ALDH1A1 (NM_000689) Human Untagged Clone

CAT#: SC321535

ALDH1A1 (untagged)-Human aldehyde dehydrogenase 1 family, member A1 (ALDH1A1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ALDH1A1
• Synonyms: ALDC; ALDH-E1; ALDH1; ALDH11; HEL-9; HEL-S-53e; HEL12; PUMB1; RALDH1
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000689.3 CTGTGTTCCAGGAGCCGAATCAGAAATGTCATCCTCAGGCACGCCAGACTTACCTGTCCT
  ACTCACCGATTTGAAGATTCAATATACTAAGATCTTCATAAACAATGAATGGCATGATTC
  AGTGAGTGGCAAGAAATTTCCTGTCTTTAATCCTGCAACTGAGGAGGAGCTCTGCCAGGT
  AGAAGAAGGAGATAAGGAGGATGTTGACAAGGCAGTGAAGGCCGCAAGACAGGCTTTTCA
  GATTGGATCCCCGTGGCGTACTATGGATGCTTCCGAGAGGGGGCGACTATTATACAAGTT
  GGCTGATTTAATCGAAAGAGATCGTCTGCTGCTGGCGACAATGGAGTCAATGAATGGTGG
  AAAACTCTATTCCAATGCATATCTGAATGATTTAGCAGGCTGCATCAAAACATTGCGCTA
  CTGTGCAGGTTGGGCTGACAAGATCCAGGGCCGTACAATACCAATTGATGGAAATTTTTT
  TACATATACAAGACATGAACCTATTGGTGTATGTGGCCAAATCATTCCTTGGAATTTCCC
  GTTGGTTATGCTCATTTGGAAGATAGGGCCTGCACTGAGCTGTGGAAACACAGTGGTTGT
  CAAACCAGCAGAGCAAACTCCTCTCACTGCTCTCCACGTGGCATCTTTAATAAAAGAGGC
  AGGGTTTCCTCCTGGAGTAGTGAATATTGTTCCTGGTTATGGGCCTACAGCAGGGGCAGC
  CATTTCTTCTCACATGGATATAGACAAAGTAGCCTTCACAGGATCAACAGAGGTTGGCAA
  GTTGATCAAAGAAGCTGCCGGGAAAAGCAATCTGAAGAGGGTGACCCTGGAGCTTGGAGG
  AAAGAGCCCTTGCATTGTGTTAGCTGATGCCGACTTGGACAATGCTGTTGAATTTGCACA
  CCATGGGGTATTCTACCACCAGGGCCAGTGTTGTATAGCCGCATCCAGGATTTTTGTGGA
  AGAATCAATTTATGATGAGTTTGTTCGAAGGAGTGTTGAGCGGGCTAAGAAGTATATCCT
  TGGAAATCCTCTGACCCCAGGAGTCACTCAAGGCCCTCAGATTGACAAGGAACAATATGA
  TAAAATACTTGACCTCATTGAGAGTGGGAAGAAAGAAGGGGCCAAACTGGAATGTGGAGG
  AGGCCCGTGGGGGAATAAAGGCTACTTTGTCCAGCCCACAGTGTTCTCTAATGTTACAGA
  TGAGATGCGCATTGCCAAAGAGGAGATTTTTGGACCAGTGCAGCAAATCATGAAGTTTAA
  ATCTTTAGATGACGTGATCAAAAGAGCAAACAATACTTTCTATGGCTTATCAGCAGGAGT
  GTTTACCAAAGACATTGATAAAGCCATAACAATCTCCTCTGCTCTGCAGGCAGGAACAGT
  GTGGGTGAATTGCTATGGCGTGGTAAGTGCCCAGTGCCCCTTTGGTGGATTCAAGATGTC
  TGGAAATGGAAGAGAACTGGGAGAGTACGGTTTCCATGAATATACAGAGGTCAAAACAGT
  CACAGTGAAAATCTCTCAGAAGAACTCATAAAGAAAATACAAGAGTGGAGAGAAGCTCTT
  CAATAGCTAAGCATCTCCTTACAGTCACTAATATAGTAGATTTTAAAGACAAAATTTTTC
  TTTTCTTGATTTTTTTAAACATAAGCTAAATCATATTAGTATTAATACTACCCATAGAAA
  ACTTGACATGTAGCTTCTTCTGAAAGAATTATTTGCCTTCTGAAATGTGACCCCCAAGTC
  CTATCCTAAATAAAAAAAGACAAATTCGGATGTATGATCTCTCTAGCTTTGTCATAGTTA
  TGTGATTTTCCTTTGTAGCTACTTTTGCAGGATAATAATTTTATAGAAAAGGAACAGTTG
  CATTTAGCTTCTTTCCCTTAGTGACTCTTGAAGTACTTAACATACACGTTAACTGTAGAG
  TAAATTGCTCTGTTCCCAGTAGTTATAAAGTCCTTGGACTGTTTTGAAAAGTTTCCTAGG
  ATGTCATGTCTGCTTGTCAAAAGAAATAATCCCTGTAATATTTAGCTGTAAACTGAATAT
  AAAGCTTAATAAAAACAACCTTGCATGAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_000689
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000689.3, NP_000680.2
• RefSeq Size: 2116 bp
• RefSeq ORF: 1506 bp
• Locus ID: 216
• UniProt ID: P00352
• Cytogenetics: 9q21.13
• Domains: aldedh
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS
• Protein Pathways: Metabolic pathways, Retinol metabolism
• Gene Summary: The protein encoded by this gene belongs to the aldehyde dehydrogenase family. Aldehyde dehydrogenase is the next enzyme after alcohol dehydrogenase in the major pathway of alcohol metabolism. There are two major aldehyde dehydrogenase isozymes in the liver, cytosolic and mitochondrial, which are encoded by distinct genes, and can be distinguished by their electrophoretic mobility, kinetic properties, and subcellular localization. This gene encodes the cytosolic isozyme. Studies in mice show that through its role in retinol metabolism, this gene may also be involved in the regulation of the metabolic responses to high-fat diet. [provided by RefSeq, Mar 2011]