TSPAN1 (NM_005727) Human Untagged Clone

CAT#: SC321265

TSPAN1 (untagged)-Human tetraspanin 1 (TSPAN1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TSPAN1
• Synonyms: NET1; TM4C; TM4SF
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_005727.2 GTGAGAGCCAGGCGTCCCTCTGCCTGCCCACTCAGTGGCAACACCCGGGAGCTGTTTTGT
  CCTTTGTGGAGCCTCAGCAGTTCCCTCTTTCAGAACTCACTGCCAAGAGCCCTGAACAGG
  AGCCACCATGCAGTGCTTCAGCTTCATTAAGACCATGATGATCCTCTTCAATTTGCTCAT
  CTTTCTGTGTGGTGCAGCCCTGTTGGCAGTGGGCATCTGGGTGTCAATCGATGGGGCATC
  CTTTCTGAAGATCTTCGGGCCACTGTCGTCCAGTGCCATGCAGTTTGTCAACGTGGGCTA
  CTTCCTCATCGCAGCCGGCGTTGTGGTCTTTGCTCTTGGTTTCCTGGGCTGCTATGGTGC
  TAAGACTGAGAGCAAGTGTGCCCTCGTGACGTTCTTCTTCATCCTCCTCCTCATCTTCAT
  TGCTGAGGTTGCAGCTGCTGTGGTCGCCTTGGTGTACACCACAATGGCTGAGCACTTCCT
  GACGTTGCTGGTAGTGCCTGCCATCAAGAAAGATTATGGTTCCCAGGAAGACTTCACTCA
  AGTGTGGAACACCACCATGAAAGGGCTCAAGTGCTGTGGCTTCACCAACTATACGGATTT
  TGAGGACTCACCCTACTTCAAAGAGAACAGTGCCTTTCCCCCATTCTGTTGCAATGACAA
  CGTCACCAACACAGCCAATGAAACCTGCACCAAGCAAAAGGCTCACGACCAAAAAGTAGA
  GGGTTGCTTCAATCAGCTTTTGTATGACATCCGAACTAATGCAGTCACCGTGGGTGGTGT
  GGCAGCTGGAATTGGGGGCCTCGAGCTGGCTGCCATGATTGTGTCCATGTATCTGTACTG
  CAATCTACAATAAGTCCACTTCTGCCTCTGCCACTACTGCTGCCACATGGGAACTGTGAA
  GAGGCACCCTGGCAAGCAGCAGTGATTGGGGGAGGGGACAGGATCTAACAATGTCACTTG
  GGCCAGAATGGACCTGCCCTTTCTGCTCCAGACTTGGGGCTAGATAGGGACCACTCCTTT
  TAGGCGATGCCTGACTTTCCTTCCATTGGTGGGTGGATGGGTGGGGGGCATTCCAGAGCC
  TCTAAGGTAGCCAGTTCTGTTGCCCATTCCCCCAGTCTATTAAACCCTTGATATGCCCCC
  TAGGCCTAGTGGTGATCCCAGTGCTCTACTGGGGGATGAGAGAAAGGCATTTTATAGCCT
  GGGCATAAGTGAAATCAGCAGAGCCTCTGGGTGGATGTGTAGAAGGCACTTCAAAATGCA
  TAAACCTGTTACAATGTTAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_005727
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005727.2, NP_005718.2
• RefSeq Size: 1297 bp
• RefSeq ORF: 726 bp
• Locus ID: 10103
• UniProt ID: O60635
• Cytogenetics: 1p34.1
• Domains: transmembrane4
• Protein Families: Transmembrane
• Gene Summary: The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. [provided by RefSeq, Jul 2008]