INPP5E (NM_019892) Human Untagged Clone

CAT#: SC323870

INPP5E (untagged)-Human inositol polyphosphate-5-phosphatase, 72 kDa (INPP5E)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: INPP5E
• Synonyms: CORS1; CPD4; JBTS1; MORMS; pharbin; PPI5PIV
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_019892.3 CTGGGCGGCCGGGCCTCCGCGAGTACCGGGTCGACTTCGGAGTCTGCGAGCCCAGCCAGG
  GGTGCCCGCTTCCCACCCCTCTCCCCGAGCGGCGTCCCCGTCGGGGTCGAACCTCCCCCG
  CGACAGGCGCCCAGCCGCCTGTTCCCCCAGGCCTTGCCTGCGGACCGCCCTGGGAACGGC
  GTCTGGGAGCCCCGAGGGGCTGGCGGGACTCGGGGCCCGGGCCATGCGGCAGCCGCCCCC
  GCCCCTCCGCGACCATCCCCGGCCCAAGCGGGAGCCGCAGCGGCGTTTCTGCTCCTCGCT
  GCGCCGGCGACCCCGGACCCGGCACCCGTGACCCCTCGCGCTGCCTGCGGCCTCGCGCCG
  AGGCCTGCCCCGGGAGAGACCGTCCATGCCGTCCAAGGCGGAGAATCTGCGGCCCTCCGA
  GCCGGCCCCGCAGCCGCCGGAAGGGAGGACGCTCCAAGGACAGCTTCCCGGCGCTCCGCC
  GGCCCAGCGCGCGGGGTCCCCACCCGATGCTCCGGGCTCCGAGAGCCCCGCGCTTGCCTG
  CAGCACTCCGGCCACGCCCAGCGGCGAGGACCCGCCAGCCCGAGCAGCACCCATCGCCCC
  GCGGCCCCCCGCCAGGCCTCGACTGGAGCGAGCCCTGTCCCTGGACGACAAGGGCTGGAG
  GAGGAGGCGTTTTCGAGGCAGCCAGGAGGACCTGGAAGCCCGGAATGGGACCAGTCCCTC
  CAGGGGCTCAGTGCAGAGCGAGGGCCCCGGGGCCCCCGCCCACAGCTGCTCCCCGCCCTG
  CCTGAGCACCTCCTTGCAGGAAATCCCCAAGTCCCGCGGGGTCCTGAGCAGTGAGAGAGG
  GAGCCCGTCCTCGGGGGGTAACCCTCTCTCTGGGGTGGCCAGCAGCTCCCCGAACCTCCC
  GCACAGAGACGCCGCCGTGGCGGGGAGCTCGCCCAGGCTGCCCAGCCTGCTGCCCCCGCG
  CCCACCGCCTGCCCTGAGCCTGGACATCGCCTCCGACTCCCTGAGGACAGCAAACAAGGT
  CGACTCGGATCTTGCAGACTACAAGCTCCGCGCGCAGCCGCTCCTGGTGCGGGCCCACAG
  CAGCCTGGGCCCCGGCCGGCCGCGGAGCCCCCTGGCCTGCGACGACTGTTCCCTTCGCTC
  GGCCAAATCCTCCTTCAGCCTCCTGGCGCCCATCCGCAGCAAGGACGTCCGCAGCAGGAG
  TTACCTGGAGGGCAGCCTCCTGGCCAGCGGGGCCCTGTTGGGGGCGGATGAGCTGGCCCG
  CTACTTCCCAGACCGGAACGTGGCACTCTTCGTGGCCACCTGGAACATGCAGGGCCAGAA
  GGAGCTCCCGCCCAGCCTGGACGAGTTCCTGCTCCCAGCCGAGGCCGACTATGCCCAGGA
  CCTGTATGTCATCGGGGTCCAGGAGGGCTGTTCTGACAGGCGGGAGTGGGAGACTCGTCT
  GCAGGAGACGCTGGGCCCGCACTATGTGCTGCTGTCCTCGGCGGCCCACGGCGTGCTCTA
  CATGTCGCTCTTCATCCGCAGGGACCTCATCTGGTTCTGCTCAGAGGTGGAGTGCTCCAC
  GGTGACCACACGCATCGTGTCTCAGATCAAGACCAAGGGGGCCTTGGGCATCAGCTTCAC
  CTTTTTTGGCACTTCCTTCCTCTTCATCACGTCCCACTTCACCTCAGGTGACGGGAAGGT
  GGCGGAGCGGCTGCTGGACTACACCAGGACTGTACAAGCCCTGGTCCTGCCCAGAAATGT
  GCCCGACACCAACCCCTATCGCTCCAGCGCAGCGGACGTCACCACCCGCTTCGATGAGGT
  GTTCTGGTTTGGAGACTTCAACTTCCGCCTGAGTGGCGGGCGCACAGTCGTGGACGCCCT
  CCTGTGCCAGGGCCTGGTGGTGGACGTGCCGGCGCTGCTGCAGCACGACCAGCTCATCCG
  GGAGATGCGGAAAGGGTCCATCTTCAAGGGCTTCCAGGAGCCGGACATCCACTTCCTCCC
  ATCATACAAGTTTGACATCGGGAAGGACACGTACGACAGCACCTCCAAGCAGAGGACGCC
  CTCATACACGGACCGCGTCTTGTACAGAAGCCGCCACAAGGGTGACATCTGTCCTGTGAG
  CTACTCTTCCTGCCCCGGGATCAAGACGTCCGACCACCGCCCTGTGTATGGCCTCTTCCG
