PRMT7 (NM_019023) Human Untagged Clone

CAT#: SC323779

PRMT7 (untagged)-Human protein arginine methyltransferase 7 (PRMT7), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PRMT7
• Synonyms: SBIDDS
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_019023.1 GGATTTCTGACAGTCAGACTTGTCCACAAGAACTCAACTGGCAAGGCTGCTTTTCTGTGC
  TAAAACTGGGGAGCTAGTGGGCACCATGAAGATCTTCTGCAGTCGGGCCAATCCGACCAC
  GGGGTCTGTGGAGTGGCTGGAGGAGGATGAACACTATGATTACCACCAGGAGATTGCAAG
  GTCATCTTATGCAGATATGCTACATGACAAAGACAGAAATGTAAAATACTACCAAGGTAT
  CCGGGCTGCCGTGAGCAGGGTGAAGGACAGAGGACAGAAGGCCTTGGTTCTCGACATTGG
  CACTGGCACGGGACTCTTGTCAATGATGGCGGTCACAGCAGGTGCCGACTTCTGCTATGC
  CATCGAGGTTTTCAAGCCTATGGCTGATGCTGCTGTGAAGATTGTGGAGAAAAATGGCTT
  TAGTGATAAGATTAAGGTTATCAACAAGCATTCCACCGAGGTGACTGTAGGTCCAGAGGG
  TGACATGCCATGCCGTGCCAACATCCTGGTCACAGAGTTGTTTGACACAGAGCTGATCGG
  GGAGGGGGCGCTGCCCTCCTATGAGCACGCACACAGGCATCTCGTGGAGGAAAATTGTGA
  GGCCGTGCCCCACAGAGCCACCGTCTATGCACAGCTGGTGGAGTCCGGGAGGATGTGGTC
  GTGGAACAAGCTATTTCCCATCCACGTGCAGACCAGCCTCGGAGAGCAGGTCATCGTCCC
  TCCCGTTGACGTGGAGAGCTGCCCTGGCGCACCCTCTGTCTGTGACATTCAGCTGAACCA
  GGTGTCACCAGCCGACTTTACAGTCCTCAGCGATGTGCTGCCCATGTTCAGCATAGACTT
  CAGCAAGCAAGTCAGTAGCTCAGCAGCCTGCCATAGCAGGCGGTTTGAACCTCTGACATC
  TGGCCGAGCTCAGGTGGTTCTCTCGTGGTGGGACATTGAAATGGACCCTGAGGGGAAGAT
  CAAGTGCACCATGGCCCCCTTCTGGGCACACTCAGACCCAGAGGAGATGCAGTGGCGGGA
  CCACTGGATGCAGTGTGTGTACTTCCTGCCACAAGAGGAGCCTGTGGTGCAGGGCTCAGC
  GCTCTATCTGGTAGCCCACCACGATGACTACTGCGTATGGTACAGCCTGCAGAGGACCAG
  CCCTGAAAAGAATGAGAGAGTCCGCCAGATGCGCCCCGTGTGTGACTGCCAGGCTCACCT
  GCTCTGGAACCGGCCTCGGTTTGGAGAGATCAATGACCAGGACAGAACTGATCGATACGT
  CCAGGCTCTGAGGACCGTGCTGAAGCCAGACAGCGTGTGCCTGTGTGTCAGCGATGGCAG
  CCTGCTCTCCGTGCTGGCCCATCACCTGGGGGTGGAGCAGGTGTTTACAGTCGAGAGTTC
  AGCAGCTTCTCACAAACTGTTGAGAAAAATCTTCAAGGCTAACCACTTGGAAGATAAAAT
  TAACATCATAGAGAAACGGCCGGAATTATTAACAAATGAGGACCTACAGGGCAGAAAGGT
  CTCTCTCCTCCTGGGCGAGCCGTTCTTCACTACCAGCCTGCTGCCGTGGCACAACCTCTA
  CTTCTGGTACGTGCGGACCGCTGTGGACCAGCACCTGGGGCCAGGTGCCATGGTGATGCC
  CCAGGCAGCCTCGCTGCACGCTGTGGTTGTGGAGTTCAGGGACCTGTGGCGGATCCGGAG
  CCCCTGTGGTGACTGCGAAGGCTTCGACGTGCACATCATGGACGACATGATTAAGCGTGC
  CCTGGACTTCAGGGAGAGCAGGGAAGCTGAGCCCCACCCGCTGTGGGAGTACCCATGCCG
  CAGCCTCTCCGAGCCCTGGCAGATCCTGACCTTTGACTTCCAGCAGCCGGTGCCCCTGCA
  GCCCCTGTGTGCCGAGGGCACTGTGGAGCTCAGAAGGCCCGGGCAGAGCCACGCAGCGGT
  GCTATGGATGGAGTACCACCTGACCCCGGAGTGCACGCTCAGCACTGGCCTCCTGGAGCC
  TGCAGACCCCGAGGGGGGCTGCTGCTGGAACCCCCACTGCAAGCAGGCCGTCTACTTCTT
  CAGCCCTGCCCCAGATCCCAGAGCACTGCTGGGTGGCCCACGGACTGTCAGCTATGCAGT
  GGAGTTTCACCCCGACACAGGCGACATCATCATGGAGTTCAGGCATGCAGATACCCCAGA
  CTGACCACTCTTGAGCAATAAAAGGGCCTGAGGGCTGGGAAAAAAAAAAAAAAAAAAACC
  AAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: ECoRI-NOT
• ACCN: NM_019023
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019023.1, NP_061896.1
• RefSeq Size: 2390 bp
• RefSeq ORF: 2079 bp
• Locus ID: 54496
• UniProt ID: Q9NVM4
• Cytogenetics: 16q22.1
• Protein Families: Druggable Genome
• Gene Summary: This gene encodes a member of the protein arginine N-methyltransferase family of proteins. The encoded enzyme transfers single methyl groups to arginine residues to generate monomethylarginines on histone proteins as well as other protein substrates. This enzyme plays a role in a wide range of biological processes, including neuronal differentiation, male germ line imprinting, small nuclear ribonucleoprotein biogenesis, and regulation of the Wnt signaling pathway. Mutations in this gene underlie multiple related syndromes in human patients characterized by intellectual disability, short stature and other features. The encoded protein may promote breast cancer cell invasion and metastasis in human patients. [provided by RefSeq, May 2017]
  Transcript Variant: This variant (1) includes a 3' terminal exon that extends past a splice site that is used in variant 4, which results in a novel 3' coding region and 3' UTR compared to variant 4. The encoded isoform (1) is shorter and has a distinct C-terminus compared to isoform 4. Both variants 1 and 3 encode the same isoform (1).