Thymine DNA glycosylase (TDG) (NM_003211) Human Untagged Clone

CAT#: SC324192

TDG (untagged)-Human thymine-DNA glycosylase (TDG)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Thymine DNA glycosylase
• Synonyms: hTDG
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_003211.3 GGGGGACAGTAGAAGCCTGGAGGAGGAGCTTGAGTCCAGCCACTGTCTGGGTACTGCCAG
  CCATCGGGCCCAGGTCTCTGGGGTTGTCTTACCGCAGTGAGTACCACGCGGTACTACAGA
  GACCGGCTGCCCGTGTGCCCGGCAGGTGGAGCCGCCCGCATCAGCGGCCTCGGGGAATGG
  AAGCGGAGAACGCGGGCAGCTATTCCCTTCAGCAAGCTCAAGCTTTTTATACGTTTCCAT
  TTCAACAACTGATGGCTGAAGCTCCTAATATGGCAGTTGTGAATGAACAGCAAATGCCAG
  AAGAAGTTCCAGCCCCAGCTCCTGCTCAGGAACCAGTGCAAGAGGCTCCAAAAGGAAGAA
  AAAGAAAACCCAGAACAACAGAACCAAAACAACCAGTGGAACCCAAAAAACCTGTTGAGT
  CAAAAAAATCTGGCAAGTCTGCAAAATCAAAAGAAAAACAAGAAAAAATTACAGACACAT
  TTAAAGTAAAAAGAAAAGTAGACCGTTTTAATGGTGTTTCAGAAGCTGAACTTCTGACCA
  AGACTCTCCCCGATATTTTGACCTTCAATCTGGACATTGTCATTATTGGCATAAACCCGG
  GACTAATGGCTGCTTACAAAGGGCATCATTACCCTGGACCTGGAAACCATTTTTGGAAGT
  GTTTGTTTATGTCAGGGCTCAGTGAGGTCCAGCTGAACCATATGGATGATCACACTCTAC
  CAGGGAAGTATGGTATTGGATTTACCAACATGGTGGAAAGGACCACGCCCGGCAGCAAAG
  ATCTCTCCAGTAAAGAATTTCGTGAAGGAGGACGTATTCTAGTACAGAAATTACAGAAAT
  ATCAGCCACGAATAGCAGTGTTTAATGGAAAATGTATTTATGAAATTTTTAGTAAAGAAG
  TTTTTGGAGTAAAGGTTAAGAACTTGGAATTTGGGCTTCAGCCCCATAAGATTCCAGACA
  CAGAAACTCTCTGCTATGGTATGCCATCATCCAGTGCAAGATGTGCTCAGTTTCCTCGAG
  CCCAAGACAAAGTTCATTACTACATAAAACTGAAGGACTTAAGAGATCAGTTGAAAGGCA
  TTGAACGAAATATGGACGTTCAAGAGGTGCAATATACATTTGACCTACAGCTTGCCCAAG
  AGGATGCAAAGAAGATGGCTGTTAAGGAAGAAAAATATGATCCAGGTTATGAGGCAGCAT
  ATGGTGGTGCTTACGGAGAAAATCCATGCAGCAGTGAACCTTGTGGCTTCTCTTCAAATG
  GGCTAATTGAGAGCGTGGAGTTAAGAGGAGAATCAGCTTTCAGTGGCATTCCTAATGGGC
  AGTGGATGACCCAGTCATTTACAGACCAAATTCCTTCCTTTAGTAATCACTGTGGAACAC
  AAGAACAGGAAGAAGAAAGCCATGCTTAAGAATGGTGCTTCTCAGCTCTGCTTAAATGCT
  GCAGTTTTAATGCAGTTGTCAACAAGTAGAACCTCAGTTTGCTAACTGAAGTGTTTTATT
  AGTATTTTACTCTAGTGGTGTAATTGTAATGTAGAACAGTTGTGTGGTAGTGTGAACCGT
  ATGAACCTAAGTAGTTTGGAAGAAAAAGTAGGGTTTTTGTATACTAGCTTTTGTATTTGA
  ATTAATTATCATTCCAGCTTTTTATATACTATATTTCATTTATGAAGAAATTGATTTTCT
  TTTGGGAGTCACTTTTAATCTGTAATTTTAAAATACAAGTCTGAATATTTATAGTTGATT
  CTTAACTGTGCATAAACCTAGATATACCATTATCCCTTTTATACCTAAGAAGGGCATGCT
  AATAATTACCACTGTCAAAGAGGCAAAGGTGTTGATTTTTGTATATGAAGTTAAGCCTCA
  GTGGAGTCTCATTTGTTAGTTTTTAGTGGTAACTAAGGGTAAACTCAGGGTTCCCTGAGC
