CREB1 (NM_134442) Human Untagged Clone

SKU SC323817

CREB1 (untagged)-Human cAMP responsive element binding protein 1 (CREB1), transcript variant B

Product Data

• Type: Human Untagged Clone
• Target Symbol: CREB1
• Synonyms: CREB; CREB-1
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_134442.2 GCGGTGTGTTACGTGGGGGAGAGAATAAAACTCCAGCGAGATCCGGGCCGTGAACGAAAG; CAGTGACGGAGGAGCTTGTACCACCGGTAACTAAATGACCATGGAATCTGGAGCCGAGAA; CCAGCAGAGTGGAGATGCAGCTGTAACAGAAGCTGAAAACCAACAAATGACAGTTCAAGC; CCAGCCACAGATTGCCACATTAGCCCAGGTATCTATGCCAGCAGCTCATGCAACATCATC; TGCTCCCACCGTAACTCTAGTACAGCTGCCCAATGGGCAGACAGTTCAAGTCCATGGAGT; CATTCAGGCGGCCCAGCCATCAGTTATTCAGTCTCCACAAGTCCAAACAGTTCAGTCTTC; CTGTAAGGACTTAAAAAGACTTTTCTCCGGAACACAGATTTCAACTATTGCAGAAAGTGA; AGATTCACAGGAGTCAGTGGATAGTGTAACTGATTCCCAAAAGCGAAGGGAAATTCTTTC; AAGGAGGCCTTCCTACAGGAAAATTTTGAATGACTTATCTTCTGATGCACCAGGAGTGCC; AAGGATTGAAGAAGAGAAGTCTGAAGAGGAGACTTCAGCACCTGCCATCACCACTGTAAC; GGTGCCAACTCCAATTTACCAAACTAGCAGTGGACAGTATATTGCCATTACCCAGGGAGG; AGCAATACAGCTGGCTAACAATGGTACCGATGGGGTACAGGGCCTGCAAACATTAACCAT; GACCAATGCAGCAGCCACTCAGCCGGGTACTACCATTCTACAGTATGCACAGACCACTGA; TGGACAGCAGATCTTAGTGCCCAGCAACCAAGTTGTTGTTCAAGCTGCCTCTGGAGACGT; ACAAACATACCAGATTCGCACAGCACCCACTAGCACTATTGCCCCTGGAGTTGTTATGGC; ATCCTCCCCAGCACTTCCTACACAGCCTGCTGAAGAAGCAGCACGAAAGAGAGAGGTCCG; TCTAATGAAGAACAGGGAAGCAGCTCGAGAGTGTCGTAGAAAGAAGAAAGAATATGTGAA; ATGTTTAGAAAACAGAGTGGCAGTGCTTGAAAATCAAAACAAGACATTGATTGAGGAGCT; AAAAGCACTTAAGGACCTTTACTGCCACAAATCAGATTAATTTGGGATTTAAATTTTCAC; CTGTTAAGGTGGAAAATGGACTGGCTTGGCCACAACCTGAAAGACAAAATAAACATTTTA; TTTTCTAAACATTTCTTTTTTTCTATGCGCAAAACTGCCTGAAAGCAACTACAGAATTTC; ATTCATTTGTGCTTTTGCATTAAACTGTGAATGTTCCAACACCTGCCTCCACTTCTCCCC; TCAAGAAATTTTCAACGCCAGGAATCATGAAGAGACTTCTGCTTTTCAACCCCCACCCTC; CTCAAGAAGTAATAATTTGTTTACTTGTAAATTGATGGGAGAAATGAGGAAAAGAAAATC; TTTTTAAAAATGATTTCAAGGTTTGTGCTGAGCTCCTTGATTGCCTTAGGGACAGAATTA; CCCCAGCCTCTTGAGCTGAAGTAATGTGTGGGCCGCATGCATAAAGTAAGTAAGGTGCAA; TGAAGAAGTGTTGATTGCCAAATTGACATGTTGTCACATTCTCATTGTGAATTATGTAAA; GTTGTTAAGAGACATACCCTCTAAAAAAGAACTTTAGCATGGTATTGAAGGAATTAGAAA; TGAATTTGGAGTGCTTTTTATGTATGTTGTCTTCTTCAATACTGAAAATTTGTCCTTGGT; TCTTAAAAGCATTCTGTACTAATACAGCTCTTCCATAGGGCAGTTGTTGCTTCTTAATTC; AGTTCTGTATGTGTTCAACATTTTTGAATACATTAAAAGAAGTAACCAACTGAACGACAA; AGCATGGTATTTGAATTTTAAATTAAAGCAAAGTAAATAAAAGTACAAAGCATATTTTAG; TTAGTACTAAATTCTTAGTAAAATGCTGATCAGTAAACCAATCCCTTGAGTTATATAACA; AGATTTTTAAATAAATGTTATTGTCCTCACCTTCAAAAATATTTATATTGTCACTCATTT; ACGTAAAAAGATATTTCTAATTTACTGTTGCCCATTGCACTTACATACCACCACCAAGAA; AGCCTTCAAGATGTCAAATAAAGCAAAGTGATATATATTTGTTTATGAAATGTTACATGT; AGAAAAATACTGATTTTAAATATTTTCCATATTAACAATTTAACAGAGAATCTCTAGTGA; ATTTTTTAAATGAAAGAAGTTGTAAGGATATAAAAAGTACAGTGTTAGATGTGCACAAGG; AAAGTTATTTTCAGACATATTTGAATGACTGCTGTACTGCAATATTTGGATTGTCATTCT; TACAAAACATTTTTTTGTTCTCTTGTAAAAAGAGTAGTTATTAGTTCTGCTTTAGCTTTC; CAATATGCTGTATAGCCTTTGTCATTTTATAATTTTAATTCCTGATTAAAACAGTCTGTA; TTTGTGTATATCATACATTGTTTTCAATACCACTTTTAATTGTTACTCATTTTATTCACT; AAGCTCGATAAATCTAACAGTTACTCTTAAAAAAAAAAAAAAGACTAAGGTGGATTTTAA; AAATTGGAAACTGACATAATGTTAGGTTATAATTTCTCATTTGGAGCCGGGCGCAGTGGC; TCACGCCTGTAATCCCAGCACTTTGGGAGGCCAAGGTGGGTGGATCACCTGTGGTCAAGA; GTTCAAGACCAGCCTGGCCATCATGGTGAAACCCCATCTCTACTAAAAATACAAAAATTA; GCCAGGCGTGGTGGCTGGCGCCTGTAATCTCAGCTCCTCAGGAGCTTGAGGCAGCAGAAT; TGCTTGAACCCAGGAGGCAGAGGGTTGCAGTGAGCCGAGATAGCACCATTGCACTCCAGC; CTGGGCGACTCCATCTCAAAAAATAAAAAAAAAAAAAAA
• Restriction Sites: ECoRI-NOT
• ACCN: NM_134442
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_134442.2, NP_604391.1
• RefSeq Size: 3006 bp
• RefSeq ORF: 1026 bp
• Locus ID: 1385
• UniProt ID: P16220
• Cytogenetics: 2q33.3
• Domains: BRLZ, pKID
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Antigen processing and presentation, Huntington's disease, Melanogenesis, Prostate cancer
• Summary: This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in several transcript variants encoding different isoforms. [provided by RefSeq, Mar 2016]; Transcript Variant: This variant (2, also known as B) represents the longest transcript and encodes the longest isoform (B). Sequence Note: The RefSeq transcript and protein were derived from transcript and genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.