TRAIL (TNFSF10) (NM_003810) Human Untagged Clone

CAT#: SC321920

TNFSF10 (untagged)-Human tumor necrosis factor (ligand) superfamily, member 10 (TNFSF10), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TRAIL
• Synonyms: Apo-2L; APO2L; CD253; TL2; TNLG6A; TRAIL
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_003810.2 CCACGCGTCCGAGAAGGAAGGGCTTCAGTGACCGGCTGCCTGGCTGACTTACAGCAGTCA
  GACTCTGACAGGATCATGGCTATGATGGAGGTCCAGGGGGGACCCAGCCTGGGACAGACC
  TGCGTGCTGATCGTGATCTTCACAGTGCTCCTGCAGTCTCTCTGTGTGGCTGTAACTTAC
  GTGTACTTTACCAACGAGCTGAAGCAGATGCAGGACAAGTACTCCAAAAGTGGCATTGCT
  TGTTTCTTAAAAGAAGATGACAGTTATTGGGACCCCAATGACGAAGAGAGTATGAACAGC
  CCCTGCTGGCAAGTCAAGTGGCAACTCCGTCAGCTCGTTAGAAAGATGATTTTGAGAACC
  TCTGAGGAAACCATTTCTACAGTTCAAGAAAAGCAACAAAATATTTCTCCCCTAGTGAGA
  GAAAGAGGTCCTCAGAGAGTAGCAGCTCACATAACTGGGACCAGAGGAAGAAGCAACACA
  TTGTCTTCTCCAAACTCCAAGAATGAAAAGGCTCTGGGCCGCAAAATAAACTCCTGGGAA
  TCATCAAGGAGTGGGCATTCATTCCTGAGCAACTTGCACTTGAGGAATGGTGAACTGGTC
  ATCCATGAAAAAGGGTTTTACTACATCTATTCCCAAACATACTTTCGATTTCAGGAGGAA
  ATAAAAGAAAACACAAAGAACGACAAACAAATGGTCCAATATATTTACAAATACACAAGT
  TATCCTGACCCTATATTGTTGATGAAAAGTGCTAGAAATAGTTGTTGGTCTAAAGATGCA
  GAATATGGACTCTATTCCATCTATCAAGGGGGAATATTTGAGCTTAAGGAAAATGACAGA
  ATTTTTGTTTCTGTAACAAATGAGCACTTGATAGACATGGACCATGAAGCCAGTTTTTTC
  GGGGCCTTTTTAGTTGGCTAACTGACCTGGAAAGAAAAAGCAATAACCTCAAAGTGACTA
  TTCAGTTTTCAGGATGATACACTATGAAGATGTTTCAAAAAATCTGACCAAAACAAACAA
  ACAGAAAACAGAAAACAAAAAAACCTCTATGCAATCTGAGTAGAGCAGCCACAACCAAAA
  AATTCTACAACACACACTGTTCTGAAAGTGACTCACTTATCCCAAGAAAATGAAATTGCT
  GAAAGATCTTTCAGGACTCTACCTCATATCAGTTTGCTAGCAGAAATCTAGAAGACTGTC
  AGCTTCCAAACATTAATGCAATGGTTAACATCTTCTGTCTTTATAATCTACTCCTTGTAA
  AGACTGTAGAAGAAAGCGCAACAATCCATCTCTCAAGTAGTGTATCACAGTAGTAGCCTC
  CAGGTTTCCTTAAGGGACAACATCCTTAAGTCAAAAGAGAGAAGAGGCACCACTAAAAGA
  TCGCAGTTTGCCTGGTGCAGTGGCTCACACCTGTAATCCCAACATTTTGGGAACCCAAGG
  TGGGTAGATCACGAGATCAAGAGATCAAGACCATAGTGACCAACATAGTGAAACCCCATC
  TCTACTGAAAGTGCAAAAATTAGCTGGGTGTGTTGGCACATGCCTGTAGTCCCAGCTACT
  TGAGAGGCTGAGGCAGGAGAATCGTTTGAACCCGGGAGGCAGAGGTTGCAGTGTGGTGAG
  ATCATGCCACTACACTCCAGCCTGGCGACAGAGCGAGACTTGGTTTCAAAAAAAAAAAAA
  AAAAAAACTTCAGTAAGTACGTGTTATTTTTTTCAATAAAATTCTATTACAGTAAAAAAA
  AAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_003810
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003810.2, NP_003801.1
• RefSeq Size: 1776 bp
• RefSeq ORF: 846 bp
• Locus ID: 8743
• UniProt ID: P50591
• Cytogenetics: 3q26.31
• Domains: TNF
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Apoptosis, Cytokine-cytokine receptor interaction, Natural killer cell mediated cytotoxicity
• Gene Summary: The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This protein preferentially induces apoptosis in transformed and tumor cells, but does not appear to kill normal cells although it is expressed at a significant level in most normal tissues. This protein binds to several members of TNF receptor superfamily including TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and possibly also to TNFRSF11B/OPG. The activity of this protein may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and TNFRSF11B/OPG that cannot induce apoptosis. The binding of this protein to its receptors has been shown to trigger the activation of MAPK8/JNK, caspase 8, and caspase 3. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2010]
  Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.