BAG3 (NM_004281) Human Untagged Clone

CAT#: SC320765

BAG3 (untagged)-Human BCL2-associated athanogene 3 (BAG3)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: BAG3
• Synonyms: BAG-3; BIS; CAIR-1; MFM6
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_004281.3 GGGCCGCGGCCAACTTCTCTGGACTGGACCAGAAGTTTCTAGCCGGCCAGTTGCTACCTC
  CCTTTATCTCCTCCTTCCCCTCTGGCAGCGAGGAGGCTATTTCCAGACACTTCCACCCCT
  CTCTGGCCACGTCACCCCCGCCTTTAATTCATAAAGGTGCCCGGCGCCGGCTTCCCGGAC
  ACGTCGGCGGCGGAGAGGGGCCCACGGCGGCGGCCCGGCCAGAGACTCGGCGCCCGGAGC
  CAGCGCCCCGCACCCGCGCCCCAGCGGGCAGACCCCAACCCAGCATGAGCGCCGCCACCC
  ACTCGCCCATGATGCAGGTGGCGTCCGGCAACGGTGACCGCGACCCTTTGCCCCCCGGAT
  GGGAGATCAAGATCGACCCGCAGACCGGCTGGCCCTTCTTCGTGGACCACAACAGCCGCA
  CCACTACGTGGAACGACCCGCGCGTGCCCTCTGAGGGCCCCAAGGAGACTCCATCCTCTG
  CCAATGGCCCTTCCCGGGAGGGCTCTAGGCTGCCGCCTGCTAGGGAAGGCCACCCTGTGT
  ACCCCCAGCTCCGACCAGGCTACATTCCCATTCCTGTGCTCCATGAAGGCGCTGAGAACC
  GGCAGGTGCACCCTTTCCATGTCTATCCCCAGCCTGGGATGCAGCGATTCCGAACTGAGG
  CGGCAGCAGCGGCTCCTCAGAGGTCCCAGTCACCTCTGCGGGGCATGCCAGAAACCACTC
  AGCCAGATAAACAGTGTGGACAGGTGGCAGCGGCGGCGGCAGCCCAGCCCCCAGCCTCCC
  ACGGACCTGAGCGGTCCCAGTCTCCAGCTGCCTCTGACTGCTCATCCTCATCCTCCTCGG
  CCAGCCTGCCTTCCTCCGGCAGGAGCAGCCTGGGCAGTCACCAGCTCCCGCGGGGGTACA
  TCTCCATTCCGGTGATACACGAGCAGAACGTTACCCGGCCAGCAGCCCAGCCCTCCTTCC
  ACCAAGCCCAGAAGACGCACTACCCAGCGCAGCAGGGGGAGTACCAGACCCACCAGCCTG
  TGTACCACAAGATCCAGGGGGATGACTGGGAGCCCCGGCCCCTGCGGGCGGCATCCCCGT
  TCAGGTCATCTGTCCAGGGTGCATCGAGCCGGGAGGGCTCACCAGCCAGGAGCAGCACGC
  CACTCCACTCCCCCTCGCCCATCCGTGTGCACACCGTGGTCGACAGGCCTCAGCAGCCCA
  TGACCCATCGAGAAACTGCACCTGTTTCCCAGCCTGAAAACAAACCAGAAAGTAAGCCAG
  GCCCAGTTGGACCAGAACTCCCTCCTGGACACATCCCAATTCAAGTGATCCGCAAAGAGG
  TGGATTCTAAACCTGTTTCCCAGAAGCCCCCACCTCCCTCTGAGAAGGTAGAGGTGAAAG
  TTCCCCCTGCTCCAGTTCCTTGTCCTCCTCCCAGCCCTGGCCCTTCTGCTGTCCCCTCTT
  CCCCCAAGAGTGTGGCTACAGAAGAGAGGGCAGCCCCCAGCACTGCCCCTGCAGAAGCTA
  CACCTCCAAAACCAGGAGAAGCCGAGGCTCCCCCAAAACATCCAGGAGTGCTGAAAGTGG
  AAGCCATCCTGGAGAAGGTACAGGGGCTGGAGCAGGCTGTAGACAACTTTGAAGGCAAGA
  AGACTGACAAAAAGTACCTGATGATCGAAGAGTATTTGACCAAAGAGCTGCTGGCCCTGG
  ATTCAGTGGACCCCGAGGGACGAGCCGATGTGCGTCAGGCCAGGAGAGACGGTGTCAGGA
  AGGTTCAGACCATCTTGGAAAAACTTGAACAGAAAGCCATTGATGTCCCAGGTCAAGTCC
  AGGTCTATGAACTCCAGCCCAGCAACCTTGAAGCAGATCAGCCACTGCAGGCAATCATGG
  AGATGGGTGCCGTGGCAGCAGACAAGGGCAAGAAAAATGCTGGAAATGCAGAAGATCCCC
  ACACAGAAACCCAGCAGCCAGAAGCCACAGCAGCAGCGACTTCAAACCCCAGCAGCATGA
  CAGACACCCCTGGTAACCCAGCAGCACCGTAGCCTCTGCCCTGTAAAAATCAGACTCGGA
  ACCGATGTGTGCTTTAGGGAATTTTAAGTTGCATGCATTTCAGAGACTTTAAGTCAGTTG
  GTTTTTATTAGCTGCTTGGTATGCAGTAACTTGGGTGGAGGCAAAACACTAATAAAAGGG
  CTAAAAAGGAAAATGATGCTTTTCTTCTATATTCTTACTCTGTACAAATAAAGAAGTTGC
  TTGTTGTTTGAGAAGTTTAACCCCGTTGCTTGTTGTTCTGCAGCCCTGTCTACTTGGGCA
  CCCCCACCACCTGTTAGCTGTGGTTGTGCACTGTCTTTTGTAGCTCTGGACTGGAGGGGT
  AGATGGGGAGTCAATTACCCATCACATAAATATGAAACATTTATCAGAAATGTTGCCATT
  TTAATGAGATGATTTTCTTCATCTCATAATTAAAATACCTGACTTTAGAGAGAGTAAAAT
  GTGCCAGGAGCCATAGGAATATCTGTATGTTGGATGACTTTAATGCTACATTTTAAAAAA
  AGAAAATAAAGTAATAATATAACTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_004281
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004281.3, NP_004272.2
• RefSeq Size: 2608 bp
• RefSeq ORF: 1728 bp
• Locus ID: 9531
• UniProt ID: O95817
• Cytogenetics: 10q26.11
• Domains: WW, BAG
• Protein Families: Druggable Genome
• Gene Summary: BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The protein encoded by this gene contains a WW domain in the N-terminal region and a BAG domain in the C-terminal region. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner. [provided by RefSeq, Jul 2008]