OLFM2 (NM_058164) Human Untagged Clone

CAT#: SC322272

OLFM2 (untagged)-Human olfactomedin 2 (OLFM2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: OLFM2
• Synonyms: NOE2; NOELIN2; NOELIN2_V1; OlfC
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for SC322272 CTAGGTCGGGACGCCGGGCTAGGGGCGCGTCATGTGGCCGCTCACGGTCCCGCCGCCGCT
  GCTGCTGCTGCTGTGCTCAGGCCTGGCCGGACAGACTCTCTTCCAGAACCCAGAAGAGGG
  CTGGCAGCTGTACACCTCAGCCCAGGCCCCTGACGGGAAATGCATCTGCACGGCCGTGAT
  CCCAGCGCAGAGTACCTGCTCTCGAGATGGCAGGAGTCGGGAGCTGCGGCAACTGATGGA
  GAAGGTCCAGAACGTCTCCCAGTCCATGGAGGTCCTTGAGTTGCGGACGTATCGCGACCT
  CCAGTATGTACGCGGCATGGAGACCCTCATGCGGAGCCTGGATGCGCGGCTCCGGGCAGC
  TGATGGGTCCCTCTCGGCCAAGAGCTTCCAGGAGCTGAAGGACAGGATGACGGAACTGTT
  GCCCCTGAGCTCGGTCCTGGAGCAGTACAAGGCAGACACGCGGACCATTGTACGCTTGCG
  GGAGGAGGTGAGGAATCTCTCCGGCAGTCTGGCGGCCATTCAGGAGGAGATGGGTGCCTA
  CGGGTATGAGGACCTGCAGCAACGGGTGATGGCCCTGGAGGCCCGGCTCCACGCCTGCGC
  CCAGAAGCTGGGCTGTGGGAAGCTGACCGGGGTCAGTAACCCCATCACCGTTCGGGCCAT
  GGGGTCCCGCTTCGGCTCCTGGATGACTGACACGATGGCCCCCAGTGCGGATAGCCGGGT
  CTGGTACATGGATGGCTATTACAAAGGCCGCCGGGTCCTGGAGTTCCGTACCCTGGGAGA
  CTTCATCAAAGGCCAGAACTTTATCCAGCACCTGCTGCCCCAGCCGTGGGCGGGCACGGG
  CCACGTGGTGTACAACGGCTCCCTGTTCTATAACAAGTACCAGAGCAACGTGGTGGTCAA
  ATACCACTTCCGCTCGCGCTCTGTGCTGGTGCAGAGGAGCCTCCCGGGCGCCGGTTACAA
  CAACACCTTCCCCTACTCCTGGGGCGGCTTCTCCGACATGGACTTCATGGTGGACGAGAG
  CGGGCTCTGGGCTGTGTACACCACCAACCAGAACGCGGGCAACATCGTGGTCAGCCGGCT
  GGACCCGCACACCCTCGAGGTCATGCGGTCCTGGGACACCGGCTACCCCAAGCGCAGCGC
  TGGCGAGGCCTTCATGATCTGCGGTGTGCTCTACGTGACCAACTCCCACCTGGCTGGGGC
  CAAGGTCTACTTCGCCTATTTTACCAACACGTCCAGTTACGAGTACACGGACGTGCCCTT
  CCACAACCAGTATTCCCACATCTCGATGCTGGATTACAACCCCCGGGAGCGCGCCCTCTA
  TACCTGGAACAACGGCCACCAGGTGCTCTACAATGTCACCCTGTTTCACGTCATCAGCAC
  CTCTGGGGACCCCTGAGCCAATGCTGTGGCTCGGGCTGCTGCCTGGGGGGCCTCCGGGGG
  CTGGGGGCCCTTTTCATTCTGCCTGTGTCCCTCAAGGGTGATCTCTCTGTCTCTGTCACG
  CCCTTTCTCCCCGCCTTTTTGCTGGGCTTTTGTTCTCTGCCTATGTATTTCTGTCTATTT
  TTTCAATTTCCCCTCTTCTCCTTTATTGATCTCTGCTTTTAATACACCACTTCTTTCTTT
  CTGCCTTTTTATGGATGTCTTTTTCTTTTTATGGCTCTGGTTCTCCAGTTCTTTCCGTCT
  CTGCCTCTCTCTGTCTCTCTCTCTCTGTCCTTCCACCCCTCCCTCCTTGCTTCCCACCCA
  TTCCTCATCCCTCACTCCCACCCCCACCCCCACCCCCAGGAGTTGAGTGCATGGATCTGT
  TTCTTTTTTTATTTACACTTTTTCTTTCCGGTTTGCCGGAATAAACAGGACCTTTGACAT
  TTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_058164
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_058164.1, NP_477512.1
• RefSeq Size: 1902 bp
• RefSeq ORF: 1365 bp
• Locus ID: 93145
• UniProt ID: O95897
• Cytogenetics: 19p13.2
• Domains: OLF
• Protein Families: Druggable Genome, Secreted Protein
• Gene Summary: Involved in transforming growth factor beta (TGF-beta)-induced smooth muscle differentiation. TGF-beta induces expression and translocation of OLFM2 to the nucleus where it binds to SRF, causing its dissociation from the transcriptional repressor HEY2/HERP1 and facilitating binding of SRF to target genes (PubMed:25298399). Plays a role in AMPAR complex organization (By similarity). Is a regulator of vascular smooth-muscle cell (SMC) phenotypic switching, that acts by promoting RUNX2 and inhibiting MYOCD binding to SRF. SMC phenotypic switching is the process through which vascular SMCs undergo transition between a quiescent contractile phenotype and a proliferative synthetic phenotype in response to pathological stimuli. SMC phenotypic plasticity is essential for vascular development and remodeling (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) uses an alternate 5' terminal exon, lacks a portion of the 5' coding region, and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (2) has a distinct N-terminus and is shorter than isoform 1.