PLK2 (NM_006622) Human Untagged Clone

CAT#: SC323362

PLK2 (untagged)-Kinase deficient mutant (K111M) of Human polo-like kinase 2 (Drosophila) (PLK2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PLK2
• Synonyms: hPlk2; hSNK; SNK
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC323362 sequence for NM_006622 edited (data generated by NextGen Sequencing)
  ATGGAGCTTTTGCGGACTATCACCTACCAGCCAGCCGCCAGCACCAAAATGTGCGAGCAG
  GCGCTGGGCAAGGGTTGCGGAGCGGACTCGAAGAAGAAGCGGCCGCCGCAGCCCCCCGAG
  GAATCGCAGCCACCTCAGTCCCAGGCGCAAGTGCCCCCGGCGGCCCCTCACCACCATCAC
  CACCATTCGCACTCGGGGCCGGAGATCTCGCGGATTATCGTCGACCCCACGACTGGGAAG
  CGCTACTGCCGGGGCAAAGTGCTGGGAAAGGGTGGCTTTGCAAAATGTTACGAGATGACA
  GATTTGACAAATAACAAAGTCTACGCCGCAATGATTATTCCTCACAGCAGAGTAGCTAAA
  CCTCATCAAAGGGAAAAGATTGACAAAGAAATAGAGCTTCACAGAATTCTTCATCATAAG
  CATGTAGTGCAGTTTTACCACTACTTCGAGGACAAAGAAAACATTTACATTCTCTTGGAA
  TACTGCAGTAGAAGGTCAATGGCTCATATTTTGAAAGCAAGAAAGGTGTTGACAGAGCCA
  GAAGTTCGATACTACCTCAGGCAGATTGTGTCTGGACTGAAATACCTTCATGAACAAGAA
  ATCTTGCACAGAGATCTCAAACTAGGGAACTTTTTTATTAATGAAGCCATGGAACTAAAA
  GTTGGGGACTTCGGTCTGGCAGCCAGGCTAGAACCCTTGGAACACAGAAGGAGAACGATA
  TGTGGTACCCCAAATTATCTCTCTCCTGAAGTCCTCAACAAACAAGGACATGGCTGTGAA
  TCAGACATTTGGGCCCTGGGCTGTGTAATGTATACAATGTTACTAGGGAGGCCCCCATTT
  GAAACTACAAATCTCAAAGAAACTTACAGGTGCATAAGGGAAGCAAGGTATACAATGCCA
  TCCTCATTGCTGGCTCCTGCCAAGCACTTAATTGCTAGTATGTTGTCCAAAAACCCAGAG
  GATCGTCCCAGTTTGGATGACATCATTCGACATGACTTTTTTTTGCAGGGCTTCACTCCG
  GACAGACTGTCTTCTAGCTGTTGTCATACAGTTCCAGATTTCCACTTATCAAGCCCAGCT
  AAGAATTTCTTTAAGAAAGCAGCTGCTGCTCTTTTTGGTGGCAAAAAAGACAAAGCAAGA
  TATATTGACACACATAATAGAGTGTCTAAAGAAGATGAAGACATCTACAAGCTTAGGCAT
  GATTTGAAAAAGACTTCAATAACTCAGCAACCCAGCAAACACAGGACAGATGAGGAGCTC
  CAGCCACCTACCACCACAGTTGCCAGGTCTGGAACACCCGCAGTAGAAAACAAGCAGCAG
  ATTGGGGATGCTATTCGGATGATAGTCAGAGGGACTCTTGGCAGCTGTAGCAGCAGCAGT
  GAATGCCTTGAAGACAGTACCATGGGAAGTGTTGCAGACACAGTGGCAAGGGTTCTTCGG
  GGATGTCTGGAAAACATGCCGGAAGCTGATTGCATTCCCAAAGAGCAGCTGAGCACATCA
  TTTCAGTGGGTCACCAAATGGGTTGATTACTCTAACAAATATGGCTTCGGGTACCAGCTC
  TCAGACCACACCGTCGGTGTCCTTTTCAACAATGGTGCTCACATGAGCCTCCTTCCAGAC
  AAAAAAACAGTTCACTATTACGCAGAGCTTGGCCAATGCTCAGTTTTCCCAGCAACAGAT
  GCTCCTGAGCAATTTATTAGTCAAGTGACGGTGCTGAAATACTTTTCTCATTACATGGAG
  GAGAACCTCATGGATGGTGGAGATCTGCCTAGTGTTACTGATATTCGAAGACCTCGGCTC
  TACCTCCTTCAGTGGCTAAAATCTGATAAGGCCCTAATGATGCTCTTTAATGATGGCACC
  TTTCAGGTGAATTTCTACCATGATCATACAAAAATCATCATCTGTAGCCAAAATGAAGAA
  TACCTTCTCACCTACATCAATGAGGATAGGATATCTACAACTTTCAGGCTGACAACTCTG
  CTGATGTCTGGCTGTTCATCAGAATTAAAAAATCGAATGGAATATGCCCTGAACATGCTC
  TTACAAAGATGTAACTGA
  
