCTAAAGGGATCCGCCAGCTGACTAGTCAGGACGCAAAGCCCACCTGCC
  TGGCCGAGTTCAAGCAGATCAGGTCCATCAGGTGCCTCCCGCTGGAGGAGGGCCAGGCAG
  TACTTCAGCTGGGCATTGAAGGTGCCCCCCAGGCCTTGTCCATCAAAACCTCATCCCTAG
  CAGAGGCTGAGAACATGGCTGACCTCATAGACGGCTACTGCCGGCTGCAGGGTGAGCACC
  AAGGCTCTCTCATCATCCATCCTAGGAAAGATGGTGAGAAGCGGAACAGCCTGCCCCAGA
  TCCCCATGCTAAACCTGGAGGCCCGGCGGTCCCACCTCTCAGAGAGCTGCAGCATAGAGT
  CAGACATCTACGCAGAGATTCCCGACGAAACCCTGCGAAGGCCCGGAGGTCCACAGTATG
  GCATTGCCCGTGAAGATGTGGTCCTGAATCGTATTCTTGGGGAAGGCTTTTTTGGGGAGG
  TCTATGAAGGTGTCTACACAAATCATAAAGGGGAGAAAATCAATGTAGCTGTCAAGACCT
  GCAAGAAAGACTGCACTCTGGACAACAAGGAGAAGTTCATGAGCGAGGCAGTGATCATGA
  AGAACCTCGACCACCCGCACATCGTGAAGCTGATCGGCATCATTGAAGAGGAGCCCACCT
  GGATCATCATGGAATTGTATCCCTATGGGGAGCTGGGCCACTACCTGGAGCGGAACAAGA
  ACTCCCTGAAGGTGCTCACCCTCGTGCTGTACTCACTGCAGATATGCAAAGCCATGGCCT
  ACCTGGAGAGCATCAACTGCGTGCACAGGGACATTGCTGTCCGGAACATCCTGGTGGCCT
  CCCCTGAGTGTGTGAAGCTGGGGGACTTTGGTCTTTCCCGGTACATTGAGGACGAGGACT
  ATTACAAAGCCTCTGTGACTCGTCTCCCCATCAAATGGATGTCCCCAGAGTCCATTAACT
  TCCGACGCTTCACGACAGCCAGTGACGTCTGGATGTTCGCCGTGTGCATGTGGGAGATCC
  TGAGCTTTGGGAAGCAGCCCTTCTTCTGGCTGGAGAACAAGGATGTCATCGGGGTGCTGG
  AGAAAGGAGACCGGCTGCCCAAGCCTGATCTCTGTCCACCGGTCCTTTATACCCTCATGA
  CCCGCTGCTGGGACTACGACCCCAGTGACCGGCCCCGCTTCACCGAGCTGGTGTGCAGCC
  TCAGTGACGTTTATCAGATGGAGAAGGACATTGCCATGGAGCAAGAGAGGAATGCTCGCT
  ACCGAACCCCCAAAATCTTGGAGCCCACAGCCTTCCAGGAACCCCCACCCAAGCCCAGCC
  GACCTAAGTACAGACCCCCTCCGCAAACCAACCTCCTGGCTCCAAAGCTGCAGTTCCAGG
  TTCCTGAGGGTCTGTGTGCCAGCTCTCCTACGCTCACCAGCCCTATGGAGTATCCATCTC
  CCGTTAACTCACTGCACACCCCACCTCTCCACCGGCACAATGTCTTCAAACGCCACAGCA
  TGCGGGAGGAGGACTTCATCCAACCCAGCAGCCGAGAAGAGGCCCAGCAGCTGTGGGAGG
  CTGAAAAGGTCAAAATGCGGCAAATCCTGGACAAACAGCAGAAGCAGATGGTGGAGGACT
  ACCAGTGGCTCAGGCAGGAGGAGAAGTCCCTGGACCCCATGGTTTATATGAATGATAAGT
  CCCCATTGACGCCAGAGAAGGAGGTCGGCTACCTGGAGTTCACAGGGCCCCCACAGAAGC
  CCCCGAGGCTGGGCGCACAGTCCATCCAGCCCACAGCTAACCTGGACCGGACCGATGACC
  TGGTGTACCTCAATGTCATGGAGCTGGTGCGGGCCGTGCTGGAGCTCAAGAATGAGCTCT
  GTCAGCTGCCCCCCGAGGGCTACGTGGTGGTGGTGAAGAATGTGGGGCTGACCCTGCGGA
  AGCTCATCGGGAGCGTGGATGATCTCCTGCCTTCCTTGCCGTCATCTTCACGGACAGAGA
  TCGAGGGCACCCAGAAACTGCTCAACAAAGACCTGGCAGAGCTCATCAACAAGATGCGGC
  TGGCGCAGCAGAACGCCGTGACCTCCCTGAGTGAGGAGTGCAAGAGGCAGATGCTGACGG
  CTTCACACACCCTGGCTGTGGACGCCAAGAACCTGCTCGACGCTGTGGACCAGGCCAAGG
  TTCTGGCCAATCTGGCCCACCCACCTGCAGAGTGACGGAGGGTGGGGGCCACCTGCCTGC
