Glutamine Synthetase (GLUL) (NM_001033056) Human Untagged Clone

CAT#: SC320897

GLUL (untagged)-Human glutamate-ammonia ligase (GLUL), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Glutamine Synthetase
• Synonyms: GLNS; GS; PIG43; PIG59
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001033056.1 GAAGAGCGGAGCGTGTGAGCAGTACTGCGGCCTCCTCTCCTCTCCTAACCTCGCTCTCGC
  GGCCTACCTTTACCCGCCCGCCTGCTCGGCGACCAGAACACCTTCCACCATGACCACCTC
  AGCAAGTTCCCACTTAAATAAAGGCATCAAGCAGGTGTACATGTCCCTGCCTCAGGGTGA
  GAAAGTCCAGGCCATGTATATCTGGATCGATGGTACTGGAGAAGGACTGCGCTGCAAGAC
  CCGGACCCTGGACAGTGAGCCCAAGTGTGTGGAAGAGTTGCCTGAGTGGAATTTCGATGG
  CTCCAGTACTTTACAGTCTGAGGGTTCCAACAGTGACATGTATCTCGTGCCTGCTGCCAT
  GTTTCGGGACCCCTTCCGTAAGGACCCTAACAAGCTGGTGTTATGTGAAGTTTTCAAGTA
  CAATCGAAGGCCTGCAGAGACCAATTTGAGGCACACCTGTAAACGGATAATGGACATGGT
  GAGCAACCAGCACCCCTGGTTTGGCATGGAGCAGGAGTATACCCTCATGGGGACAGATGG
  GCACCCCTTTGGTTGGCCTTCCAACGGCTTCCCAGGGCCCCAGGGTCCATATTACTGTGG
  TGTGGGAGCAGACAGAGCCTATGGCAGGGACATCGTGGAGGCCCATTACCGGGCCTGCTT
  GTATGCTGGAGTCAAGATTGCGGGGACTAATGCCGAGGTCATGCCTGCCCAGTGGGAATT
  TCAGATTGGACCTTGTGAAGGAATCAGCATGGGAGATCATCTCTGGGTGGCCCGTTTCAT
  CTTGCATCGTGTGTGTGAAGACTTTGGAGTGATAGCAACCTTTGATCCTAAGCCCATTCC
  TGGGAACTGGAATGGTGCAGGCTGCCATACCAACTTCAGCACCAAGGCCATGCGGGAGGA
  GAATGGTCTGAAGTACATCGAGGAGGCCATTGAGAAACTAAGCAAGCGGCACCAGTACCA
  CATCCGTGCCTATGATCCCAAGGGAGGCCTGGACAATGCCCGACGTCTAACTGGATTCCA
  TGAAACCTCCAACATCAACGACTTTTCTGCTGGTGTAGCCAATCGTAGCGCCAGCATACG
  CATTCCCCGGACTGTTGGCCAGGAGAAGAAGGGTTACTTTGAAGATCGTCGCCCCTCTGC
  CAACTGCGACCCCTTTTCGGTGACAGAAGCCCTCATCCGCACGTGTCTTCTCAATGAAAC
  CGGCGATGAGCCCTTCCAGTACAAAAATTAAGTGGACTAGACCTCCAGCTGTTGAGCCCC
  TCCTAGTTCTTCATCCCACTCCAACTCTTCCCCCTCTCCCAGTTGTCCCGATTGTAACTC
  AAAGGGTGGAATATCAAGGTCGTTTTTTTCATTCCATGTGCCCAGTTAATCTTGCTTTCT
  TTGTTTGGCTGGGATAGAGGGGTCAAGTTATTAATTTCTTCACACCTACCCTCCTTTTTT
  TCCCTATCACTGAAGCTTTTTAGTGCATTAGTGGGGAGGAGGGTGGGGAGACATAACCAC
  TGCTTCCATTTAATGGGGTGCACCTGTCCAATAGGCGTAGCTATCCGGACAGAGCACGTT
  TGCAGAAGGGGGACTCTTCTTCCAGGTAGCTGAAAGGGGAAGACCTGACGTACTCTGGTT
  AGGTTAGGACTTGCCCTCGTGGTGGAAACTTTTCTTAAAAAGTTATAACCAACTTTTCTA
  TTAAAAGTGGGAATTAGGAGAGAAGGTAGGGGTTGGGAATCAGAGAGAATGGCTTTGGTC
  TCTTGCTTGTGGGACTAGCCTGGCTTGGGACTAAATGCCCTGCTCTGAACACGAAGCTTA
