Ezrin (EZR) (NM_003379) Human Untagged Clone

CAT#: SC320852

EZR (untagged)-Human ezrin (EZR), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Ezrin
• Synonyms: CVIL; CVL; HEL-S-105; VIL2
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_003379.3 CGCGGCAGGAGGACGAGGCAGGGCGGGCGGGCGCTCTAAGGGTTCTGCTCTGACTCCAGG
  TTGGGACAGCGTCTTCGCTGCTGCTGGATAGTCGTGTTTTCGGGGATCGAGGATACTCAC
  CAGAAACCGAAAATGCCGAAACCAATCAATGTCCGAGTTACCACCATGGATGCAGAGCTG
  GAGTTTGCAATCCAGCCAAATACAACTGGAAAACAGCTTTTTGATCAGGTGGTAAAGACT
  ATCGGCCTCCGGGAAGTGTGGTACTTTGGCCTCCACTATGTGGATAATAAAGGATTTCCT
  ACCTGGCTGAAGCTGGATAAGAAGGTGTCTGCCCAGGAGGTCAGGAAGGAGAATCCCCTC
  CAGTTCAAGTTCCGGGCCAAGTTCTACCCTGAAGATGTGGCTGAGGAGCTCATCCAGGAC
  ATCACCCAGAAACTTTTCTTCCTCCAAGTGAAGGAAGGAATCCTTAGCGATGAGATCTAC
  TGCCCCCCTGAGACTGCCGTGCTCTTGGGGTCCTACGCTGTGCAGGCCAAGTTTGGGGAC
  TACAACAAAGAAGTGCACAAGTCTGGGTACCTCAGCTCTGAGCGGCTGATCCCTCAAAGA
  GTGATGGACCAGCACAAACTTACCAGGGACCAGTGGGAGGACCGGATCCAGGTGTGGCAT
  GCGGAACACCGTGGGATGCTCAAAGATAATGCTATGTTGGAATACCTGAAGATTGCTCAG
  GACCTGGAAATGTATGGAATCAACTATTTCGAGATAAAAAACAAGAAAGGAACAGACCTT
  TGGCTTGGAGTTGATGCCCTTGGACTGAATATTTATGAGAAAGATGATAAGTTAACCCCA
  AAGATTGGCTTTCCTTGGAGTGAAATCAGGAACATCTCTTTCAATGACAAAAAGTTTGTC
  ATTAAACCCATCGACAAGAAGGCACCTGACTTTGTGTTTTATGCCCCACGTCTGAGAATC
  AACAAGCGGATCCTGCAGCTCTGCATGGGCAACCATGAGTTGTATATGCGCCGCAGGAAG
  CCTGACACCATCGAGGTGCAGCAGATGAAGGCCCAGGCCCGGGAGGAGAAGCATCAGAAG
  CAGCTGGAGCGGCAACAGCTGGAAACAGAGAAGAAAAGGAGAGAAACCGTGGAGAGAGAG
  AAAGAGCAGATGATGCGCGAGAAGGAGGAGTTGATGCTGCGGCTGCAGGACTATGAGGAG
  AAGACAAAGAAGGCAGAGAGAGAGCTCTCGGAGCAGATTCAGAGGGCCCTGCAGCTGGAG
  GAGGAGAGGAAGCGGGCACAGGAGGAGGCCGAGCGCCTAGAGGCTGACCGTATGGCTGCA
  CTGCGGGCTAAGGAGGAGCTGGAGAGACAGGCGGTGGATCAGATAAAGAGCCAGGAGCAG
  CTGGCTGCGGAGCTTGCAGAATACACTGCCAAGATTGCCCTCCTGGAAGAGGCGCGGAGG
  CGCAAGGAGGATGAAGTTGAAGAGTGGCAGCACAGGGCCAAAGAAGCCCAGGATGACCTG
  GTGAAGACCAAGGAGGAGCTGCACCTGGTGATGACAGCACCCCCGCCCCCACCACCCCCC
  GTGTACGAGCCGGTGAGCTACCATGTCCAGGAGAGCTTGCAGGATGAGGGCGCAGAGCCC
  ACGGGCTACAGCGCGGAGCTGTCTAGTGAGGGCATCCGGGATGACCGCAATGAGGAGAAG
  CGCATCACTGAGGCAGAGAAGAACGAGCGTGTGCAGCGGCAGCTGCTGACGCTGAGCAGC
  GAGCTGTCCCAGGCCCGAGATGAGAATAAGAGGACCCACAATGACATCATCCACAACGAG
  AACATGAGGCAAGGCCGGGACAAGTACAAGACGCTGCGGCAGATCCGGCAGGGCAACACC
  AAGCAGCGCATCGACGAGTTCGAGGCCCTGTAACAGCCAGGCCAGGACCAAGGGCAGAGG