  TATATGCACACTCAGACCTCTTTGCTTTACCAGTGGTGTTTGTGAGTTGCTCAGTAGTAA
  AAACTGGCCTTACCTGACAGAGCCCTGGCTTTGACCTGCTCAGCCCTGTGTGTTAATCCT
  CTAGTAGCCAATTAACTACTCTGGGGTGGCAGGTTCCAGAGAATGCAGTAGACCTTTTGC
  CACTCATCTGTGTTTTACTTGAGACATGTAAATATGATAGGGAAGGAACTGAATTTCTCC
  ATTCATATTTATAACCATTCTAGTTTTATCTTCCTTGGCTTTAAGAGTGTGCCATGGAAA
  GTGATAAGAAATGAACTTCTAGGCTAAGCAAAAAGATGCTGGAGATATTTGATACTCTCA
  TTTAAACTGGTGCTTTATGTACATGAGATGTACTAAAATAAGTAATATAGAATTTTTCTT
  GCTAGGTAAATCCAGTAAGCCAATAATTTTAAAGATTCTTTATCTGCATCATTGCTGTTT
  GTTACTATAAATTAAATGAACCTCATGGAAAGGTTGAGGTGTATACCTTTGTGATTTTCT
  AATGAGTTTTCCATGGTGCTACAAATAATCCAGACTACCAGGTCTGGTAGATATTAAAGC
  TGGGTACTAAGAAATGTTATTTGCATCCTCTCAGTTACTCCTGAATATTCTGATTTCATA
  CGTACCCAGGGAGCATGCTGTTTTGTCAATCAATATAAAATATTTATGAGGTCTCCCCCA
  CCCCCAGGAGGTTATATGATTGCTCTTCTCTTTATAATAAGAGAAACAAATTCTTATTGT
  GAATCTTAACATGCTTTTTAGCTGTGGCTATGATGGATTTTATTTTTTCCTAGGTCAAGC
  TGTGTAAAAGTCATTTATGTTATTTAAATGATGTACTGTACTGCTGTTTACATGGACGTT
  TTGTGCGGGTGCTTTGAAGTGCCTTGCATCAGGGATTAGGAGCAATTAAATTATTTTTTC
  ACGGGACTGTGTAAAGCATGTAACTAGGTATTGCTTTGGTATATAACTATTGTAGCTTTA
  CAAGAGATTGTTTTATTTGAATGGGGAAAATACCCTTTAAATTATGACGGACATCCACTA
  GAGATGGGTTTGAGGATTTTCCAAGCGTGTAATAATGATGTTTTTCCTAACATGACAGAT
  GAGTAGTAAATGTTGATATATCCTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: ECoRI-NOT
• ACCN: NM_003211
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003211.3, NP_003202.3
• RefSeq Size: 3229 bp
• RefSeq ORF: 1233 bp
• Locus ID: 6996
• UniProt ID: Q13569
• Cytogenetics: 12q23.3
• Domains: UDG
• Protein Families: Druggable Genome
• Protein Pathways: Base excision repair
• Gene Summary: The protein encoded by this gene belongs to the TDG/mug DNA glycosylase family. Thymine-DNA glycosylase (TDG) removes thymine moieties from G/T mismatches by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of DNA and the mispaired thymine. With lower activity, this enzyme also removes thymine from C/T and T/T mispairings. TDG can also remove uracil and 5-bromouracil from mispairings with guanine. This enzyme plays a central role in cellular defense against genetic mutation caused by the spontaneous deamination of 5-methylcytosine and cytosine. This gene may have a pseudogene in the p arm of chromosome 12. [provided by RefSeq, Jul 2008]