  GTATAAACTGATGGATATCCCTACCTTGAAAGAAGAAAAGGTTCTTACTGCTTGGTCCTT
  GATTTATCACACAAAGCAGAATAGTATTTTTATATTTAAATGTAAAGACAAAAAACTATA
  TGTATGGTTTTGTGGATTATGTGTGTTTTGCTAAAGGAAAAAACCATCCAGGTCACGGGG
  CACCAAATTTGAGACAAATAGTCGGATTAGAAATAAAGCATCTCATTTTGAGTAGAGAGC
  AAGGGAAGTGGTTCTTAGATGGTGATCTGGGATTAGGCCCTCAAGACCCTTTTGGGTTTC
  TGCCCTGCCCACCCTCTGGAGAAGGTGGGCACTGGATTAGTTAACAGACGACACGTTACT
  AGCAGTCACTTGATCTCCGTGGCTTTGGTTTAAAAGACACACTTGTCCACATAGGTTTAG
  AGATAAGAGTTGGCTGGTCAACTTGAGCATGTTACTGACAGAGGGGGTATTGGGGTTATT
  TTCTGGTAGGAATAGCATGTCACTAAAGCAGGCCTTTTGATATTAAATTTTTTAAAAAGC
  AAAATTATAGAAGTTTAGATTTTAATCAAATTTGTAGGGTTTCTAGGTAATTTTTACAGA
  ATTGCTTGTTTGCTTCAACTGTCTCCTACCTCTGCTCTTGGAGGAGATGGGGACAGGGCT
  GGAGTCAAAACACTTGTAATTTTGTATCTTGATGTCTTTGTTAAGACTGCTGAAGAATTA
  TTTTTTTCTTTTATAATAAGGAATAAACCCCACCTTTATTCCTTCATTTCATCTACCATT
  TTCTGGTTCTTGTGTTGGCTGTGGCAGGCCAGCTGTGGTTTTCTTTTGCCATGACAACTT
  CTAATTGCCATGTACAGTATGTTCAAAGTCAAATAACTCCTCATTGTAAACAAACTGTGT
  AACTGCCCAAAGCAGCACTTATAAATCAGCCTAACATAAGAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_001033056
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001033056.1, NP_001028228.1
• RefSeq Size: 3205 bp
• RefSeq ORF: 1122 bp
• Locus ID: 2752
• UniProt ID: P15104
• Cytogenetics: 1q25.3
• Protein Pathways: Alanine, aspartate and glutamate metabolism, Arginine and proline metabolism, Metabolic pathways, Nitrogen metabolism
• Gene Summary: The protein encoded by this gene belongs to the glutamine synthetase family. It catalyzes the synthesis of glutamine from glutamate and ammonia in an ATP-dependent reaction. This protein plays a role in ammonia and glutamate detoxification, acid-base homeostasis, cell signaling, and cell proliferation. Glutamine is an abundant amino acid, and is important to the biosynthesis of several amino acids, pyrimidines, and purines. Mutations in this gene are associated with congenital glutamine deficiency, and overexpression of this gene was observed in some primary liver cancer samples. There are six pseudogenes of this gene found on chromosomes 2, 5, 9, 11, and 12. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]
  Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. Both variants 1, 2 and 3 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.