RYK (NM_002958) Human Untagged Clone

SKU SC323660

RYK (untagged)-Kinase deficient mutant (K234M) of Human RYK receptor-like tyrosine kinase (RYK), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: RYK
• Synonyms: D3S3195; JTK5; JTK5A; RYK1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_002958, the custom clone sequence may differ by one or more nucleotides; ; ; ATGCGTGGGGCGGCGCGGCTGGGGCGGCCGGGCCGGAGTTGCCTCCCGGGGGCCCGCGGCCTGAGGGCCC; CGCCGCCGCCGCCGCTGCTGCTTCTGCTTGCGCTGTTGCCGCTGCTGCCCGCGCCTGGCGCTGCCGCCGC; CCCCGCCCCGCGGCCCCCGGAGCTGCAGTCGGCTTCCGCGGGGCCCAGCGTGAGTCTCTACCTGAGCGAG; GACGAGGTGCGCCGGCTGATCGGTCTTGATGCAGAACTTTATTATGTGAGAAATGACCTTATTAGTCACT; ACGCTCTATCCTTTAGTCTGTTAGTACCCAGTGAGACAAATTTCCTGCACTTCACCTGGCATGCGAAGTC; CAAGGTTGAATATAAGCTGGGATTCCAAGTGGACAATGTTTTGGCAATGGATATGCCCCAGGTCAACATT; TCTGTTCAGGGGGAAGTTCCACGCACTTTATCAGTGTTTCGGGTAGAGCTTTCCTGTACTGGCAAAGTAG; ATTCTGAAGTTATGATACTAATGCAGCTCAACTTGACAGTAAATTCTTCAAAAAATTTTACCGTCTTAAA; TTTTAAACGAAGGAAAATGTGCTACAAAAAACTTGAAGAAGTAAAAACTTCAGCCTTGGACAAAAACACT; AGCAGAACTATTTATGATCCTGTACATGCAGCTCCAACCACTTCTACGCGTGTGTTTTATATTAGTGTAG; GGGTTTGTTGTGCAGTAATATTTCTCGTAGCAATAATATTAGCTGTTTTGCACCTTCATAGTATGAAAAG; GATTGAACTGGATGACAGCATTAGTGCCAGCAGTAGTTCCCAAGGGCTGTCTCAGCCATCCACCCAGACG; ACTCAGTATCTGAGAGCAGACACGCCCAACAATGCAACTCCTATCACCAGTTATCCTACCTTGCGGATAG; AGAAGAACGACTTGAGAAGTGTCACTCTTTTGGAGGCCAAAGGCAAGGTGAAGGATATAGCAATATCCAG; AGAGAGGATAACTCTAAAAGATGTACTCCAAGAAGGTACTTTTGGGCGTATTTTCCATGGGATTTTAATA; GATGAAAAAGATCCAAATAAAGAAAAACAAGCATTTGTCAAAACAGTTAAAGATCAAGCTTCTGAAATTC; AGGTGACAATGATGCTCACTGAAAGTTGTAAGCTGCGAGGTCTTCATCACAGAAATCTTCTTCCTATTAC; TCATGTGTGTATAGAAGAAGGAGAAAAGCCCATGGTGATATTGCCTTACATGAATTGGGGGAATCTTAAA; TTGTTTTTACGACAGTGCAAGTTAGTAGAGGCCAATAATCCACAGGCAATTTCTCAGCAAGACCTGGTAC; ACATGGCTATTCAGATTGCCTGTGGAATGAGCTACCTGGCCAGAAGGGAAGTCATCCACAAAGACCTGGC; TGCCAGGAACTGTGTCATTGATGACACACTTCAAGTTAAGATCACAGACAATGCCCTCTCCAGAGACTTG; TTCCCCATGGACTATCACTGTCTGGGGGACAATGAAAACAGGCCAGTTCGTTGGATGGCTCTTGAAAGTC; TGGTTAATAACGAGTTCTCTAGCGCTAGTGATGTGTGGGCCTTTGGAGTGACGCTGTGGGAACTCATGAC; TCTGGGCCAGACTCCCTACGTGGACATTGACCCCTTCGAGATGGCCGCATACCTGAAAGATGGTTACCGA; ATAGCCCAGCCAATCAACTGTCCTGATGAATTATTTGCTGTGATGGCCTGTTGCTGGGCCTTAGATCCAG; AGGAGAGGCCCAAGTTTCAGCAGCTGGTACAGTGCCTAACAGAGTTTCATGCAGCCCTGGGGGCCTACGT; CTGA; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_002958 unedited; CCCGCCGTTTGAGCAATGGGCGGTAGGCGTGTACGGTTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTG; AACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCGCGTCCCGCG; GGCAGCCCCCAACTCCGGACGATGCAGCGAGGAGGCGGCGGCGGCGGCGCGCTCCGGAGGTCTGAAGCCA; CTGCTGGCTGCCACGGGCCGGCGGTGCGATGAGCTGGGCAGCCGCCCCCGCCCCGCGGCCCCCGGAGCTG; CAGTCGGCTTCCGCGGGGCCCAGCGTGAGTCTCTACCTGAGCGAGGACGAGTGCGCCGCTGATCGTCTTG; ATGCAGACTTTATTATGTGAAAAATGACCTTATTAATTCACTAACGCCTCCTATCCCTTTATTCTTGGTT; ATTACCCATTGAACACAAATTTCCCTGCACCTTCCACCTTGGCATGCCCGAAGTCCCAGGGTGGGAATAA; TAGGCCTTGGAAATTCCAGGGTGGGACAAGGTTTTTGCCAAGGAGATATGCCCCCAGGTCACACATTTTT; GGTTTCGGGGGGAAATTTCCCCCCCCTTTTTTAGTGTGTTTCGGGGAAACGTTTTCCGGGGCGGGGCAAG; TTATATTTGTAAGTTTTGAAATCAAAGGGCCCCCTAACTTTGGCGGGAAATTTTTCTCAAAAATTTTTTC; CCCTTTTAATTTTTTTAAACAAAGGGGAAAGGGGGCTAAAAAAAATTTTGAAAAATATTAAAACTCTTTC; CCCTGTGGGAAAAAAACCCCTCACAAAACACCAATAAAAAACCGGACAACAGGCGCCCCCCCCTTTTTCT; AGCCGCGGGGTAAAAAAAAGGCGGGGGGGTCTGGGTCCACAAATTTTTTCTCACACCAATTATTATCTGT; TTTGCCCCCCACATATAGAGAGAATATACTGTCGAGACAGG;
  Kinase Domain Sequence

