RbAp48 (RBBP4) (NM_005610) Human Untagged Clone

CAT#: SC324030

RBBP4 (untagged)-Human retinoblastoma binding protein 4 (RBBP4), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RbAp48
• Synonyms: lin-53; NURF55; RBAP48
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_005610.1 GCGAGCTCTTGCAGCCTCCCCGCCCCTCCCGCAACGCTCGACCCCAGGATTCCCCCGGCT
  CGCCTGCCCGCCATGGCCGACAAGGAAGCAGCCTTCGACGACGCAGTGGAAGAACGAGTG
  ATCAACGAGGAATACAAAATATGGAAAAAGAACACCCCTTTTCTTTATGATTTGGTGATG
  ACCCATGCTCTGGAGTGGCCCAGCCTAACTGCCCAGTGGCTTCCAGATGTAACCAGACCA
  GAAGGGAAAGATTTCAGCATTCATCGACTTGTCCTGGGGACACACACATCGGATGAACAA
  AACCATCTTGTTATAGCCAGTGTGCAGCTCCCTAATGATGATGCTCAGTTTGATGCGTCA
  CACTACGACAGTGAGAAAGGAGAATTTGGAGGTTTTGGTTCAGTTAGTGGAAAAATTGAA
  ATAGAAATCAAGATCAACCATGAAGGAGAAGTAAACAGGGCCCGTTATATGCCCCAGAAC
  CCTTGTATCATCGCAACAAAGACTCCTTCCAGTGATGTTCTTGTCTTTGACTATACAAAA
  CATCCTTCTAAACCAGATCCTTCTGGAGAGTGCAACCCAGACTTGCGTCTCCGTGGACAT
  CAGAAGGAAGGCTATGGGCTTTCTTGGAACCCAAATCTCAGTGGGCACTTACTTAGTGCT
  TCAGATGACCATACCATCTGCCTGTGGGACATCAGTGCCGTTCCAAAGGAGGGAAAAGTG
  GTAGATGCGAAGACCATCTTTACAGGGCATACGGCAGTAGTAGAAGATGTTTCCTGGCAT
  CTACTCCATGAGTCTCTGTTTGGGTCAGTTGCTGATGATCAGAAACTTATGATTTGGGAT
  ACTCGTTCAAACAATACTTCCAAACCAAGCCACTCAGTTGATGCTCACACTGCTGAAGTG
  AACTGCCTTTCTTTCAATCCTTATAGTGAGTTCATTCTTGCCACAGGATCAGCTGACAAG
  ACTGTTGCCTTGTGGGATCTGAGAAATCTGAAACTTAAGTTGCATTCCTTTGAGTCACAT
  AAGGATGAAATATTCCAGGTTCAGTGGTCACCTCACAATGAGACTATTTTAGCTTCCAGT
  GGTACTGATCGCAGACTGAATGTCTGGGATTTAAGTAAAATTGGAGAGGAACAATCCCCA
  GAAGATGCAGAAGACGGGCCACCAGAGTTGTTGTTTATTCATGGTGGTCATACTGCCAAG
  ATATCTGATTTCTCCTGGAATCCCAATGAACCTTGGGTGATTTGTTCTGTATCAGAAGAC
  AATATCATGCAAGTGTGGCAAATGGCAGAGAACATTTATAATGATGAAGACCCTGAAGGA
  AGCGTGGATCCAGAAGGACAAGGGTCCTAGATATGTCTTTACTTGTTGTGATTTTAGACT
  CCCCTTTTTTCTTCTCAACCCTGAGAGTGATTTAACACTGGTTTTGAGACAGACTTTATT
  CAGCTATCCCTCTATATAATAGGTACCACCGATAATGCTATTAGCCCAAACCGTGGGTGT
  TTTCTAAATATTAATAGGGGGGCTTGATTCAACAAAGCCACAGACTTAACGTTGAAATTT
  TCTTCAGGAATTTTCTAGTAACCCAGGTCTAAAGTAGCTACAGAAAGGGGAATATTATGT
  GTGATTATTTTTCTTCTTATGCTATATCCCCAAGTTTTTCAGACTCATTTAAGTAAAGGC
  TAGAGTGAGTAAGGAATAGAGCCAAATGAGGTAGGTGTCTGAGCCATGAAGTATAAATAC
  TGAAAGATGTCACTTTTATTCAGGAAATAGGGGGAGATTCAAGTCGTATAGATTCCTACT
  CGAAAATCTTGACACCTGACTTTCCAGGATGCACATTTTCATACGTAGACCAGTTTCCTC
  TTGGTTTCTTCAGTTAAGTCAAAACAACACGTTCCTCTTTCCCCATATATTCATATATTT
  TTGCTCGTTAGTGTATTTCTTGAGCTGTTTTCATGTTGTTTATTTCCTGTCTGTGAAATG
  GTGTTTTTTTTTTTGTTGTTGGTTTTTTTTTTTTTTTTTAACTTGGGACCACCAAGTTGT
  AAAGATGTATGTTTTTACCTGACAGTTATACCACAGGTAGACTGTCAAGTTGAGAAGAGT
  GAATCAATAACTTGTATTTGTTTTAAAAATTAAATTAATCCTTGATAAGAGTTGCTTTTT
  TTTTTAGGAGTTAGTCCTTGACCACTAGTTTGATGCCATCTCCATTTTGGGTGACCTGTT
  TCACCAGCAGGCCTGTTACTCTCCATGACTAACTGTGTAAGTGCTTAAAATGGAATAAAT
  TGCTTTTCTACATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: ECoRI-NOT
• ACCN: NM_005610
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005610.1, NP_005601.1
• RefSeq Size: 2314 bp
• RefSeq ORF: 1278 bp
• Locus ID: 5928
• UniProt ID: Q09028
• Cytogenetics: 1p35.1
• Domains: WD40
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: This gene encodes a ubiquitously expressed nuclear protein which belongs to a highly conserved subfamily of WD-repeat proteins. It is present in protein complexes involved in histone acetylation and chromatin assembly. It is part of the Mi-2 complex which has been implicated in chromatin remodeling and transcriptional repression associated with histone deacetylation. This encoded protein is also part of co-repressor complexes, which is an integral component of transcriptional silencing. It is found among several cellular proteins that bind directly to retinoblastoma protein to regulate cell proliferation. This protein also seems to be involved in transcriptional repression of E2F-responsive genes. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2008]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments.