TIE1 (NM_005424) Human Untagged Clone

SKU SC323581

TIE1 (untagged)-Kinase deficient mutant (K870M) of Human tyrosine kinase with immunoglobulin-like and EGF-like domains 1 (TIE1)

Product Data

• Type: Human Untagged Clone
• Target Symbol: TIE1
• Synonyms: JTK14; LMPHM11; TIE
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC323581 sequence for NM_005424 edited (data generated by NextGen Sequencing); ATGGTCTGGCGGGTGCCCCCTTTCTTGCTCCCCATCCTCTTCTTGGCTTCTCATGTGGGC; GCGGCGGTGGACCTGACGCTGCTGGCCAACCTGCGGCTCACGGACCCCCAGCGCTTCTTC; CTGACTTGCGTGTCTGGGGAGGCCGGGGCGGGGAGGGGCTCGGACGCCTGGGGCCCGCCC; CTGCTGCTGGAGAAGGACGACCGTATCGTGCGCACCCCGCCCGGGCCACCCCTGCGCCTG; GCGCGCAACGGTTCGCACCAGGTCACGCTTCGCGGCTTCTCCAAGCCCTCGGACCTCGTG; GGCGTCTTCTCCTGCGTGGGCGGTGCTGGGGCGCGGCGCACGCGCGTCATCTACGTGCAC; AACAGCCCTGGAGCCCACCTGCTTCCAGACAAGGTCACACACACTGTGAACAAAGGTGAC; ACCGCTGTACTTTCTGCACGTGTGCACAAGGAGAAGCAGACAGACGTGATCTGGAAGAGC; AACGGATCCTACTTCTACACCCTGGACTGGCATGAAGCCCAGGATGGGCGGTTCCTGCTG; CAGCTCCCAAATGTGCAGCCACCATCGAGCGGCATCTACAGTGCCACTTACCTGGAAGCC; AGCCCCCTGGGCAGCGCCTTCTTTCGGCTCATCGTGCGGGGTTGTGGGGCTGGGCGCTGG; GGGCCAGGCTGTACCAAGGAGTGCCCAGGTTGCCTACATGGAGGTGTCTGCCACGACCAT; GACGGCGAATGTGTATGCCCCCCTGGCTTCACTGGCACCCGCTGTGAACAGGCCTGCAGA; GAGGGCCGTTTTGGGCAGAGCTGCCAGGAGCAGTGCCCAGGCATATCAGGCTGCCGGGGC; CTCACCTTCTGCCTCCCAGACCCCTATGGCTGCTCTTGTGGATCTGGCTGGAGAGGAAGC; CAGTGCCAAGAAGCTTGTGCCCCTGGTCATTTTGGGGCTGATTGCCGACTCCAGTGCCAG; TGTCAGAATGGTGGCACTTGTGACCGGTTCAGTGGTTGTGTCTGCCCCTCTGGGTGGCAT; GGAGTGCACTGTGAGAAGTCAGACCGGATCCCCCAGATCCTCAACATGGCCTCAGAACTG; GAGTTCAACTTAGAGACGATGCCCCGGATCAACTGTGCAGCTGCAGGGAACCCCTTCCCC; GTGCGGGGCAGCATAGAGCTACGCAAGCCAGACGGCACTGTGCTCCTGTCCACCAAGGCC; ATTGTGGAGCCAGAGAAGACCACAGCTGAGTTCGAGGTGCCCCGCTTGGTTCTTGCGGAC; AGTGGGTTCTGGGAGTGCCGTGTGTCCACATCTGGCGGCCAAGACAGCCGGCGCTTCAAG; GTCAATGTGAAAGTGCCCCCCGTGCCCCTGGCTGCACCTCGGCTCCTGACCAAGCAGAGC; CGCCAGCTTGTGGTCTCCCCGCTGGTCTCGTTCTCTGGGGATGGACCCATCTCCACTGTC; CGCCTGCACTACCGGCCCCAGGACAGTACCATGGACTGGTCGACCATTGTGGTGGACCCC; AGTGAGAACGTGACGTTAATGAACCTGAGGCCAAAGACAGGATACAGTGTTCGTGTGCAG; CTGAGCCGGCCAGGGGAAGGAGGAGAGGGGGCCTGGGGGCCTCCCACCCTCATGACCACA; GACTGTCCTGAGCCTTTGTTGCAGCCGTGGTTGGAGGGCTGGCATGTGGAAGGCACTGAC; CGGCTGCGAGTGAGCTGGTCCTTGCCCTTGGTGCCCGGGCCACTGGTGGGCGACGGTTTC; CTGCTGCGCCTGTGGGACGGGACACGGGGGCAGGAGCGGCGGGAGAACGTCTCATCCCCC; CAGGCCCGCACTGCCCTCCTGACGGGACTCACGCCTGGCACCCACTACCAGCTGGATGTG; CAGCTCTACCACTGCACCCTCCTGGGCCCGGCCTCGCCCCCTGCACACGTGCTTCTGCCC; CCCAGTGGGCCTCCAGCCCCCCGACACCTCCACGCCCAGGCCCTCTCAGACTCCGAGATC; CAGCTGACATGGAAGCACCCGGAGGCTCTGCCTGGGCCAATATCCAAGTACGTTGTGGAG; GTGCAGGTGGCTGGGGGTGCAGGAGACCCACTGTGGATAGACGTGGACAGGCCTGAGGAG; ACAAGCACCATCATCCGTGGCCTCAACGCCAGCACGCGCTACCTCTTCCGCATGCGGGCC; AGCATTCAGGGGCTCGGGGACTGGAGCAACACAGTAGAAGAGTCCACCCTGGGCAACGGG; CTGCAGGCTGAGGGCCCAGTCCAAGAGAGCCGGGCAGCTGAAGAGGGCCTGGATCAGCAG; CTGATCCTGGCGGTGGTGGGCTCCGTGTCTGCCACCTGCCTCACCATCCTGGCCGCCCTT; TTAACCCTGGTGTGCATCCGCAGAAGCTGCCTGCATCGGAGACGCACCTTCACCTACCAG; TCAGGCTCGGGCGAGGAGACCATCCTGCAGTTCAGCTCAGGGACCTTGACACTTACCCGG; CGGCCAAAACTGCAGCCCGAGCCCCTGAGCTACCCAGTGCTAGAGTGGGAGGACATCACC; TTTGAGGACCTCATCGGGGAGGGGAACTTCGGCCAGGTCATCCGGGCCATGATCAAGAAG; GACGGGCTGAAGATGAACGCAGCCATCATGATGCTGAAAGAGTATGCCTCTGAAAATGAC; CATCGTGACTTTGCGGGAGAACTGGAAGTTCTGTGCAAATTGGGGCATCACCCCAACATC; ATCAACCTCCTGGGGGCCTGTAAGAACCGAGGTTACTTGTATATCGCTATTGAATATGCC; CCCTACGGGAACCTGCTAGATTTTCTGCGGAAAAGCCGGGTCCTAGAGACTGACCCAGCT; TTTGCTCGAGAGCATGGGACAGCCTCTACCCTTAGCTCCCGGCAGCTGCTGCGTTTCGCC; AGTGATGCGGCCAATGGCATGCAGTACCTGAGTGAGAAGCAGTTCATCCACAGGGACCTG; GCTGCCCGGAATGTGCTGGTCGGAGAGAACCTAGCCTCCAAGATTGCAGACTTCGGCCTT; TCTCGGGGAGAGGAGGTTTATGTGAAGAAGACGATGGGGCGTCTCCCTGTGCGCTGGATG; GCCATTGAGTCCCTGAACTACAGTGTCTATACCACCAAGAGTGATGTCTGGTCCTTTGGA; GTCCTTCTTTGGGAGATAGTGAGCCTTGGAGGTACACCCTACTGTGGCATGACCTGTGCC; GAGCTCTATGAAAAGCTGCCCCAGGGCTACCGCATGGAGCAGCCTCGAAACTGTGACGAT; GAAGTGTACGAGCTGATGCGTCAGTGCTGGCGGGACCGTCCCTATGAGCGACCCCCCTTT; GCCCAGATTGCGCTACAGCTAGGCCGCATGCTGGAAGCCAGGAAGGCCTATGTGAACATG; TCGCTGTTTGAGAACTTCACTTACGCGGGCATTGATGCCACAGCTGAGGAGGCCTGA; ; Clone variation with respect to NM_005424.2; 2609 a=>t;2610 a=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_005424 unedited; ACGCCCGTACAGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGACTATATAAGCAGAGCTCATATAGGCGA; CACTATAGAATACAAGCTACTCGTACTTTTTGCAGCGGCCGCGAATACGGCACGAGGAGCATCTGACCCC; AGGCCCAGCTCGTCCTGGCTGGCCTGGGTCGGCCTCTGGAGTATGGTCTGGCGGGTGCCCCCTTTCTTGC; TCCCCATCCTCTTCTTGGCTTCTCATGTGGGCGCGGCGGTGGACCTGACGCTGCTGGCCAACCTGCGGCT; CACGGACCCCCAGCGCTTCTTCCTGACTTGCGTGTCTGGGGGAGGGCCGGGGGCGGGGGAAGGGCCTCGG; ACGCCTGGGCCCCGCCCCTGGCTGCTGGAGAAGACCAAACCGTATCGTTGCCCCCCCCCCCCGGCCCACC; CCCTGCCCCTGGCCCCCCAACGGGTTCCAACCAGTTCCCCCTCCGGGGCCTTCCCCAACCCCCTCGAACC; CTCTTGGGCCTTTTTTCTCCGGCTTGGCCGGGGCCTGGGGCCCCGCCCAACCCCCTTTATTTTACTGTCC; CAAAACCCCCGGGACCCCCCCGTGTTTTCCAAAAAGGGTCCCACCCCCTGGAAACAAGGTGTCACCCCCC; TTTTCTTTTTGCCCTTTGTGCCCGGGGAAACCAAAAACTTTTTTCTAGAAAAACACGTTTTCTTTTTTTC; CCCCGAAATGGAGGGACCCCCAAGGGGGGTGCTCTGTGTACCACCCAATTTGTACCCCCCCTAGGGGGGT; CTCAAGTGTCCCTTTCTAGTGATCACCCACCCCGTGTGACAACACACTTTACGCATATAGTGGGTGGTGT; GGGAGTAGCGATCAAGAGACAAAATTAAAAGACAGTTCAATTTA;
  Kinase Domain Sequence