  GGTGCTCATAGCGGGCGCTGCCAGCCCCGCCACGCTTGTGTCTTTAGTGCTCCAAGTCTA
  GGAACTCCCTCAGATCCCAGTTCCTTTAGAAAGCAGTTACCCAACAGAAACATTCTGGGC
  TGGGAACCAGGGAGGCGCCCTGGTTTGTTTTCCCCAGTTGTAATAGTGCCAAGCAGGCCT
  GATTCTCGCGATTATTCTCGAATCACCTCCTGTGTTGTGCTGGGAGCAGGACTGATTGAA
  TTACGGAAAATGCCTGTAAAGTCTGAGTAAGAAACTTCATGCTGGCCTGTGTGATACAAG
  AGTCAGCATCATTAAAGGAAACGTGGCAGGACTTCCATCTGTGCCATACTTGTTCTGTAT
  TCGAAATGAGCTCAAATTGATTTTTTAATTTCTATGAAGGATCCATCTTTGTATATTTAC
  ATGCTTAGAGGGGTGAAAATTATTTTGGAAATTGAGTCTGAAGCACTCTCGCACACACAG
  TGATTCCCTCCTCCCGTCACTCCACGCAGCTGGCAGAGAGCACAGTGATCACCAGCGTGA
  GTGGTGGAGGAGGACACTTGGATTTTTTTTTTTGTTTTTTTTTTTTTTGCTTAACAGTTT
  TAGAATACATTGTACTTATACACCTTATTAATGATCAGCTATATACTATTTATATACAAG
  TGATAATACAGATTTGTAACATTAGTTTTAAAAAGGGAAAGTTTTGTTCTGTATATTTTG
  TTACCTTTTACAGAATAAAAGAATTACATATGAAAAACCCTCTAAACCATGGCACTTGAT
  GTGATGTGGCAGGAGGGCAGTGGTGGAGCTGGACCTGCCTGCTGCAGTCACGTGTAAACA
  GGATTATTATTAGTGTTTTATGCATGTAATGGACTATGCACACTTTTAATTTTGTCAGAT
  TCACACATGCCACTATGAGCTTTCAGACTCCAGCTGTGAAGAGACTCTGTTTGCTTGTGT
  TTGTTTGTTTGCAGTCTCTCTCTGCCATGGCCTTGGCAGGCTGCTGGAAGGCAGCTTGTG
  GAGGCCGTTGGTTCCGCCCACTCATTCCTTCTCGTGCACTGCTTTCTCCTTCACAGCTAA
  GATGCCATGTGCAGGTGGATTCCATGCCGCAGACATGAAATAAAAGCTTTGCAAAGGCAC
  GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_003379
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003379.3, NP_003370.2
• RefSeq Size: 3166 bp
• RefSeq ORF: 1761 bp
• Locus ID: 7430
• UniProt ID: P15311
• Cytogenetics: 6q25.3
• Domains: B41, ERM
• Protein Families: Druggable Genome
• Protein Pathways: Leukocyte transendothelial migration, Pathogenic Escherichia coli infection, Regulation of actin cytoskeleton
• Gene Summary: The cytoplasmic peripheral membrane protein encoded by this gene functions as a protein-tyrosine kinase substrate in microvilli. As a member of the ERM protein family, this protein serves as an intermediate between the plasma membrane and the actin cytoskeleton. This protein plays a key role in cell surface structure adhesion, migration and organization, and it has been implicated in various human cancers. A pseudogene located on chromosome 3 has been identified for this gene. Alternatively spliced variants have also been described for this gene. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) represents the longer transcript. Both variants 1 and 2 encode the same protein.