GALNT6 (NM_007210) Human Untagged Clone

CAT#: SC321908

GALNT6 (untagged)-Human UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 6 (GalNAc-T6) (GALNT6)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GALNT6
• Synonyms: GALNAC-T6; GalNAcT6
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_007210.3 CCACGCGTCCGGTCCCTCCTGCCTTCGCTCTCTGCTGGTCGCGGTGGCCTGTAGCTCCTC
  CCTCGGTAGGACCCCCCTCCACCTTGGCGCTTACGAGATGAATGAGGAGCCCAGACCTCT
  GGAACTTGGAGGGTTGTTCAGATTCCCAGGCTGACAAAAAGGAGAAACAGCTTTTACTTG
  CATCCAGGGCCAATCATCGCAGACCCAGACGTCTGCAGAGGGCTGAGGCCCCAGCTTGTG
  GCCACCACAACGTATCAAGCTATCTCCAGGGTTGGGCTCAGGACTCAGAGCTGACGCAGC
  TGGGGTGCCCCTTGGTTCTGGAGGATGAGGCTCCTCCGCAGACGCCACATGCCCCTGCGC
  CTGGCCATGGTGGGCTGCGCCTTTGTGCTCTTCCTCTTCCTCCTGCATAGGGATGTGAGC
  AGCAGAGAGGAGGCCACAGAGAAGCCGTGGCTGAAGTCCCTGGTGAGCCGGAAGGATCAC
  GTCCTGGACCTCATGCTGGAGGCCATGAACAACCTTAGAGATTCAATGCCCAAGCTCCAA
  ATCAGGGCTCCAGAAGCCCAGCAGACTCTGTTCTCCATAAACCAGTCCTGCCTCCCTGGG
  TTCTATACCCCAGCTGAACTGAAGCCCTTCTGGGAACGGCCACCACAGGACCCCAATGCC
  CCTGGGGCAGATGGAAAAGCATTTCAGAAGAGCAAGTGGACCCCCCTGGAGACCCAGGAA
  AAGGAAGAAGGCTATAAGAAGCACTGTTTCAATGCCTTTGCCAGCGACCGGATCTCCCTG
  CAGAGGTCCCTGGGGCCAGACACCCGACCACCTGAGTGTGTGGACCAGAAGTTCCGGCGC
  TGCCCCCCACTGGCCACCACCAGCGTGATCATTGTGTTCCACAACGAAGCCTGGTCCACA
  CTGCTGCGAACAGTGTACAGCGTCCTACACACCACCCCTGCCATCTTGCTCAAGGAGATC
  ATACTGGTGGATGATGCCAGCACAGAGGAGCACCTAAAGGAGAAGCTGGAGCAGTACGTG
  AAGCAGCTGCAGGTGGTGAGGGTGGTGCGGCAGGAGGAGCGGAAGGGGCTGATCACCGCC
  CGGCTGCTGGGGGCCAGCGTGGCACAGGCGGAGGTGCTCACGTTCCTGGATGCCCACTGT
  GAGTGCTTCCACGGCTGGCTGGAGCCCCTCCTGGCTCGAATCGCTGAGGACAAGACAGTG
  GTGGTGAGCCCAGACATCGTCACCATCGACCTTAATACTTTTGAGTTCGCCAAGCCCGTC
  CAGAGGGGCAGAGTCCATAGCCGAGGCAACTTTGACTGGAGCCTGACCTTCGGCTGGGAA
  ACACTTCCTCCACATGAGAAGCAGAGGCGCAAGGATGAAACCTACCCCATCAAATCCCCG
  ACGTTTGCTGGTGGCCTCTTCTCCATCTCCAAGTCCTACTTTGAGCACATCGGTACCTAT
  GATAATCAGATGGAGATCTGGGGAGGGGAGAACGTGGAAATGTCCTTCCGGGTGTGGCAG
  TGTGGGGGCCAGCTGGAGATCATCCCCTGCTCTGTCGTAGGCCATGTGTTCCGGACCAAG
  AGCCCCCACACCTTCCCCAAGGGCACTAGTGTCATTGCTCGCAATCAAGTGCGCCTGGCA
  GAGGTCTGGATGGACAGCTACAAGAAGATTTTCTATAGGAGAAATCTGCAGGCAGCAAAG
  ATGGCCCAAGAGAAATCCTTCGGTGACATTTCGGAACGACTGCAGCTGAGGGAACAACTG
  CACTGTCACAACTTTTCCTGGTACCTGCACAATGTCTACCCAGAGATGTTTGTTCCTGAC
