p38 (MAPK14) (NM_139014) Human Untagged Clone

SKU SC323402

MAPK14 (untagged)-Kinase deficient mutant (K53M) of Human mitogen-activated protein kinase 14 (MAPK14), transcript variant 4

Product Data

• Type: Human Untagged Clone
• Target Symbol: p38
• Synonyms: CSBP; CSBP1; CSBP2; CSPB1; EXIP; Mxi2; p38; p38ALPHA; PRKM14; PRKM15; RK; SAPK2A
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_139014, the custom clone sequence may differ by one or more nucleotides; ; ; ATGTCTCAGGAGAGGCCCACGTTCTACCGGCAGGAGCTGAACAAGACAATCTGGGAGGTGCCCGAGCGTT; ACCAGAACCTGTCTCCAGTGGGCTCTGGCGCCTATGGCTCTGTGTGTGCTGCTTTTGACACAAAAACGGG; GTTACGTGTGGCAGTGAAGAAGCTCTCCAGACCATTTCAGTCCATCATTCATGCGAAAAGAACCTACAGA; GAACTGCGGTTACTTAAACATATGAAACATGAAAATGTGATTGGTCTGTTGGACGTTTTTACACCTGCAA; GGTCTCTGGAGGAATTCAATGATGTGTATCTGGTGACCCATCTCATGGGGGCAGATCTGAACAACATTGT; GAAATGTCAGAAGCTTACAGATGACCATGTTCAGTTCCTTATCTACCAAATTCTCCGAGGTCTAAAGTAT; ATACATTCAGCTGACATAATTCACAGGGACCTAAAACCTAGTAATCTAGCTGTGAATGAAGACTGTGAGC; TGAAGATTCTGGATTTTGGACTGGCTCGGCACACAGATGATGAAATGACAGGCTACGTGGCCACTAGGTG; GTACAGGGCTCCTGAGATCATGCTGAACTGGATGCATTACAACCAGACAGTTGATATTTGGTCAGTGGGA; TGCATAATGGCCGAGCTGTTGACTGGAAGAACATTGTTTCCTGGTACAGACCATATTGATCAGTTGAAGC; TCATTTTAAGACTCGTTGGAACCCCAGGGGCTGAGCTTTTGAAGAAAATCTCCTCAGAGTCTCTGTCGAC; TTGCTGGAGAAGATGCTTGTATTGGACTCAGATAAGAGAATTACAGCGGCCCAAGCCCTTGCACATGCCT; ACTTTGCTCAGTACCACGATCCTGATGATGAACCAGTGGCCGATCCTTATGATCAGTCCTTTGAAAGCAG; GGACCTCCTTATAG; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_139014 unedited; ACCGCCGTTCAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCATTTAGGTGA; CACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCGAATTCGGCACGAGGGCCCCACAGGGCC; ACCTTCTTGCCCGGCGGCTGCCGCTGGAAAATGTCTCAGGAGAGGCCCACGTTCTACCGGCAGGAGCTGA; ACAAGACAATCTGGGAGGTGCCCGAGCGTTACCAGAACCTGTCTCCAGTGGGCTCTGGCGCCTATGGCTC; TGTGTGTGCTGCTTTTTGACACAAAAAACGGGGGTTACGTGGTGGGCAGTGATGAACCTCTCCCAGACCA; TTTGTAGTTCATTCATTTCATGCCAAAAAGAACCCTACAGAGAAACTGCGGTAACTTAACCTAAGGAAAA; CATGAAATGTGGAATTGGCCGGGTTGAAACGTTTTTACCCCTGCAAGGTCCTGGGAGGGAATTATGGGAT; GTGTTCTGGGGGGCCATCTCTAAGGGGGGCAAACTGAAACCAAATTTGGAAAGTGTCAAACCTTAACAAA; AAAACCGGTTTATTTTCTTTTTTACCCAAATTTCCCAGGGGTTAAAGTTTTACACTTTTCCCTAAAAAAT; TTTACAGGGACCCTAACCCCGAAAACCCCCCCGGAAGAAGAAAACGGGAGCCAAAAAATCGGGATTTTGA; AGGGGGGGGGCAAAAAAAATTAGTTTTTTGAACCCCTTTTTAAACCTTGGGGGCCCCGGGGGGCGACTTT; TGAAAAAACACCTCTAAAATCGTGAAAAATATTTATCTTTTTTCCCCTGCCCAAAAAACTTTTGAAGATG; TATTTGTGGCGCACACCCGCGGTCGCTTCTGGGGAGAGATGTGTTTTGCTCCTACAAAATATATAGCGCC; GCCCTCGCTCGCATATGTCTCCACACACTGTGAAGAGCAGCGCGTCTTTATTCTTCTTTAAAGGAGCGCT; ATT;
  Kinase Domain Sequence

  >SC323402 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation; AWTKTTGMGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCATTTAGGTGACAC; TATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCGAATTCGGCACGAGGGCCCCACAGGGCCACC; TTCTTGCCCGGCGGCTGCCGCTGGAAAATGTCTCAGGAGAGGCCCACGTTCTACCGGCAGGAGCTGAACA; AGACAATCTGGGAGGTGCCCGAGCGTTACCAGAACCTGTCTCCAG;
• Restriction Sites: Please inquire
• ACCN: NM_139014
• Insert Size: 4700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_139014.1, NP_620583.1
• RefSeq Size: 3679 bp
• RefSeq ORF: 924 bp
• Locus ID: 1432
• UniProt ID: Q16539
• Cytogenetics: 6p21.31
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Amyotrophic lateral sclerosis (ALS), Epithelial cell signaling in Helicobacter pylori infection, Fc epsilon RI signaling pathway, GnRH signaling pathway, Leukocyte transendothelial migration, MAPK signaling pathway, Neurotrophin signaling pathway, NOD-like receptor signaling pathway, Progesterone-mediated oocyte maturation, RIG-I-like receptor signaling pathway, T cell receptor signaling pathway, Toll-like receptor signaling pathway, VEGF signaling pathway
• Summary: The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]; Transcript Variant: This variant (4) contains a different internal segment when compared to variant 1. It thus encodes an isoform that has a different and shorter internal segment, as compared to isoform 1.