  >SC323581 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation; TKARGMTCATCGGGGAGGGGACTTCGGCCAGGTCRTCCGGGCCATGATCAAGAAGGACGGGCTGAAGATG; AACGCAGCCATCATGATGCTGAAAGAGTATGCCTCTGAAAATGACCATCGTGACTTTGCGGGAGAACTGG; AAGTTCTGTGCAAATTGGGGCATCACCCCAACATCATCAACCTCCTGGGGGCCTGTAAGAACCGAGGTTA; CTTGTATATCGCTATTGAATATGCCCCCTACGGGAACCTGCTAGA;
• Restriction Sites: Please inquire
• ACCN: NM_005424
• Insert Size: 4200 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_005424.2, NP_005415.1
• RefSeq Size: 4000 bp
• RefSeq ORF: 3417 bp
• Locus ID: 7075
• UniProt ID: P35590
• Cytogenetics: 1p34.2
• Domains: EGF, FN3, ig, IG, pkinase, S_TKc, TyrKc
• Protein Families: Druggable Genome, Protein Kinase, Transmembrane
• Summary: This gene encodes a member of the tyrosine protein kinase family. The encoded protein plays a critical role in angiogenesis and blood vessel stability by inhibiting angiopoietin 1 signaling through the endothelial receptor tyrosine kinase Tie2. Ectodomain cleavage of the encoded protein relieves inhibition of Tie2 and is mediated by multiple factors including vascular endothelial growth factor. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011]; Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).