SPARCL1 (NM_001128310) Human Untagged Clone

CAT#: SC323171

SPARCL1 (untagged)-Human SPARC-like 1 (hevin) (SPARCL1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SPARCL1
• Synonyms: MAST 9; MAST9; PIG33; SC1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001128310, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAAGACTGGGCTTTTTTTCCTATGTCTCTTGGGAACTGCAGCTGCAATCCCGACAAATGCAAGATTAT
  TATCTGATCATTCCAAACCAACTGCTGAAACGGTAGCACCTGACAACACTGCAATCCCCAGTTTAAGGGC
  TGAAGCTGAAGAAAATGAAAAAGAAACAGCAGTATCCACAGAAGACGATTCCCACCATAAGGCTGAAAAA
  TCATCAGTACTAAAGTCAAAAGAGGAAAGCCATGAACAGTCAGCAGAACAGGGCAAGAGTTCTAGCCAAG
  AGCTGGGATTGAAGGATCAAGAGGACAGTGATGGTCACTTAAGTGTGAATTTGGAGTATGCACCAACTGA
  AGGTACATTGGACATAAAAGAAGATATGAGTGAGCCTCAGGAGAAAAAACTCTCAGAGAACACTGATTTT
  TTGGCTCCTGGTGTTAGTTCCTTCACAGATTCTAACCAACAAGAAAGTATCACAAAGAGAGAGGAAAACC
  AAGAACAACCTAGAAATTATTCACATCATCAGTTGAACAGGAGCAGTAAACATAGCCAAGGCCTAAGGGA
  TCAAGGAAACCAAGAGCAGGATCCAAATATTTCCAATGGAGAAGAGGAAGAAGAAAAAGAGCCAGGTGAA
  GTTGGTACCCACAATGATAACCAAGAAAGAAAGACAGAATTGCCCAGGGAGCATGCTAACAGCAAGCAGG
  AGGAAGACAATACCCAATCTGATGATATTTTGGAAGAGTCTGATCAACCAACTCAAGTAAGCAAGATGCA
  GGAGGATGAATTTGATCAGGGTAACCAAGAACAAGAAGATAACTCCAATGCAGAAATGGAAGAGGAAAAT
  GCATCGAACGTCAATAAGCACATTCAAGAAACTGAATGGCAGAGTCAAGAGGGTAAAACTGGCCTAGAAG
  CTATCAGCAACCACAAAGAGACAGAAGAAAAGACTGTTTCTGAGGCTCTGCTCATGGAACCTACTGATGA
  TGGTAATACCACGCCCAGAAATCATGGAGTTGATGATGATGGCGATGATGATGGCGATGATGGCGGCACT
  GATGGCCCCAGGCACAGTGCAAGTGATGACTACTTCATCCCAAGCCAGGCCTTTCTGGAGGCCGAGAGAG
  CTCAATCCATTGCCTATCACCTCAAAATTGAGGAGCAAAGAGAAAAAGTACATGAAAATGAAAATATAGG
  TACCACTGAGCCTGGAGAGCACCAAGAGGCCAAGAAAGCAGAGAACTCATCAAATGAGGAGGAAACGTCA
  AGTGAAGGCAACATGAGGGTGCATGCTGTGGATTCTTGCATGAGCTTCCAGTGTAAAAGAGGCCACATCT
  GTAAGGCAGACCAACAGGGAAAACCTCACTGTGTCTGCCAGGATCCAGTGACTTGTCCTCCAACAAAACC
  CCTTGATCAAGTTTGTGGCACTGACAATCAGACCTATGCTAGTTCCTGTCATCTATTCGCTACTAAATGC
  AGACTGGAGGGGACCAAAAAGGGGCATCAACTCCAGCTGGATTATTTTGGAGCCTGCAAATCTATTCCTA
  CTTGTACGGACTTTGAAGTGATTCAGTTTCCTCTACGGATGAGAGACTGGCTCAAGAATATCCTCATGCA
  GCTTTATGAAGCCAACTCTGAACACGCTGGTTATCTAAATGAGAAGCAGAGAAATAAAGTCAAGAAAATT
  TACCTGGATGAAAAGAGGCTTTTGGCTGGGGACCATCCCATTGATCTTCTCTTAAGGGACTTTAAGAAAA
  ACTACCACATGTATGTGTATCCTGTGCACTGGCAGTTTAGTGAACTTGACCAACACCCTATGGATAGAGT
  CTTGACACATTCTGAACTTGCTCCTCTGCGAGCATCTCTGGTGCCCATGGAACACTGCATAACCCGTTTC
  TTTGAGGAGTGTGACCCCAACAAGGATAAGCACATCACCCTGAAGGAGTGGGGCCACTGCTTTGGAATTA
  AAGAAGAGGACATAGATGAAAATCTCTTGTTTTGA
  
  
• Restriction Sites: Please inquire
• ACCN: NM_001128310
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001128310.1, NP_001121782.1
• RefSeq Size: 3058 bp
• RefSeq ORF: 1995 bp
• Locus ID: 8404
• UniProt ID: Q14515
• Cytogenetics: 4q22.1
• Protein Families: Druggable Genome, Secreted Protein