ALOX15 (NM_001140) Human Untagged Clone

CAT#: SC324183

ALOX15 (untagged)-Human arachidonate 15-lipoxygenase (ALOX15)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ALOX15
• Synonyms: 12-LOX; 15-LOX; 15-LOX-1; LOG15
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001140.3 CATCTTTGAGCAAGATGGGTCTCTACCGCATCCGCGTGTCCACTGGGGCCTCGCTCTATG
  CCGGTTCCAACAACCAGGTGCAGCTGTGGCTGGTCGGCCAGCACGGGGAGGCGGCGCTCG
  GGAAGCGACTGTGGCCCGCACGGGGCAAGGAGACAGAACTCAAGGTGGAAGTACCGGAGT
  ATCTGGGGCCGCTGCTGTTTGTGAAACTGCGCAAACGGCACCTCCTTAAGGACGACGCCT
  GGTTCTGCAACTGGATCTCTGTGCAGGGCCCCGGAGCCGGGGACGAGGTCAGGTTCCCTT
  GTTACCGCTGGGTGGAGGGCAACGGCGTCCTGAGCCTGCCTGAAGGCACCGGCCGCACTG
  TGGGCGAGGACCCTCAGGGCCTGTTCCAGAAACACCGGGAAGAAGAGCTGGAAGAGAGAA
  GGAAGTTGTACCGGTGGGGAAACTGGAAGGACGGGTTAATTCTGAATATGGCTGGGGCCA
  AACTATATGACCTCCCTGTGGATGAGCGATTTCTGGAAGACAAGAGAGTTGACTTTGAGG
  TTTCGCTGGCCAAGGGGCTGGCCGACCTCGCTATCAAAGACTCTCTAAATGTTCTGACTT
  GCTGGAAGGATCTAGATGACTTCAACCGGATTTTCTGGTGTGGTCAGAGCAAGCTGGCTG
  AGCGCGTGCGGGACTCCTGGAAGGAAGATGCCTTATTTGGGTACCAGTTTCTTAATGGCG
  CCAACCCCGTGGTGCTGAGGCGCTCTGCTCACCTTCCTGCTCGCCTAGTGTTCCCTCCAG
  GCATGGAGGAACTGCAGGCCCAGCTGGAGAAGGAGCTGGAGGGAGGCACACTGTTCGAAG
  CTGACTTCTCCCTGCTGGATGGGATCAAGGCCAACGTCATTCTCTGTAGCCAGCAGCACC
  TGGCTGCCCCTCTAGTCATGCTGAAATTGCAGCCTGATGGGAAACTCTTGCCCATGGTCA
  TCCAGCTCCAGCTGCCCCGCACAGGATCCCCACCACCTCCCCTTTTCTTGCCTACGGATC
  CCCCAATGGCCTGGCTTCTGGCCAAATGCTGGGTGCGCAGCTCTGACTTCCAGCTCCATG
  AGCTGCAGTCTCATCTTCTGAGGGGACACTTGATGGCTGAGGTCATTGTTGTGGCCACCA
  TGAGGTGCCTGCCGTCGATACATCCTATCTTCAAGCTTATAATTCCCCACCTGCGATACA
  CCCTGGAAATTAACGTCCGGGCCAGGACTGGGCTGGTCTCTGACATGGGAATTTTCGACC
  AGATAATGAGCACTGGTGGGGGAGGCCACGTGCAGCTGCTCAAGCAAGCTGGAGCCTTCC
  TAACCTACAGCTCCTTCTGTCCCCCTGATGACTTGGCCGACCGGGGGCTCCTGGGAGTGA
  AGTCTTCCTTCTATCCCCAAGATGCGCTGCGGCTCTGGGAAATCATCTATCGGTATGTGG
  AAGGAATCGTGAGTCTCCACTATAAGACAGACGTGGCTGTGAAAGACGACCCAGAGCTGC
  AGACCTGGTGTCGAGAGATCACTGAAATCGGGCTGCAAGGGGCCCAGGACCGAGGGTTTC
  CTGTCTCTTTACAGGCTCGGGACCAGGTTTGCCACTTTGTCACCATGTGTATCTTCACCT
  GCACCGGCCAACACGCCTCTGTGCACCTGGGCCAGCTGGACTGGTACTCTTGGGTGCCTA
  ATGCACCCTGCACGATGCGGCTGCCCCCGCCAACCACCAAGGATGCAACGCTGGAGACAG
  TGATGGCGACACTGCCCAACTTCCACCAGGCTTCTCTCCAGATGTCCATCACTTGGCAGC
  TGGGCAGACGCCAGCCCGTTATGGTGGCTGTGGGCCAGCATGAGGAGGAGTATTTTTCGG
  GCCCTGAGCCTAAGGCTGTGCTGAAGAAGTTCAGGGAGGAGCTGGCTGCCCTGGATAAGG
  AAATTGAGATCCGGAATGCAAAGCTGGACATGCCCTACGAGTACCTGCGGCCCAGCGTGG
  TGGAAAACAGTGTGGCCATCTAAGCGTCGCCACCCTTTGGTTATTTCAGCCCCCATCACC
  CAAGCCACAAGCTGACCCCTTCGTGGTTATAGCCCTGCCCTCCCAAGTCCCACCCTCTTC
  CCATGTCCCACCCTCCCTAGAGGGGCACCTTTTCATGGTCTCTGCACCCAGTGAACACAT
  TTTACTCTAGAGGCATCACCTGGGACCTTACTCCTCTTTCCTTCCTTCCTCCTTTCCTAT
  CTTCCTTCCTCTCTCTCTTCCTCTTTCTTCATTCAGATCTATATGGCAAATAGCCACAAT
  TATATAAATCATTTCAAGACTAGAATAGGGGGATATAATACATATTACTCCACACCTTTT
  ATGAATCAAATATGATTTTTTTGTTGTTGTTAAGACAGAGTCTCACTTTGACACCCAGGC
  TGGAGTGCAGTGGTGCCATCACCACGGCTCACTGCAGCCTCAGCGTCCTGGGCTCAAATG
  ATCCTCCCACCTCAGCCTCCTGAGTAGCTGGGACTACAGGCTCATGCCATCATGCCCAGC
  TAATATTTTTTTATTTTCGTGGAGACGGGGCCTCACTATGTTGCCTAGGCTGGAAATAGG
  ATTTTGAACCCAAATTGAGTTTAACAATAATAAAAAGTTGTTTTACGCTAAAGATGGAAA
  AGAACTAGGACTGAACTATTTTAAATAAAATATTGGCAAAAGAAAAAAAAAAAAAAAAAA
  AAAAAAA
• Restriction Sites: ECoRI-NOT
• ACCN: NM_001140
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001140.3, NP_001131.3
• RefSeq Size: 2707 bp
• RefSeq ORF: 1989 bp
• Locus ID: 246
• UniProt ID: P16050
• Cytogenetics: 17p13.2
• Protein Families: Druggable Genome
• Protein Pathways: Arachidonic acid metabolism, Linoleic acid metabolism, Metabolic pathways
• Gene Summary: This gene encodes a member of the lipoxygenase family of proteins. The encoded enzyme acts on various polyunsaturated fatty acid substrates to generate various bioactive lipid mediators such as eicosanoids, hepoxilins, lipoxins, and other molecules. The encoded enzyme and its reaction products have been shown to regulate inflammation and immunity. Multiple pseudogenes of this gene have been identified in the human genome. [provided by RefSeq, Aug 2017]