FUCA2 (NM_032020) Human Untagged Clone

CAT#: SC320995

FUCA2 (untagged)-Human fucosidase, alpha-L- 2, plasma (FUCA2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: FUCA2
• Synonyms: dJ20N2.5
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_032020.3 GGCATACTCAGCGGCAGAGACGGAAGAACAGCGCTCCCGAGGCCGCGGGAGCCTGCAGAG
  AGGACAGCCGGCCTGCGCCGGGACATGCGGCCCCAGGAGCTCCCCAGGCTCGCGTTCCCG
  TTGCTGCTGTTGCTGTTGCTGCTGCTGCCGCCGCCGCCGTGCCCTGCCCACAGCGCCACG
  CGCTTCGACCCCACCTGGGAGTCCCTGGACGCCCGCCAGCTGCCCGCGTGGTTTGACCAG
  GCCAAGTTCGGCATCTTCATCCACTGGGGAGTGTTTTCCGTGCCCAGCTTCGGTAGCGAG
  TGGTTCTGGTGGTATTGGCAAAAGGAAAAGATACCGAAGTATGTGGAATTTATGAAAGAT
  AATTACCCTCCTAGTTTCAAATATGAAGATTTTGGACCACTATTTACAGCAAAATTTTTT
  AATGCCAACCAGTGGGCAGATATTTTTCAGGCCTCTGGTGCCAAATACATTGTCTTAACT
  TCCAAACATCATGAAGGCTTTACCTTGTGGGGGTCAGAATATTCGTGGAACTGGAATGCC
  ATAGATGAGGGGCCCAAGAGGGACATTGTCAAGGAACTTGAGGTAGCCATTAGGAACAGA
  ACTGACCTGCGTTTTGGACTGTACTATTCCCTTTTTGAATGGTTTCATCCGCTCTTCCTT
  GAGGATGAATCCAGTTCATTCCATAAGCGGCAATTTCCAGTTTCTAAGACATTGCCAGAG
  CTCTATGAGTTAGTGAACAACTATCAGCCTGAGGTTCTGTGGTCGGATGGTGACGGAGGA
  GCACCGGATCAATACTGGAACAGCACAGGCTTCTTGGCCTGGTTATATAATGAAAGCCCA
  GTTCGGGGCACAGTAGTCACCAATGATCGTTGGGGAGCTGGTAGCATCTGTAAGCATGGT
  GGCTTCTATACCTGCAGTGATCGTTATAACCCAGGACATCTTTTGCCACATAAATGGGAA
  AACTGCATGACAATAGACAAACTGTCCTGGGGCTATAGGAGGGAAGCTGGAATCTCTGAC
  TATCTTACAATTGAAGAATTGGTGAAGCAACTTGTAGAGACAGTTTCATGTGGAGGAAAT
  CTTTTGATGAATATTGGGCCCACACTAGATGGCACCATTTCTGTAGTTTTTGAGGAGCGA
  CTGAGGCAAGTGGGGTCCTGGCTAAAAGTCAATGGAGAAGCTATTTATGAAACCTATACC
  TGGCGATCCCAGAATGACACTGTCACCCCAGATGTGTGGTACACATCCAAGCCTAAAGAA
  AAATTAGTCTATGCCATTTTTCTTAAATGGCCCACATCAGGACAGCTGTTCCTTGGCCAT
  CCCAAAGCTATTCTGGGGGCAACAGAGGTGAAACTACTGGGCCATGGACAGCCACTTAAC
  TGGATTTCTTTGGAGCAAAATGGCATTATGGTAGAACTGCCACAGCTAACCATTCATCAG
  ATGCCGTGTAAATGGGGCTGGGCTCTAGCCCTAACTAATGTGATCTAAAGTGCAGCAGAG
  TGGCTGATGCTGCAAGTTATGTCTAAGGCTAGGAACTATCAGGTGTCTATAATTGTAGCA
  CATGGAGAAAGCAAATGTAAAACTGGATAAGAAAATTATTTTGGCAGTTCAGCCCTTTCC
  CTTTTTCCCACTAAATTTTTTCTTAAATTACCCATGTAACCATTTTAACTCTCCAGTGCA
  CTTTGCCATTAAAGTCTCTTCACATTGAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_032020
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032020.3, NP_114409.2
• RefSeq Size: 2098 bp
• RefSeq ORF: 1404 bp
• Locus ID: 2519
• UniProt ID: Q9BTY2
• Cytogenetics: 6q24.2
• Domains: Alpha_L_fucos
• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Other glycan degradation
• Gene Summary: This gene encodes a plasma alpha-L-fucosidase, which represents 10-20% of the total cellular fucosidase activity. The protein is a member of the glycosyl hydrolase 29 family, and catalyzes the hydrolysis of the alpha-1,6-linked fucose joined to the reducing-end N-acetylglucosamine of the carbohydrate moieties of glycoproteins. This enzyme is essential for Helicobacter pylori adhesion to human gastric cancer cells. [provided by RefSeq, Aug 2010]