  GGTGAAAGTGAGGCCGGGGCGAGACAACATTCCGTTGGCAGCTGGCAAATTTGATAGAGA
  ACTGTACTTACTAGGAATTAAAAGACGGATTTCGAAGGAGATTCAGAGGCAGCAAGCACT
  ACAGAGTCAGAACTCCAGCACCATCTGCTCCGTTTCTTGAAGTTTGCTGAACGAGGACTC
  ACAGCTGCAACGTGGGGTGATTGTATTGATCAAAACCCACTGGGAAGGACAAAGAGTTTG
  CCGCCTTTCGGGGATCCGAGGGACTGTGGCGACCGTGCCTCTGTGCCAGCGTCCCAGGAA
  GGAAGCCAACCCTGAGCGAGCCTGTCCTCTGTGGCAGGTCCACACGGTGTGGGTGGGCAG
  GGCTTGGACCCCCGTCTCCATGGCAGGTCCATACAGCATGGGTGGCAGGGTTTGGACCCG
  CCCAGCAGCACCACGGACCCCAGCCACTCTCGGGGGCAGACGTCAGAATCCGTTCCTGAG
  CAGCTCCTGTGCCCTGGGGGCAGTCACAGAGCCCCCCAACACCCCCGTGCTCTGCACCAG
  CCTCTCCCTCCACACCCGAAGCAGGCGTCCATCTGTGTCCTCCTGGCAGCCCCTCAAACA
  CACACCACCCCATGTCACACGGGTATCCAGAGCAAGGCTGGATCTGGGCTAGGTGACCCC
  TGGGGCCTCAGACCTCTGAGTGGGCCCCAGAGCCCAGCTGAGGAAGCCACACAGCCTCCA
  GGACGTGGCACGGTTCCCCTCCACTTGGCTGCAAGTCGCCTGCCATGGGGCGAGGAGGCC
  TGCACCCCAGTATAAGGGCCTCCCCAGCTGAGTAAGAAGCTGCTTCCCCTCCTCTCATAG
  GCCAAGCCTATTGTGTGAAACCATCTCATGGTCTTGGTGACGTAGACCATTTTTGAAACC
  GTCTCATGGTCTTGGTGACGTAGACCGTTTGCTTCTTTAACTCCAGCCGCGGAATGACAT
  TAGTGGAACCGGGCTAGGGAACTGCTGGAAGTTCAGGATGCCACCACCTTGAACACCTAG
  GCCAGGGATCCCCACCATGTCCCGGGTTTCTTTCTTCGAGAGTATAGAACCGTTCATTCT
  TGCTTTGTGTCCCATTCCATCTCTTGAAAAAATGTAGTCTTTGAATGTGTGAAAATCTAG
  GGACATTCAATCTAGTCTTTTAAATTATTTTTCCATATTTATATTTTTAAATAAATGCAT
  ATATTGAGTCTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAA
• Restriction Sites: ECoRI-NOT
• ACCN: NM_019892
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019892.3, NP_063945.2
• RefSeq Size: 3440 bp
• RefSeq ORF: 1935 bp
• Locus ID: 56623
• UniProt ID: Q9NRR6
• Cytogenetics: 9q34.3
• Protein Families: Druggable Genome
• Protein Pathways: Inositol phosphate metabolism, Metabolic pathways, Phosphatidylinositol signaling system
• Gene Summary: The protein encoded by this gene is an inositol 1,4,5-trisphosphate (InsP3) 5-phosphatase. InsP3 5-phosphatases hydrolyze Ins(1,4,5)P3, which mobilizes intracellular calcium and acts as a second messenger mediating cell responses to various stimulation. Studies of the mouse counterpart suggest that this protein may hydrolyze phosphatidylinositol 3,4,5-trisphosphate and phosphatidylinositol 3,5-bisphosphate on the cytoplasmic Golgi membrane and thereby regulate Golgi-vesicular trafficking. Mutations in this gene cause Joubert syndrome; a clinically and genetically heterogenous group of disorders characterized by midbrain-hindbrain malformation and various associated ciliopathies that include retinal dystrophy, nephronophthisis, liver fibrosis and polydactyly. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2016]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).