HEXB (NM_000521) Human Untagged Clone

CAT#: SC321203

HEXB (untagged)-Human hexosaminidase B (beta polypeptide) (HEXB)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HEXB
• Synonyms: ENC-1AS; HEL-248; HEL-S-111
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000521.2 GGCGGAAAGCAGCCGAGCGGCCATGGAGCTGTGCGGGCTGGGGCTGCCCCGGCCGCCCAT
  GCTGCTGGCGCTGCTGTTGGCGACACTGCTGGCGGCGATGTTGGCGCTGCTGACTCAGGT
  GGCGCTGGTGGTGCAGGTGGCGGAGGCGGCTCGGGCCCCGAGCGTCTCGGCCAAGCCGGG
  GCCGGCGCTGTGGCCCCTGCCGCTCTCGGTGAAGATGACCCCGAACCTGCTGCATCTCGC
  CCCGGAGAACTTCTACATCAGCCACAGCCCCAATTCCACGGCGGGCCCCTCCTGCACCCT
  GCTGGAGGAAGCGTTTCGACGATATCATGGCTATATTTTTGGTTTCTACAAGTGGCATCA
  TGAACCTGCTGAATTCCAGGCTAAAACCCAGGTTCAGCAACTTCTTGTCTCAATCACCCT
  TCAGTCAGAGTGTGATGCTTTCCCCAACATATCTTCAGATGAGTCTTATACTTTACTTGT
  GAAAGAACCAGTGGCTGTCCTTAAGGCCAACAGAGTTTGGGGAGCATTACGAGGTTTAGA
  GACCTTTAGCCAGTTAGTTTATCAAGATTCTTATGGAACTTTCACCATCAATGAATCCAC
  CATTATTGATTCTCCAAGGTTTTCTCACAGAGGAATTTTGATTGATACATCCAGACATTA
  TCTGCCAGTTAAGATTATTCTTAAAACTCTGGATGCCATGGCTTTTAATAAGTTTAATGT
  TCTTCACTGGCACATAGTTGATGACCAGTCTTTCCCATATCAGAGCATCACTTTTCCTGA
  GTTAAGCAATAAAGGAAGCTATTCTTTGTCTCATGTTTATACACCAAATGATGTCCGTAT
  GGTGATTGAATATGCCAGATTACGAGGAATTCGAGTCCTGCCAGAATTTGATACCCCTGG
  GCATACACTATCTTGGGGAAAAGGTCAGAAAGACCTCCTGACTCCATGTTACAGTAGACA
  AAACAAGTTGGACTCTTTTGGACCTATAAACCCTACTCTGAATACAACATACAGCTTCCT
  TACTACATTTTTCAAAGAAATTAGTGAGGTGTTTCCAGATCAATTCATTCATTTGGGAGG
  AGATGAAGTGGAATTTAAATGTTGGGAATCAAATCCAAAAATTCAAGATTTCATGAGGCA
  AAAAGGCTTTGGCACAGATTTTAAGAAACTAGAATCTTTCTACATTCAAAAGGTTTTGGA
  TATTATTGCAACCATAAACAAGGGATCCATTGTCTGGCAGGAGGTTTTTGATGATAAAGC
  AAAGCTTGCGCCGGGCACAATAGTTGAAGTATGGAAAGACAGCGCATATCCTGAGGAACT
  CAGTAGAGTCACAGCATCTGGCTTCCCTGTAATCCTTTCTGCTCCTTGGTACTTAGATTT
  GATTAGCTATGGACAAGATTGGAGGAAATACTATAAAGTGGAACCTCTTGATTTTGGCGG
  TACTCAGAAACAGAAACAACTTTTCATTGGTGGAGAAGCTTGTCTATGGGGAGAATATGT
  GGATGCAACTAACCTCACTCCAAGATTATGGCCTCGGGCAAGTGCTGTTGGTGAGAGACT
  CTGGAGTTCCAAAGATGTCAGAGATATGGATGACGCCTATGACAGACTGACAAGGCACCG
  CTGCAGGATGGTCGAACGTGGAATAGCTGCACAACCTCTTTATGCTGGATATTGTAACCA
  TGAGAACATGTAAAAAATGGAGGGGAAAAAGGCCACAGCAATCTGTACTACAATCAACTT
  TATTTTGAAATCATGTAAAATAAGATATTAGACTGTTTTTTGAATAAAATATTTTTATTG
  ATTGAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_000521
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000521.2, NP_000512.1
• RefSeq Size: 1857 bp
• RefSeq ORF: 1671 bp
• Locus ID: 3074
• UniProt ID: P07686
• Cytogenetics: 5q13.3
• Domains: Glyco_hydro_20
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Amino sugar and nucleotide sugar metabolism, Glycosaminoglycan degradation, Glycosphingolipid biosynthesis - ganglio series, Glycosphingolipid biosynthesis - globo series, Lysosome, Metabolic pathways, Other glycan degradation
• Gene Summary: Hexosaminidase B is the beta subunit of the lysosomal enzyme beta-hexosaminidase that, together with the cofactor GM2 activator protein, catalyzes the degradation of the ganglioside GM2, and other molecules containing terminal N-acetyl hexosamines. Beta-hexosaminidase is composed of two subunits, alpha and beta, which are encoded by separate genes. Both beta-hexosaminidase alpha and beta subunits are members of family 20 of glycosyl hydrolases. Mutations in the alpha or beta subunit genes lead to an accumulation of GM2 ganglioside in neurons and neurodegenerative disorders termed the GM2 gangliosidoses. Beta subunit gene mutations lead to Sandhoff disease (GM2-gangliosidosis type II). Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2014]
  Transcript Variant: This variant (1) encodes the longer isoform (1).