PNO1 (NM_020143) Human Untagged Clone

CAT#: SC320754

PNO1 (untagged)-Human partner of NOB1 homolog (S. cerevisiae) (PNO1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PNO1
• Synonyms: KHRBP1; RRP20
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_020143.2 TGGCTTCTGCGTGGTGCAGCTGCGCACGTGTTTCAGCCGGCAGCGCTTTAAGATTTCCGG
  GGATGGAATCCGAAATGGAAACGCAGAGCGCCAGGGCAGAGGAGGGCTTTACCCAGGTCA
  CCCGCAAGGGTGGCCGACGGGCGAAGAAACGACAGGCTGAACAGCTGTCCGCAGCAGGAG
  AGGGCGGGGATGCGGGCCGCATGGACACAGAGGAGGCCAGGCCGGCGAAGAGGCCCGTCT
  TCCCACCCCTCTGTGGGGACGGGCTCCTGAGTGGGAAAGAAGAAACAAGGAAAATTCCAG
  TCCCAGCTAACAGATACACACCATTGAAAGAAAACTGGATGAAGATATTTACTCCTATTG
  TGGAACATTTGGGACTTCAGATACGCTTTAACTTGAAATCAAGGAATGTAGAAATCAGGA
  CTTGTAAAGAAACCAAGGATGTTAGTGCTCTGACAAAAGCAGCTGATTTTGTGAAAGCTT
  TTATTCTCGGCTTTCAGGTGGAGGATGCACTTGCCCTCATCAGGTTGGATGACCTCTTCC
  TAGAGTCTTTTGAAATTACAGATGTTAAACCCCTAAAGGGAGACCATCTATCCAGGGCAA
  TAGGAAGAATCGCTGGCAAAGGAGGAAAAACCAAATTCACCATAGAGAATGTGACACGGA
  CAAGGATAGTTTTGGCTGATGTGAAAGTTCACATCCTTGGCTCCTTCCAAAATATCAAGA
  TGGCAAGAACTGCCATTTGCAACCTAATCTTGGGAAATCCTCCTTCCAAGGTTTATGGCA
  ATATTCGAGCTGTGGCTAGCAGATCAGCAGATCGATTCTGATTTCAAGTCAGAGACTTTT
  TATCTTGCCTTTGGACTCTGGTGAAAAATACTTTACAGTGGTCGGTCACAAGAAACCAGC
  TGAACAATTTCAGTCATTTGAAGCCTCCGTCCCTTCTTCCATTCTCAGCCAGAAGCATAA
  ACAGAAAAGAAAGATTTAAGAGGATTCACACTCAACAGGTTTTAGGATAATTTAAATATC
  AAAAATTGATTGTTATACTTAACACATTAGGTATAATTTATCATTTATCTGAAATCACAT
  GTAGCAGATTGCATAGTCTGTAATCCTCTCAGAGGGAAACTTCTTGTTTAAACAGCTCTA
  TATGGATTTATACTTTTATATTTATAAATTTATAACTTCATACAAATTTATAAACATTTC
  TTTATAAATTGTAATTTAATAGATTATCTCAGAAAAACCTCTCTGAATGATGACCCTTCC
  TTAATACTGGGTGATGTGTGAATATTTGTTTGTTGGCAGACAGGGTCTCACTTTGTCACC
  CAGGCTAGAGTGCAGTGGTGCAATCTCAGCTCACTGCAGCCTCTGCCTCCTGAGTTCAAG
  TGATCCTCCTGCTTCAGCCTCCCTAGTAGCTGGGACTACAGGTATGCACCACCATGCCTG
  GCTAATTTTTTGTATTTTTTGTAGAGATGGGGTTTCGCCATGTTGACCAGGCTGGTCTTG
  AACTCCTGGCCTCAAGGGATCCGTCCGCCTCAGCCTCCCAAAGTGCTGGGATTACAGGCA
  TAAGCCACTGCGCCTGGCCTTGATGTGTGAATATTTGAGAGGTCATAAGCAGTGGTTTTG
  GCCATACCGTATTATACCATATACATCAGTAAGAGCTCATCTTGGAACCTGTTTTGCAGT
  TTCTTGCAGTTTCAAAAATTAAAGACCTACATCACAGGGTTAATGTGAAGAAAGCTTTAT
  TTTTCAAATGAGTATTTAATGAAAGTATACATAACCAATGTTGGGTATACAGATGCTTCT
  CAACTTATGATGGGTTTAGGTCCAGATAAGCCCACTGTGAGTTGAAAATACCAAAAGTCA
  AACATCATAGCTTAGCCTACCTTAAAAGGGCTCCGAACACTACATTAGCCTACAGTTGGG
  CAAAATCGTCTAATACAAAGCCTATTTTATAATAAAGTATGTTGAATATCTCATGTAAAA
  AAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_020143
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020143.2, NP_064528.1
• RefSeq Size: 1994 bp
• RefSeq ORF: 759 bp
• Locus ID: 56902
• UniProt ID: Q9NRX1
• Cytogenetics: 2p14
• Domains: KH
• Gene Summary: Positively regulates dimethylation of two adjacent adenosines in the loop of a conserved hairpin near the 3'-end of 18S rRNA (PubMed:25851604).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.