  >SC323660 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation; GMTCCAGATGTACTTTTGGGCGTATTTTCCATGGGATTTTAATAGATGAAAAAGATCCAAATAAAGAAAA; ACAAGCATTTGTCATGACAGTTAAAGATCAAGCTTCTGAAATTCAGGTGACAATGATGCTCACTGAAAGT; TGTAAGCTGCGAGGTCTTCATCACAGAAATCTTCTTCCTATTACTCATGTGTGTATAGAAGAAGGAGAAA; AGCCCATGGTGATATTGCCTTACATGAATTGGGGGAATCTTAAAT;
• Restriction Sites: Please inquire
• ACCN: NM_002958
• Insert Size: 3100 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_002958.2, NP_002949.2
• RefSeq Size: 2951 bp
• RefSeq ORF: 1824 bp
• Locus ID: 6259
• UniProt ID: P34925
• Cytogenetics: 3q22.2
• Domains: pkinase, S_TKc, TyrKc, WIF
• Protein Families: Druggable Genome, Protein Kinase, Transmembrane
• Summary: The protein encoded by this gene is an atypical member of the family of growth factor receptor protein tyrosine kinases, differing from other members at a number of conserved residues in the activation and nucleotide binding domains. This gene product belongs to a subfamily whose members do not appear to be regulated by phosphorylation in the activation segment. It has been suggested that mediation of biological activity by recruitment of a signaling-competent auxiliary protein may occur through an as yet uncharacterized mechanism. The encoded protein has a leucine-rich extracellular domain with a WIF-type Wnt binding region, a single transmembrane domain, and an intracellular tyrosine kinase domain. This protein is involved in stimulating Wnt signaling pathways such as the regulation of axon pathfinding. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Feb 2012]; Transcript Variant: This variant (2) uses an alternate in-frame splice site in the coding region, compared to variant 1, resulting in a shorter protein (isoform 2).