p38 (CRK) (NM_005206) Human Untagged Clone

CAT#: SC320373

CRK (untagged)-Human v-crk sarcoma virus CT10 oncogene homolog (avian) (CRK), transcript variant I

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: p38
• Synonyms: CRKII; p38
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_005206.3 GGGCACGAGGCTGCTGTGAAGCTGAAACCGGAGCCGGTCCGCTGGGCGGCGGGCGCCGGG
  GGCCGGAGGGGCGCGCGCGGCGGCGGCACCCCAGCGTTTAGGCGCGGAGGCAGCCATGGC
  GGGCAACTTCGACTCGGAGGAGCGGAGTAGCTGGTACTGGGGGAGGTTGAGTCGGCAGGA
  GGCGGTGGCGCTGCTGCAGGGCCAGCGGCACGGGGTGTTCCTGGTGCGGGACTCGAGCAC
  CAGCCCCGGGGACTATGTGCTCAGCGTCTCAGAGAACTCGCGCGTCTCCCACTACATCAT
  CAACAGCAGCGGCCCGCGCCCGCCGGTGCCACCGTCGCCCGCCCAGCCTCCGCCCGGGGT
  GAGCCCCTCCAGACTCCGAATAGGAGATCAAGAGTTTGATTCATTGCCTGCTTTACTGGA
  ATTCTACAAAATACACTATTTGGACACTACAACGTTGATAGAACCAGTTTCCAGATCCAG
  GCAGGGTAGTGGAGTGATTCTCAGGCAGGAGGAGGCGGAGTATGTGCGAGCCCTCTTTGA
  CTTTAATGGGAATGATGAGGAAGATCTTCCCTTTAAGAAAGGAGACATCTTGAGAATCCG
  GGACAAGCCTGAAGAGCAGTGGTGGAATGCGGAGGACAGCGAAGGCAAGAGAGGGATGAT
  TCCAGTCCCTTACGTCGAGAAGTATAGACCTGCCTCCGCCTCAGTATCGGCTCTGATTGG
  AGGTCGGTGAGCTGGTAAAGGTTACGAAGATTAATGTGAGTGGTCAGTGGGAAGGGGAGT
  GTAATGGCAAACGAGGTCACTTCCCATTCACACATGTCCGTCTGCTGGATCAACAGAATC
  CCGATGAGGACTTCAGCTGAGTATAGTTCAACAGTTTTGCTGACAGATGGGAACAATCTT
  TTTTTTTTTTTTTCCAACTGCCATCTATACAATTTTCTTACAGATGTCAAAAGCAGTCTA
  GTTTATATAAGCATTCTGTTACCTGTGATATTTTTTAGACTGAACTGCTCCATTCCTAGT
  CTTAATTACCATATTCAGGGTACGAACTGGAGGGCTTGTGTGTTAGCTTCTGAATTGGCA
  ATTGGAGGCGGTAGTGGTCGTGCCTGTGTGTATCAGAAGGGATAGGTATCTTGCCTCCTT
  TCTCTCAGGCAGTGCAAATCACCCTGTGGAAAACCGATGGACAGGAAGGAGTGTTACACA
  CTGCTTACCCTGATTTATTCAGTGGTTTTGTTTTCATTCTGGAACCATACTATCAAATGG
  CGACAGACTGTTCCGTTCCACCCCCGTGAAGTAATCATGCACCGTGTGAATAGTATCAAG
  CAGGATTGCTTTCATTGTATGGAGCATGACCAGCGTGTGACTCATTCTGACATTTCAGAT
  CCTAAGAATTCTAAGAACACTACTAGAAGCATTTGTTCCCTCCTAGTCAATGCTTCATAC
  TTTTTCTTGGGATTCTTTTAGCCCTTGACATTCTTGTCCCCCAAACCTGTAAGTAGGTGA
  ATTCCTAAGATAAGTGTGTATTTTCATTCCAGGTGAAAAGCAGGATGTACCGAGCACTTT
  ATTCAGTGCATAGCTTTAAGCCAGTGTTGGATTCACTAAGTGGACAGCCAGTCTCCCAGC
  TCTCTGCCTTCCCCAAAAGGGTCGTAGTAGGTCACCCTTCTACAGCAGCTAACTAGAGTC
  CTAACTAATGGGATCCAGCAGGGCCATTTCTCCAGAGGGCCAGTATCCTATTAGGAGACT
  CTTGGAATTCTTAGGTTCTACTCAAGAGTGGAAGGACCAATCACCTCTGATATTCTGTGG
  AAGGTTTTGGGGTCAAATTCTGCCCTCTGCATTCTGTGCAACTTGTATAAAAGTCAAGTT
  AGTATTACATGAATTTGGGGTAGGGTTAGTGCTTTGAAAAAATGTTGAACCGGCTGGGCG
  CGGTGGCTCACGTCTGTAATCCCAGCACTTTGGGAGGCCGAGGCGGGTGGATCATGAGGT
  CAGGAGTTCGAGACCAGCCTGGCCAACATAGTGAAACCCCATCTCTGCTAAAGATATAAA
  AAATTAGCCCGGCGTGGTGGTGCACGCCTGTAATCCCAGCTACTCGGGAGGCTGAGGCAG
  GAGAATTGCTTCAACCTGGGAGGTGGAGGCTGCAGTGAGCCGAGATCGCACCACTGCGTT
  CCAGCCTGAGCGACAGGGCAAGACTCAGTCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_005206
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005206.3, NP_005197.3
• RefSeq Size: 2245 bp
• RefSeq ORF: 615 bp
• Locus ID: 1398
• UniProt ID: P46108
• Cytogenetics: 17p13.3
• Domains: SH2, SH3
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Chemokine signaling pathway, Chronic myeloid leukemia, ErbB signaling pathway, Fc gamma R-mediated phagocytosis, Focal adhesion, Insulin signaling pathway, MAPK signaling pathway, Neurotrophin signaling pathway, Pathways in cancer, Regulation of actin cytoskeleton, Renal cell carcinoma
• Gene Summary: This gene encodes a member of an adapter protein family that binds to several tyrosine-phosphorylated proteins. The product of this gene has several SH2 and SH3 domains (src-homology domains) and is involved in several signaling pathways, recruiting cytoplasmic proteins in the vicinity of tyrosine kinase through SH2-phosphotyrosine interaction. The N-terminal SH2 domain of this protein functions as a positive regulator of transformation whereas the C-terminal SH3 domain functions as a negative regulator of transformation. Two alternative transcripts encoding different isoforms with distinct biological activity have been described. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (I) represents the shorter transcript and encodes the shorter isoform (b). Isoform b increases transformation activity due to the loss of a SH3 domain.