STK25 (NM_006374) Human Untagged Clone

CAT#: SC321266

STK25 (untagged)-Human serine/threonine kinase 25 (STK25)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: STK25
• Synonyms: SOK1; YSK1
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_006374.3 GCGACGCCGAGCACCGCCCTCGCCGTCGCCTCCGGGCTTTCTCCGGTCGCTGCCGCCACC
  ACCGTTGCTTCGCGGGCTGGGAGGCCCGGGGTCCCCGGGCGAACAGAGGCTGCGGTGGAC
  TGACGCCGCAGGGGCGAGCTAGCCGGCTCCGCGCCTCTCCGCGGGATCCAGACGCCTCCT
  GGGGCTGCTGGCGGAGGGTCTGAGGCGGCGCGGCCATGGCTCACCTCCGGGGATTTGCCA
  ACCAGCACTCTCGAGTGGACCCTGAGGAGCTCTTCACCAAGCTCGACCGCATTGGCAAGG
  GCTCGTTTGGGGAGGTCTACAAGGGCATCGATAACCACACAAAGGAGGTGGTGGCCATCA
  AGATCATCGACCTGGAGGAGGCCGAGGATGAGATCGAGGACATCCAGCAGGAGATCACTG
  TCCTCAGTCAGTGCGACAGCCCCTACATCACCCGCTACTTTGGCTCCTACCTAAAGAGCA
  CCAAGCTATGGATCATCATGGAGTACCTGGGCGGCGGCTCAGCACTGGACTTGCTTAAAC
  CAGGTCCCCTGGAGGAGACATACATTGCCACGATCCTGCGGGAGATTCTGAAGGGCCTGG
  ATTATCTGCACTCCGAACGCAAGATCCACCGAGACATCAAAGCTGCCAACGTGCTACTCT
  CGGAGCAGGGTGACGTGAAGCTGGCGGACTTTGGGGTAGCAGGGCAGCTCACAGACACGC
  AGATTAAGAGGAACACATTCGTGGGCACCCCCTTCTGGATGGCACCTGAGGTCATCAAGC
  AGTCGGCCTACGACTTCAAGGCTGACATCTGGTCCCTGGGGATCACAGCCATCGAGCTGG
  CCAAGGGGGAGCCTCCAAACTCTGACCTCCACCCCATGCGCGTCCTGTTCCTGATTCCCA
  AGAACAGCCCACCCACACTGGAGGGCCAGCACAGCAAGCCCTTCAAGGAGTTCGTGGAGG
  CCTGCCTCAACAAAGACCCCCGATTCCGGCCCACGGCCAAGGAGCTCCTGAAGCACAAGT
  TCATCACACGCTACACCAAGAAGACCTCCTTCCTCACGGAGCTCATCGACCGCTATAAGC
  GCTGGAAGTCAGAGGGGCATGGCGAGGAGTCCAGCTCTGAGGACTCTGACATTGATGGCG
  AGGCGGAGGACGGGGAGCAGGGCCCCATCTGGACGTTCCCCCCTACCATCCGGCCGAGTC
  CACACAGCAAGCTTCACAAGGGGACGGCCCTGCACAGTTCACAGAAGCCTGCGGAGCCCG
  TCAAGAGGCAGCCGAGGTCCCAGTGCCTGTCCACGCTGGTCCGGCCCGTCTTCGGAGAGC
  TCAAAGAGAAGCACAAGCAGAGCGGCGGGAGCGTGGGTGCGCTGGAGGAGCTGGAGAACG
  CCTTCAGCCTGGCCGAGGAGTCCTGCCCCGGCATCTCAGACAAGCTGATGGTGCACCTGG
  TGGAGCGAGTGCAGAGGTTTTCACACAACAGAAACCACCTGACATCCACCCGCTGAAGCG
  CACTGCTGTTCAGATAGGGGACGGAAGGTCGTTTGTTTTTGTTCTGAGCTCCATAAGAAC
  TGTGCTGACTTGGAAGGTGCCCTGTGCTATGTCGTGCCTGCAGGGACACGTCGGATCCCG
  TGGGCCTCACATGCCAGGTCACCAGGTCACCGTCTCCTTCCACCCCTGCAGTGTGCTGTT
  GTGCACGTCAGGGACGCTGTTCTCTATGCCCACTGCCCTCCTCCCTCTCCTGGCCCAGCA
  GTATTGCTCACGGGGGCTCCAGCCGCCGGCGTGGCCCTCATGAGCTACGCCTGGGTCTTC
  TGCAGACTCATGCAGCCCTATGGCCGCTCAGACCAAGGCGCAGAGCAACTATCAGGGCAG
  CTCTGCCTCCTCCTCCCATGAGGTGGGGAGAGGCAACAGGGCAGCCCCCAGAGGAGTGTC
  CTGGCCGCTGTCCTCCCGGGGCCCATGATGGCCATAGATTTGCCTTGTGGTGTTGGATCA
  GGTACTGTGTCTGCTCATAAGTACTTGTGTCATCCAGAATGTTTTGTTTTTTAAGAAAAT
  TGAATTACTTGTTTCCTGAAATATTCTGAGGTTAATATGTTAGTTTTCATAGAACATTGA
  GAGGCCCCTGCCACTTTCAATAAAGACCTGACTTGGAGACAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_006374
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006374.3, NP_006365.2
• RefSeq Size: 2198 bp
• RefSeq ORF: 1281 bp
• Locus ID: 10494
• UniProt ID: O00506
• Cytogenetics: 2q37.3
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: This gene encodes a member of the germinal centre kinase III (GCK III) subfamily of the sterile 20 superfamily of kinases. The encoded enzyme plays a role in serine-threonine liver kinase B1 (LKB1) signaling pathway to regulate neuronal polarization and morphology of the Golgi apparatus. The protein is translocated from the Golgi apparatus to the nucleus in response to chemical anoxia and plays a role in regulation of cell death. A pseudogene associated with this gene is located on chromosome 18. Multiple alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Dec 2012]
  Transcript Variant: This variant (4) differs in the 5' UTR compared to variant 1. Variants 1, 4 and 5 encode the same isoform (1).