PPP2R1A (NM_014225) Human Untagged Clone

CAT#: SC320858

PPP2R1A (untagged)-Human protein phosphatase 2, regulatory subunit A, alpha (PPP2R1A), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PPP2R1A
• Synonyms: MRD36; PP2A-Aalpha; PP2AA; PP2AAALPHA; PR65A
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_014225.3 GGCACGAGGCAGCGCTGGCCGCAGTCTGACAGGAAAGGGACGGAGCCAAGATGGCGGCGG
  CCGACGGCGACGACTCGCTGTACCCCATCGCGGTGCTCATAGACGAACTCCGCAATGAGG
  ACGTTCAGCTTCGCCTCAACAGCATCAAGAAGCTGTCCACCATCGCCTTGGCCCTTGGGG
  TTGAAAGGACCCGAAGTGAGCTTCTGCCTTTCCTTACAGATACCATCTATGATGAAGATG
  AGGTCCTCCTGGCCCTGGCAGAACAGCTGGGAACCTTCACTACCCTGGTGGGAGGCCCAG
  AGTACGTGCACTGCCTGCTGCCACCGCTGGAGTCGCTGGCCACAGTGGAGGAGACAGTGG
  TGCGGGACAAGGCAGTGGAGTCCTTACGGGCCATCTCACACGAGCACTCGCCCTCTGACC
  TGGAGGCGCACTTTGTGCCGCTAGTGAAGCGGCTGGCGGGCGGCGACTGGTTCACCTCCC
  GCACCTCGGCCTGCGGCCTCTTCTCCGTCTGCTACCCCCGAGTGTCCAGTGCTGTGAAGG
  CGGAACTTCGACAGTACTTCCGGAACCTGTGCTCAGATGACACCCCCATGGTGCGGCGGG
  CCGCAGCCTCCAAGCTGGGGGAGTTTGCCAAGGTGCTGGAGCTGGACAACGTCAAGAGTG
  AGATCATCCCCATGTTCTCCAACCTGGCCTCTGACGAGCAGGACTCGGTGCGGCTGCTGG
  CGGTGGAGGCGTGCGTGAACATCGCCCAGCTTCTGCCCCAGGAGGATCTGGAGGCCCTGG
  TGATGCCCACTCTGCGCCAGGCCGCTGAAGACAAGTCCTGGCGCGTCCGCTACATGGTGG
  CTGACAAGTTCACAGAGCTCCAGAAAGCAGTGGGGCCTGAGATCACCAAGACAGACCTGG
  TCCCTGCCTTCCAGAACCTGATGAAAGACTGTGAGGCCGAGGTGAGGGCCGCAGCCTCCC
  ACAAGGTCAAAGAGTTCTGTGAAAACCTCTCAGCTGACTGTCGGGAGAATGTGATCATGT
  CCCAGATCTTGCCCTGCATCAAGGAGCTGGTGTCCGATGCCAACCAACATGTCAAGTCTG
  CCCTGGCCTCAGTCATCATGGGTCTCTCTCCCATCTTGGGCAAAGACAACACCATCGAGC
  ACCTCTTGCCCCTCTTCCTGGCTCAGCTGAAGGATGAGTGCCCTGAGGTACGGCTGAACA
  TCATCTCTAACCTGGACTGTGTGAACGAGGTGATTGGCATCCGGCAGCTGTCCCAGTCCC
  TGCTCCCTGCCATTGTGGAGCTGGCTGAGGACGCCAAGTGGCGGGTGCGGCTGGCCATCA
  TTGAGTACATGCCCCTCCTGGCTGGACAGCTGGGAGTGGAGTTCTTTGATGAGAAACTTA
  ACTCCTTGTGCATGGCCTGGCTTGTGGATCATGTATATGCCATCCGCGAGGCAGCCACCA
  GCAACCTGAAGAAGCTAGTGGAAAAGTTTGGGAAGGAGTGGGCCCATGCCACAATCATCC
  CCAAGGTCTTGGCCATGTCCGGAGACCCCAACTACCTGCACCGCATGACTACGCTCTTCT
  GCATCAATGTGCTGTCTGAGGTCTGTGGGCAGGACATCACCACCAAGCACATGCTACCCA
  CGGTTCTGCGCATGGCTGGGGACCCGGTTGCCAATGTCCGCTTCAATGTGGCCAAGTCTC
  TGCAGAAGATAGGGCCCATCCTGGACAACAGCACCTTGCAGAGTGAAGTCAAGCCCATCC
  TAGAGAAGCTGACCCAGGACCAGGATGTGGACGTCAAATACTTTGCCCAGGAGGCTCTGA
  CTGTTCTGTCTCTCGCCTGATGCTGGAAGAGGAGCAAACACTGGCCTCTGGTGTCCACCC
  TCCAACCCCCACAAGTCCCTCTTTGGGGAGACACTGGGGGGCCTTTGGCTGTCACTCCCT
  GTGCATGGTCTGACCCCAGGCCCCTTCCCCCAGCACGGTTCCTCCTCTCCCCAGCCTGGG
  AAGATGTCTCACTGTCCACCTCCCAACGGGCTAGGGGAGCACGGGGTTGGACAGGACAGT
  GACCTTGGGAGGAAGGGGCTACTCCGCCCACGTCAGGGAGAGATGTGAGCATCCCGGGTC
  ACTGGATCCTGCTGCTGTAATGGGAACCCCTCCCCCATTTACTTCTCCACCTCCCGTCCT
  CCCCATCATTGGTTTTTTTTTGTGTGTCAACTGTGCCGTTTTTATTTTATTCCTTTTATT
  TTCCCCCTTTTCACAGAGAAATAAAGGTCTAGAAGTAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_014225
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014225.3, NP_055040.2
• RefSeq Size: 2357 bp
• RefSeq ORF: 1770 bp
• Locus ID: 5518
• UniProt ID: P30153
• Cytogenetics: 19q13.41
• Domains: HEAT, HEAT_PBS
• Protein Families: Druggable Genome, Phosphatase, Transcription Factors
• Protein Pathways: Long-term depression, Oocyte meiosis, TGF-beta signaling pathway, Tight junction, Wnt signaling pathway
• Gene Summary: This gene encodes a constant regulatory subunit of protein phosphatase 2. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The constant regulatory subunit A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. This gene encodes an alpha isoform of the constant regulatory subunit A. Alternatively spliced transcript variants have been described. [provided by RefSeq, Apr 2010]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the functional protein.