xCT (SLC7A11) (NM_014331) Human Untagged Clone

CAT#: SC321449

SLC7A11 (untagged)-Human solute carrier family 7, (cationic amino acid transporter, y+ system) member 11 (SLC7A11)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: xCT
• Synonyms: CCBR1; xCT
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_014331.3 GAAAAAGCACACCTTTGAGTTTTCACCTGTGAACACTATAGCGCTGACAGAGACAGTCTG
  AAAGCAGAGGAAGACATCGATCAGTAACACCAAGAGACACCAAAGTTGAAAGTTTTGTTT
  TCTTTCCCTCTGTTTTATTTTTCCCCCGTGTGTCCCTACTATGGTCAGAAAGCCTGTTGT
  GTCCACCATCTCCAAAGGAGGTTACCTGCAGGGAAATGTTAACGGGAGGCTGCCTTCCCT
  GGGCAACAAGGAGCCACCTGGGCAGGAGAAAGTGCAGCTGAAGAGGAAAGTCACTTTACT
  GAGGGGAGTCTCCATTATCATTGGCACCATCATTGGAGCAGGAATCTTCATCTCTCCTAA
  GGGCGTGCTCCAGAACACGGGCAGCGTGGGCATGTCTCTGACCATCTGGACGGTGTGTGG
  GGTCCTGTCACTATTTGGAGCTTTGTCTTATGCTGAATTGGGAACAACTATAAAGAAATC
  TGGAGGTCATTACACATATATTTTGGAAGTCTTTGGTCCATTACCAGCTTTTGTACGAGT
  CTGGGTGGAACTCCTCATAATACGCCCTGCAGCTACTGCTGTGATATCCCTGGCATTTGG
  ACGCTACATTCTGGAACCATTTTTTATTCAATGTGAAATCCCTGAACTTGCGATCAAGCT
  CATTACAGCTGTGGGCATAACTGTAGTGATGGTCCTAAATAGCATGAGTGTCAGCTGGAG
  CGCCCGGATCCAGATTTTCTTAACCTTTTGCAAGCTCACAGCAATTCTGATAATTATAGT
  CCCTGGAGTTATGCAGCTAATTAAAGGTCAAACGCAGAACTTTAAAGACGCCTTTTCAGG
  AAGAGATTCAAGTATTACGCGGTTGCCACTGGCTTTTTATTATGGAATGTATGCATATGC
  TGGCTGGTTTTACCTCAACTTTGTTACTGAAGAAGTAGAAAACCCTGAAAAAACCATTCC
  CCTTGCAATATGTATATCCATGGCCATTGTCACCATTGGCTATGTGCTGACAAATGTGGC
  CTACTTTACGACCATTAATGCTGAGGAGCTGCTGCTTTCAAATGCAGTGGCAGTGACCTT
  TTCTGAGCGGCTACTGGGAAATTTCTCATTAGCAGTTCCGATCTTTGTTGCCCTCTCCTG
  CTTTGGCTCCATGAACGGTGGTGTGTTTGCTGTCTCCAGGTTATTCTATGTTGCGTCTCG
  AGAGGGTCACCTTCCAGAAATCCTCTCCATGATTCATGTCCGCAAGCACACTCCTCTACC
  AGCTGTTATTGTTTTGCACCCTTTGACAATGATAATGCTCTTCTCTGGAGACCTCGACAG
  TCTTTTGAATTTCCTCAGTTTTGCCAGGTGGCTTTTTATTGGGCTGGCAGTTGCTGGGCT
  GATTTATCTTCGATACAAATGCCCAGATATGCATCGTCCTTTCAAGGTGCCACTGTTCAT
  CCCAGCTTTGTTTTCCTTCACATGCCTCTTCATGGTTGCCCTTTCCCTCTATTCGGACCC
  ATTTAGTACAGGGATTGGCTTCGTCATCACTCTGACTGGAGTCCCTGCGTATTATCTCTT
  TATTATATGGGACAAGAAACCCAGGTGGTTTAGAATAATGTCAGAGAAAATAACCAGAAC
  ATTACAAATAATACTGGAAGTTGTACCAGAAGAAGATAAGTTATGAACTAATGGACTTGA
  GATCTTGGCAATCTGCCCAAGGGGAGACACAAAATAGGGATTTTTACTTCATTTTCTGAA
  AGTCTAGAGAATTACAACTTTGGTGATAAACAAAAGGAGTCAGTTATTTTTATTCATATA
  TTTTATCATATTCGAACTAATTTCTAAGAAATTTAGTTATAACTCTATGTAGTTATAGAA
  AGTGAATATGCAGTTATTCTATGATTCGCACAATTCTTGAGTCTCTGATACCTACCTATT
  GGGGTTAGGAGAAAAGACTAGACAATTACTATGTGGTCATTCTCTACAACATATGTTAGC
  ACGGCAAAGAACCTTCAAATTGAAGACTGAGATTTTTCTGTATATATGGGTTTTGTAAAG
  ATGGTTTTACACACTATAGATGTCTATACTGTGAAAAGTGTTTTCAATTCTGAAAAAAAG
  CATACATCATGATTATGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_014331
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014331.3, NP_055146.1
• RefSeq Size: 9648 bp
• RefSeq ORF: 1506 bp
• Locus ID: 23657
• UniProt ID: Q9UPY5
• Cytogenetics: 4q28.3
• Domains: aa_permeases
• Protein Families: Druggable Genome, Transmembrane
• Gene Summary: This gene encodes a member of a heteromeric, sodium-independent, anionic amino acid transport system that is highly specific for cysteine and glutamate. In this system, designated Xc(-), the anionic form of cysteine is transported in exchange for glutamate. This protein has been identified as the predominant mediator of Kaposi sarcoma-associated herpesvirus fusion and entry permissiveness into cells. Also, increased expression of this gene in primary gliomas (compared to normal brain tissue) was associated with increased glutamate secretion via the XCT channels, resulting in neuronal cell death. [provided by RefSeq, Sep 2011]