  Clone variation with respect to NM_006622.2
  332 a=>t;333 a=>g;867 t=>c;900 g=>a;1548 t=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_006622 unedited
  CCCGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAA
  CCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCGGGGTGTTGGGT
  GCTAGTCGGCACCAGAGGCAAGGGTGCGAGGACCACGGCCGGCTCGGACGTGTGACCGCGCCTAGGGGGT
  GGCAGCGGGCAGTGCGGGGCGGCAAGGCGACCATGGAGCTTTTGCGGACTATCACCTACCAGCCAGCCGC
  CAGCACCAAAATGTGCGAGCAGGCGCTGGGCAAGGGTTGCGGAGCGGACTCGAAGAAGAAGCGGGTCGCT
  GTCGGCCCCCCGAGGATTCGCAGCCCACCTCAGTCCCAGGCGCAGGTGCCCCCGGCGGCCCCTCACCACC
  ATCACCACCATTCGCACTCGGGGCCGGAAATCTCGCGGATTATCGTCGACCCACGACTGGGAGCCGCTAC
  TGCCGGGGCAAAGTGCTGGGAAGGGTTGGCTTTGCAAATGGTTACGAAAAGCAGGATTGCCAATACAAAG
  CCAGCCCCATTAATATTCCTAACGAGATAGTTAACCCTATCAAGGGAAAGATGCAAGAATAGCCTCACAG
  AATCTCTCACAGCAGTGAGCGAGTTTCCCTACTCAGAGACAAGAACATTACTCCTGGATCGCGATAAGTC
  AGTGCTAATTGACGAAGGTTCAGACCAATCTATCCTCAGCATTGTGGCAGACCTGACGAACTGCGATTCC
  ATGGACTTCTATGACGGCATGGACCCCCGCGCGCACTGACACGACAAGTGGCCTGC
  
  Kinase domain sequence

  >SC323362 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  GKWSSRRRSMWTCGTGCGMGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGT
  TTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCGGGG
  TGTTGGGTGCTAGTCGGCACCAGAGGCAAGGGTGCGAGGACCACGGCCGGCTCGGACGTGTGACCGCGCC
  TAGGGGGTGGCAGCGGGCAGTGCGGGGCGGCAAGGCGACCATGGA
  
• Restriction Sites: Please inquire
• ACCN: NM_006622
• Insert Size: 2690 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006622.1, NP_006613.1
• RefSeq Size: 2972 bp
• RefSeq ORF: 2058 bp
• Locus ID: 10769
• UniProt ID: Q9NYY3
• Cytogenetics: 5q11.2
• Domains: pkinase, POLO_box, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: The protein encoded by this gene is a member of the polo family of serine/threonine protein kinases that have a role in normal cell division. This gene is most abundantly expressed in testis, spleen and fetal tissues, and its expression is inducible by serum, suggesting that it may also play an important role in cells undergoing rapid cell division. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]
  Transcript Variant: This variant (1) represents the predominant transcript, and encodes the longer isoform (1).