  GTCTTCCGCCCCTGCCTGCCATGTACCTCCCCTGCCTTGCTGTTGGTCATGTGGGTCTTC
  CAGGGAGAAGGCCAAGGGGAGTCACCTTCCCTTGCCACTTTGCACGACGCCCTCTCCCCA
  CCCCTACCCCTGGCTGTACTGCTCAGGCTGCAGCTGGACAGAGGGGACTCTGGGCTATGG
  ACACAGGGTGACGGTGACAAAGATGGCTCAGAGGGGGACTGCTGCTGCCTGGCCACTGCT
  CCCTAAGCCAGCCTGGTCCATGCAGGGGGCTCCTGGGGGTGGGGAGGTGTCACATGGTGC
  CCCTAGCTTTATATATGGACATGGCAGGCCGATTTGGGAACCAAGCTATTCCTTTCCCTT
  CCTCTTCGGCCCTCAGATGTCCCTTGATGCACAGAGAAGCTGGGGAGGAGCTTTGTTTTG
  GGGGTCAGGCAGCCAGTGAGATGAGGGATGGGCCTGGCATTCTTGTACAGTGTATATTGA
  AATTTATTTAATGTGAGTTTGGTCTGGACTGACAGCATGTGCCCTCCTGAGGGAGGACCT
  GGGGCACAGTCCAGGAACAAGCTAATTGGGAGTCCAGGCACAGGATGCTGTGTTGTCAAC
  AAACCAAGCATCAGGGGGAAGAAGCAGAGAGATGCGGCCAAGATAGGACCTTGGGCCAAA
  TCCGCTCTCTTCCTGCCCCTCTTTCTCTTTCTTCCTTTACTTTCCCTTGCTTTTCCCTCT
  TTTCTTACTCCTCCTCTTTCTCTCCCCAACCCCCATTCTCATCTGCACCCTTCTTTTCTC
  ATGTGTTTGCATAAACATTCTTTTAACTTCTTTCTATTTGACTTGTGGTTGAATTAAAAT
  TGTCCCATTTGCTTTGAAAAAAAAAAAAAAA
• Restriction Sites: ECoRI-NOT
• ACCN: NM_004103
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004103.3, NP_004094.3
• RefSeq Size: 4555 bp
• RefSeq ORF: 3030 bp
• Locus ID: 2185
• UniProt ID: Q14289
• Cytogenetics: 8p21.2
• Domains: B41, pkinase, TyrKc, S_TKc, Focal_AT
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Calcium signaling pathway, Chemokine signaling pathway, GnRH signaling pathway, Leukocyte transendothelial migration, Natural killer cell mediated cytotoxicity
• Gene Summary: This gene encodes a cytoplasmic protein tyrosine kinase which is involved in calcium-induced regulation of ion channels and activation of the map kinase signaling pathway. The encoded protein may represent an important signaling intermediate between neuropeptide-activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity. The encoded protein undergoes rapid tyrosine phosphorylation and activation in response to increases in the intracellular calcium concentration, nicotinic acetylcholine receptor activation, membrane depolarization, or protein kinase C activation. This protein has been shown to bind CRK-associated substrate, nephrocystin, GTPase regulator associated with FAK, and the SH2 domain of GRB2. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Four transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1, 2, and 3 all encode isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.