TIMP3 (NM_000362) Human Untagged Clone

CAT#: SC321254

TIMP3 (untagged)-Human TIMP metallopeptidase inhibitor 3 (TIMP3)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TIMP3
• Synonyms: HSMRK222; K222; K222TA2; SFD
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000362.4 GCTCCTCCAGCTCCTGCTCCTTCGCCGGGAGGCCGCCCGCCGAGTCCTGCGCCAGCGCCG
  AGGCAGCCTCGCTGCGCCCCATCCCGTCCCGCCGGGCACTCGGAGGGCAGCGCGCCGGAG
  GCCAAGGTTGCCCCGCACGGCCCGGCGGGCGAGCGAGCTCGGGCTGCAGCAGCCCCGCCG
  GCGGCGCGCACGGCAACTTTGGAGAGGCGAGCAGCAGCCCCGGCAGCGGCGGCAGCAGCG
  GCAATGACCCCTTGGCTCGGGCTCATCGTGCTCCTGGGCAGCTGGAGCCTGGGGGACTGG
  GGCGCCGAGGCGTGCACATGCTCGCCCAGCCACCCCCAGGACGCCTTCTGCAACTCCGAC
  ATCGTGATCCGGGCCAAGGTGGTGGGGAAGAAGCTGGTAAAGGAGGGGCCCTTCGGCACG
  CTGGTCTACACCATCAAGCAGATGAAGATGTACCGAGGCTTCACCAAGATGCCCCATGTG
  CAGTACATCCATACGGAAGCTTCCGAGAGTCTCTGTGGCCTTAAGCTGGAGGTCAACAAG
  TACCAGTACCTGCTGACAGGTCGCGTCTATGATGGCAAGATGTACACGGGGCTGTGCAAC
  TTCGTGGAGAGGTGGGACCAGCTCACCCTCTCCCAGCGCAAGGGGCTGAACTATCGGTAT
  CACCTGGGTTGTAACTGCAAGATCAAGTCCTGCTACTACCTGCCTTGCTTTGTGACTTCC
  AAGAACGAGTGTCTCTGGACCGACATGCTCTCCAATTTCGGTTACCCTGGCTACCAGTCC
  AAACACTACGCCTGCATCCGGCAGAAGGGCGGCTACTGCAGCTGGTACCGAGGATGGGCC
  CCCCCGGATAAAAGCATCATCAATGCCACAGACCCCTGAGCGCCAGACCCTGCCCCACCT
  CACTTCCCTCCCTTCCCGCTGAGCTTCCCTTGGACACTAACTCTTCCCAGATGATGACAA
  TGAAATTAGTGCCTGTTTTCTTGCAAATTTAGCACTTGGAACATTTAAAGAAAGGTCTAT
  GCTGTCATATGGGGTTTATTGGGAACTATCCTCCTGGCCCCACCCTGCCCCTTCTTTTTG
  GTTTTGACATCATTCATTTCCACCTGGGAATTTCTGGTGCCATGCCAGAAAGAATGAGGA
  ACCTGTATTCCTCTTCTTCGTGATAATATAATCTCTATTTTTTTAGGAAAACAAAAATGA
  AAAACTACTCCATTTGAGGATTGTAATTCCCACCCCTCTTGCTTCTTCCCCACCTCACCA
  TCTCCCAGACCCTCTTCCCTTTGCCCTTCTCCTCCAATACATAAAGGACACAGACAAGGA
  ACTTGCTGAAAGGCCAACCATTTCAGGATCAGTCAAAGGCAGCAAGCAGATAGACTCAAG
  GTGTGTGAAAGATGTTATACACCAGGAGCTGCCACTGCATGTCCCAACCAGACTGTGTCT
  GTCTGTGTCTGCATGTAAGAGTGAGGGAGGGAAGGAAGGAACTACAAGAGAGTCGGAGAT
  GATGCAGCACACACACAATTCCCCAGCCCAGTGATGCTTGTGTTGACCAGATGTTCCTGA
  GTCTGGAGCAAGCACCCAGGCCAGAATAACAGAGCTTTCTTAGTTGGTGAAGACTTAAAC
  ATCTGCCTGAGGTCAGGAGGCAATTTGCCTGCCTTGTACAAAAGCTCAGGTGAAAGACTG
  AGATGAATGTCTTTCCTCTCCCTGCCTCCCACCAGACTTCCTCCTGGAAAACGCTTTGGT
  AGATTTGGCCAGGAGCTTTCTTTTATGTAAATTGGATAAATACACACACCATACACTATC
  CACAGATATAGCCAAGTAGATTTGGGTAGAGGATACTATTTCCAGAATAGTGTTTAGCTC
  ACCTAGGGGGATATGTTTGTATACACATTTGCATATACCCACATGGGGACATAAGCTAAT
  TTTTTTACAGGACACAGAATTCTGTTCAATGCTGTTAAATATGCCAATAGTTTAATCTCT
  TCTATTTTGTTGTCGTTGCTTGTTTGAAGAAAATCATGACATTCCAAGTTGACATTTTTT
  TTTCATTTTAATTAAAATTTGAAATTCTGAACACCGTCAGCACCCTCTCTTCCCTATCAT
  GGGTCATCTGACCCCTGTCCGTCTCCTTGTCCCTGCTTCATGTTTGGGGGCCTTTCTTTA
  ACTGCCTTCCTGGCTTAGCTCAGATGGCAGATGAGAGTGTAGTCAAGGGCCTGGGCACAG
  GAGGGAGAGCTGCAGAGTGTCCTGCCTGCCTTGGCTGGAGGGACACCTCTCCTGGGTGTG
  GAGACAGCTTGGTTCCCTTTCCCTAGCTCCCTGGTGGGTGAATGCCACCTCCTGAGATCC
  TCACCTCTTGGAATTAAAATTGTTGGTCACTGGGGAAAGCCTGAGTTTGCAACCAGTTGT
  AGGGTTTCTGTTGTGTTTTTTTTTTTTTTTTTGAAATAAAACTATAATATAAATTCTCCT
  ATTAAATAAAATTATTTTAAGTTTTAGTGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_000362
• Insert Size: 2500 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000362.4, NP_000353.1
• RefSeq Size: 5496 bp
• RefSeq ORF: 636 bp
• Locus ID: 7078
• UniProt ID: P35625
• Cytogenetics: 22q12.3
• Domains: NTR
• Protein Families: Druggable Genome, Secreted Protein
• Gene Summary: This gene belongs to the TIMP gene family. The proteins encoded by this gene family are inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix (ECM). Expression of this gene is induced in response to mitogenic stimulation and this netrin domain-containing protein is localized to the ECM. Mutations in this gene have been associated with the autosomal dominant disorder Sorsby's fundus dystrophy. [provided by RefSeq, Jul 2008]