  CTGACGCCCACCTTCTATGGTGCCATCAAGAACCTCGGCACCAACCAATGCCTGGATGTG
  GGTGAGAACAACCGCGGGGGGAAGCCCCTCATCATGTACTCCTGCCACGGCCTTGGCGGC
  AACCAGTACTTTGAGTACACAACTCAGAGGGACCTTCGCCACAACATCGCAAAGCAGCTG
  TGTCTACATGTCAGCAAGGGTGCTCTGGGCCTTGGGAGCTGTCACTTCACTGGCAAGAAT
  AGCCAGGTCCCCAAGGACGAGGAATGGGAATTGGCCCAGGATCAGCTCATCAGGAACTCA
  GGATCTGGTACCTGCCTGACATCCCAGGACAAAAAGCCAGCCATGGCCCCCTGCAATCCC
  AGTGACCCCCATCAGTTGTGGCTCTTTGTCTAGGACCCAGATCATCCCCAGAGAGAGCCC
  CCACAAGCTCCTCAGGAAACAGGATTGCTGATGTCTGGGAACCTGATCACCAGCTTCTCT
  GGAGGCCGTAAAGATGGATTTCTAAACCCACTGGGTGGCAAGGCAGGACCTTCCTAATCC
  TTGCAACAACATTGGGCCCATTTTCTTTCCTTCACACCGATGGAAGAGACCATTAGGACA
  TATATTTAGCCTAGCGTTTTCCTGTTCTAGAAATAGAGGCTCCCAAAGTAGGGAAGGCAG
  CTGGGGGAGGGTTCAGGGCAGCAATGCTGAGTTCAAGAAAAGTACTTCAGGCTGGGCACA
  GTGGCTCATGCCTGAAATCCTAGCACTTTGGGAAGACAATGTGGGAGAATGGCTTGAGCC
  CAGGAGTTCAAGACCGGCCTGAGCAACATAGTGAGGATCCCATCTCTACGCCCACCCTCC
  CCCCGGCAAAAAAAAAAGCTGGGTATGGTGGCTTATGCCTGTAGTCGCAGCTACTCAGAA
  GGCTGAGGTGGGAGGATTGCTTGTTCCCCGGAGGTTGAAGCTACAGTGAGCCTTGATTGT
  GTCACTGCACTCCAGCCTGGGCAACAGGTAAGACTCTGTCTCAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_007210
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007210.3, NP_009141.2
• RefSeq Size: 4520 bp
• RefSeq ORF: 1869 bp
• Locus ID: 11226
• UniProt ID: Q8NCL4
• Cytogenetics: 12q13.13
• Domains: RICIN, Glycos_transf_2
• Protein Families: Transmembrane
• Protein Pathways: Metabolic pathways, O-Glycan biosynthesis
• Gene Summary: This gene encodes a member of the UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase (GalNAc-T) family of enzymes. GalNAc-Ts initiate mucin-type O-linked glycosylation in the Golgi apparatus by catalyzing the transfer of GalNAc to serine and threonine residues on target proteins. They are characterized by an N-terminal transmembrane domain, a stem region, a lumenal catalytic domain containing a GT1 motif and Gal/GalNAc transferase motif, and a C-terminal ricin/lectin-like domain. GalNAc-Ts have different, but overlapping, substrate specificities and patterns of expression. The encoded protein is capable of glycosylating fibronectin peptide in vitro and is expressed in a fibroblast cell line, indicating that it may be involved in the synthesis of oncofetal fibronectin. [provided by RefSeq, Jul 2008]