RAB8A (NM_005370) Human Untagged Clone

CAT#: SC320702

RAB8A (untagged)-Human RAB8A, member RAS oncogene family (RAB8A)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RAB8A
• Synonyms: MEL; RAB8
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_005370.4 GCTCGGTGCTGATGGACGGTGCTGGTGGCCAGTGGAGAGGCGCTGGCCGCACTTCCCGTC
  GGGGAGAGAGTGTAATATGGCGAAGACCTACGATTACCTGTTCAAGCTGCTGCTGATCGG
  GGACTCGGGGGTGGGGAAGACCTGTGTCCTGTTCCGCTTCTCCGAGGACGCCTTCAACTC
  CACTTTTATCTCCACCATAGGAATTGACTTTAAAATTAGGACCATAGAGCTCGATGGCAA
  GAGAATTAAACTGCAGATATGGGACACAGCCGGTCAGGAACGGTTTCGGACGATCACAAC
  GGCCTACTACAGGGGTGCAATGGGCATCATGCTGGTCTACGACATCACCAACGAGAAGTC
  CTTCGACAACATCCGGAACTGGATTCGCAACATTGAGGAGCACGCCTCTGCAGACGTCGA
  AAAGATGATACTCGGGAACAAGTGTGATGTGAATGACAAGAGACAAGTTTCCAAGGAACG
  GGGAGAAAAGCTGGCCCTCGACTATGGAATCAAGTTCATGGAGACCAGCGCGAAGGCCAA
  CATCAATGTGGAAAATGCATTTTTCACTCTCGCCAGAGATATCAAAGCAAAAATGGACAA
  AAAATTGGAAGGCAACAGCCCCCAGGGGAGCAACCAGGGAGTCAAAATCACACCGGACCA
  GCAGAAGAGGAGCAGCTTTTTCCGATGTGTTCTTCTGTGAGGAACACCGCCTTACTCTGA
  GCCTCGCTCAGCCCAGCTGACTGTGCCTGTTCTGAGTGAGCCCCTCACTCAGCCGGGGCC
  CTCCCACCTCCAACGCCCCGCCCACGCCGCGGCCACCGGGCCCACGGCCACCAGAATGCA
  ATTGAGAAATCGTTTATTTTAGTAACTGTCTGATCTTTTTCAACTTTGGAGATGGAATAA
  GTTAAAAATTTGCTATTTTTCCTGTAACATCTGCTGAACGGGCCCACCCACACGTTGTAT
  ATTCAGAGAGAGAGAGGGAGTCAAGGTGTGACCGTCGACCACAGCCAGTGTCAGGCCTCT
  GCCTCTGGGCCTTTGCTTTGTGGCCTCACTGCAACACAAAGCTCCACCAGGAGGCTGGTT
  CACGTCCCCTACCACGGAAGCGAGGTCCCAGAAGGCCAGCGGTGGTTCCAGGAGCAACAG
  CTCCCAAACCCTGAGCAAGGCAACCGATCGCCAGGACCAGGAAGCATCACCCAGGAGATT
  TGGCGCCCACTTCCACCTCTTCTCTCAGTTTTGGACAAGTGACAAACCATTTTGCCCCCT
  CACTCTTCTTTTTTAACTGTTAAACCAAAGGAAAGCACAAATGAAGGAAATCCTGTGTAA
  AGCATTGAGAAGGAAAGAAGCCTGGAGCAGCCTCTCCTGTCCACAGCCAGGGGTTAGGTC
  TGCAGGCCCGTCTGCGGTCCCCATCGAGCATCAAGGGGACGCTGTGTGTGCTGCAAGTGA
  CCCCGAAAACAACCACAGCCGTCACATGGTCCTCCTGAAGTTGGGGCACCCTCCTCTCAG
  CACCAAAATGGCCCCCACTCCTTCGTGTCCTCCCGCTATCTCCAAATCGGACGTTCTTTC
  TAGCTGAGATTTTTATTTTTCCACATCTGTAGTGCCATGAAGCGATTCTGTCTTTGACTT
  CCAATGGCAAACCTGGGTGATCGGGAACAAGCACGTTGTACCCTTGGCTGGACATGGCCA
  AGACACAAGGCATTCCACGGCGGCAAGCTGACCGCACAGCAGTCTGGCTTGATTTTCAGC
  CGTCATCATTGGGTTCTGTTTTGACAGCTCTGCTGTCCCATAGGGACTGCGACTGGGACC
  AGGTCAACCACGCCAGGGGGGTGTCACCAGCCTTTTCTTTTTTTCTTTCTTTTTTTTTTT
  TTCCTCCTTAAGCTGCTGTCAATCCAAACCATTGGCATCATCGTTTCTTTTCAATTAAAA
  CCAACATATCAGCAATAGTCTGCTCTCCCCGGGAATCTCTAACATGCTCTGTTTACATCG
  ATAAATGCACTTAAGGAAAACAAACAAATTAAAGCTCATCTTAAAGTCCAAAAAAAAAAA
  AAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_005370
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005370.4, NP_005361.2
• RefSeq Size: 2194 bp
• RefSeq ORF: 624 bp
• Locus ID: 4218
• UniProt ID: P61006
• Cytogenetics: 19p13.11
• Domains: ras, RAN, RAS, RHO, RAB, ARF
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: The protein encoded by this gene is a member of the RAS superfamily which are small GTP/GDP-binding proteins with an average size of 200 amino acids. The RAS-related proteins of the RAB/YPT family may play a role in the transport of proteins from the endoplasmic reticulum to the Golgi and the plasma membrane. This protein shares 97%, 96%, and 51% similarity with the dog RAB8, mouse MEL, and mouse YPT1 proteins, respectively and contains the 4 GTP/GDP-binding sites that are present in all the RAS proteins. The putative effector-binding site of this protein is similar to that of the RAB/YPT proteins. However, this protein contains a C-terminal CAAX motif that is characteristic of many RAS superfamily members but which is not found in YPT1 and the majority of RAB proteins. Although this gene was isolated as a transforming gene from a melanoma cell line, no linkage between MEL and malignant melanoma has been demonstrable. This oncogene is located 800 kb distal to MY09B on chromosome 19p13.1. [provided by RefSeq, Jul 2008]