Chk1 (CHEK1) (NM_001274) Human Untagged Clone

SKU SC323414

CHEK1 (untagged)-Kinase deficient mutant (K38M) of Human CHK1 checkpoint homolog (S. pombe) (CHEK1), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: Chk1
• Synonyms: CHK1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC323414 sequence for NM_001274 edited (data generated by NextGen Sequencing); ATGGCAGTGCCCTTTGTGGAAGACTGGGACTTGGTGCAAACCCTGGGAGAAGGTGCCTAT; GGAGAAGTTCAACTTGCTGTGAATAGAGTAACTGAAGAAGCAGTCGCAGTGATGATTGTA; GATATGAAGCGTGCCGTAGACTGTCCAGAAAATATTAAGAAAGAGATCTGTATCAATAAA; ATGCTAAATCATGAAAATGTAGTAAAATTCTATGGTCACAGGAGAGAAGGCAATATCCAA; TATTTATTTCTGGAGTACTGTAGTGGAGGAGAGCTTTTTGACAGAATAGAGCCAGACATA; GGCATGCCTGAACCAGATGCTCAGAGATTCTTCCATCAACTCATGGCAGGGGTGGTTTAT; CTGCATGGTATTGGAATAACTCACAGGGATATTAAACCAGAAAATCTTCTGTTGGATGAA; AGGGATAACCTCAAAATCTCAGACTTTGGCTTGGCAACAGTATTTCGGTATAATAATCGT; GAGCGTTTGTTGAACAAGATGTGTGGTACTTTACCATATGTTGCTCCAGAACTTCTGAAG; AGAAGAGAATTTCATGCAGAACCAGTTGATGTTTGGTCCTGTGGAATAGTACTTACTGCA; ATGCTCGCTGGAGAATTGCCATGGGACCAACCCAGTGACAGCTGTCAGGAGTATTCTGAC; TGGAAAGAAAAAAAAACATACCTCAACCCTTGGAAAAAAATCGATTCTGCTCCTCTAGCT; CTGCTGCATAAAATCTTAGTTGAGAATCCATCAGCAAGAATTACCATTCCAGACATCAAA; AAAGATAGATGGTACAACAAACCCCTCAAGAAAGGGGCAAAAAGGCCCCGAGTCACTTCA; GGTGGTGTGTCAGAGTCTCCCAGTGGATTTTCTAAGCACATTCAATCCAATTTGGACTTC; TCTCCAGTAAACAGTGCTTCTAGTGAAGAAAATGTGAAGTACTCCAGTTCTCAGCCAGAA; CCCCGCACAGGTCTTTCCTTATGGGATACCAGCCCCTCATACATTGATAAATTGGTACAA; GGGATCAGCTTTTCCCAGCCCACATGTCCTGATCATATGCTTTTGAATAGTCAGTTACTT; GGCACCCCAGGATCCTCACAGAACCCCTGGCAGCGGTTGGTCAAAAGAATGACACGATTC; TTTACCAAATTGGATGCAGACAAATCTTATCAATGCCTGAAAGAGACTTGTGAGAAGTTG; GGCTATCAATGGAAGAAAAGTTGTATGAATCAGGTTACTATATCAACAACTGATAGGAGA; AACAATAAACTCATTTTCAAAGTGAATTTGTTAGAAATGGATGATAAAATATTGGTTGAC; TTCCGGCTTTCTAAGGGTGATGGATTGGAGTTCAAGAGACACTTCCTGAAGATTAAAGGG; AAGCTGATTGATATTGTGAGCAGCCAGAAGGTTTGGCTTCCTGCCACATGA; ; Clone variation with respect to NM_001274.5; 113 a=>t;1411 a=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_001274 unedited; ACCGCCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGA; ACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCATGGCAGTGCCC; TTTGTGGAAGACTGGGACTTGGTGCAAACCCTGGGAGAAGGTGCCTATGGAGAAGTTCAACTTGCTGTGA; ATAGAGTAACTGAAGAAGCAGTCGCAGTGATGATTGTAGATATGAAGCGTGCCGTAGACTGTCCAGAAAA; TATTAAGAAAGAGATCTGTATCAATAAAATGCTAAATCATGAAAAATGTAGTAAAAATTCTATGGTCACA; GGGAGGAGAAAGGCAATATCCAATTATTTTATTTTCTGGAGTTACTGTAGTGGAGAGGAAGAGCCTTTTT; GACAGAATTAAAAGCCAGAACTTAAGGCTGTGCCTGGACCCAAATGCTCAGAGAATCCCTTCCTTCACTT; TCTGGGGCAGGGGTGGGGTTTACTCTGCTTGGTATTTGAATATACTCTCCCAGGAAATATTAAACACAGA; AATCTCTCTGGTTGGGAGTGAAAGGGTAACACCTCAAATCTCTCAACATTTTGGCTGGGCCACCGGTTTT; TCGGTAATAAAATCCGTGAAGCTTTTTTTAAACAAAGTGGTGGGGACTTTTCCCATGTTTTGCCCAAAAT; TTGGAGAAAAGAAAAATTTTTGCGGAACCCCTTTTGTGTGTGGCCCTGGGGAAAAATTTTCCGCGCGCCC; CGGGAATGTCCCGGGCCCCCCCCGCAGCCTCTGAGATACTCTCTGGAGAAAAAACTACTCCTCCTCGTGA; AACATCTCTCGCTACTCCGCGCGAACTCTTGAAACTCCCACATATCTCTCATACTAAAAATGTGCCAACC; CCCAGAGGGAAGACCGCGCTCTGGGGTGGATACCACCGAGGTTATCACGCATACCGTAGTAGTACTTCCA; GCAGCTTG;
  Kinase Domain Sequence

  >SC323414 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation; CSCTGMGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTC; AGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCATGGCAGTGCCCTTTGTG; GAAGACTGGGACTTGGTGCAAACCCTGGGAGAAGGTGCCTATGGAGAAGTTCAACTTGCTGTGAATAGAG; TAACTGAAGAAGCAGTCGCAGTGATGATTGTAGATATGAAGCGTG;
• Restriction Sites: Please inquire
• ACCN: NM_001274
• Insert Size: 3400 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001274.2, NP_001265.1
• RefSeq Size: 2042 bp
• RefSeq ORF: 1431 bp
• Locus ID: 1111
• UniProt ID: O14757
• Cytogenetics: 11q24.2
• Domains: pkinase, S_TKc, TyrKc
• Protein Families: Druggable Genome, Protein Kinase, Stem cell - Pluripotency
• Protein Pathways: Cell cycle, p53 signaling pathway
• Summary: The protein encoded by this gene belongs to the Ser/Thr protein kinase family. It is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. This protein acts to integrate signals from ATM and ATR, two cell cycle proteins involved in DNA damage responses, that also associate with chromatin in meiotic prophase I. Phosphorylation of CDC25A protein phosphatase by this protein is required for cells to delay cell cycle progression in response to double-strand DNA breaks. Several alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Oct 2011]