Spermine synthase (SMS) (NM_004595) Human Untagged Clone

CAT#: SC320292

SMS (untagged)-Human spermine synthase (SMS)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Spermine synthase
• Synonyms: MRSR; SPMSY; SpS; SRS
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_004595.2 CAGCCTGACACGCCGCGCGGCCCCCCAGTCTCCCGCGGCTGCTCCCCCAGGCATGGCACA
  GGGCCTCGCCTCACTATGGCAGCAGCACGGCACAGCACGCTCGACTTCATGCTCGGCGCC
  AAAGCTGATGGTGAGACCATTCTAAAAGGCCTCCAGTCCATTTTCCAGGAGCAGGGGATG
  GCGGAGTCGGTGCACACCTGGCAGGACCATGGCTATTTAGCAACCTACACAAACAAGAAC
  GGCAGCTTTGCCAATTTGAGAATTTACCCACATGGATTGGTGTTGCTGGACCTTCAGAGT
  TATGATGGTGATGCGCAAGGCAAAGAAGAGATCGACAGTATTTTGAACAAAGTAGAGGAA
  AGAATGAAAGAATTGAGTCAGGACAGTACTGGGCGGGTGAAACGATTACCACCCATAGTG
  CGAGGAGGAGCCATCGACAGATACTGGCCCACCGCCGACGGGCGCCTGGTTGAATATGAC
  ATAGATGAAGTGGTATATGACGAAGATTCACCTTATCAAAATATAAAAATTCTACACTCG
  AAGCAGTTTGGAAATATTCTCATCCTTAGTGGGGATGTTAATTTGGCAGAGAGTGATTTG
  GCATATACCCGGGCCATCATGGGCAGTGGCAAAGAAGATTACACTGGCAAAGATGTACTC
  ATTCTGGGAGGTGGAGACGGAGGCATATTGTGTGAAATAGTCAAACTAAAACCAAAGATG
  GTCACTATGGTAGAGATTGACCAAATGGTGATTGATGGGTGTAAGAAATACATGCGAAAA
  ACGTGTGGCGATGTCTTAGACAATCTTAAAGGAGACTGCTATCAGGTTCTAATAGAAGAC
  TGTATCCCGGTACTGAAGAGGTACGCCAAAGAAGGGAGAGAATTTGATTATGTGATTAAT
  GATTTGACAGCTGTTCCAATCTCCACGTCTCCAGAAGAAGATTCCACATGGGAGTTTCTC
  AGACTGATTCTTGACCTCTCAATGAAAGTGTTGAAACAGGATGGGAAATATTTTACACAG
  GGGAACTGTGTCAATCTGACAGAAGCACTGTCGCTCTATGAAGAACAGCTGGGGCGCCTG
  TATTGTCCTGTGGAATTTTCAAAGGAGATCGTCTGTGTCCCTTCATACTTGGAATTGTGG
  GTATTTTACACTGTTTGGAAGAAAGCTAAACCCTGAAGATCAGTAGCCCCTAATCACATG
  TGCTGCAAATAGCCTTCCTGACCTCCATATGCTGTACATGACATCAAAATGAGTCAGGCA
  ATTGATTGTGAATTCCTTAAAGTTTTCCTTTTTTTAATAATTATTTTTAATTTAAAAAAG
  CAAATGGAAAATGTATATTTTGATGAGCTTAGGGTGTTTTTTTTTTTGAAAGTCAGCTGA
  AGGATGGTTAGACAGCACAGCGAAGACTGCTAAATGCACTGACCCCCCCCATTAGAATGT
  GATTTTTGTTCCTTTTTATTTCTCTGTGGGCTTTTGTTTTTGTTTTTGTTTTGGTAGATC
  TTCAATTTGGATATTTGGAGGAGTGAACATCGTTGTTTTGCTGGAGGGAAGATCTTGATG
  GTGTTTCTTTCCCCAAAAATTGACTTAGATATTAAAATTTGGTGCTTATAAGAGAGAGTT
  AAAAAAAAATAGGATTGCTTCAATTAAAATTACAAAAGAGACAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_004595
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004595.2, NP_004586.2
• RefSeq Size: 1717 bp
• RefSeq ORF: 1101 bp
• Locus ID: 6611
• UniProt ID: P52788
• Cytogenetics: Xp22.11
• Domains: Spermine_synth
• Protein Pathways: Arginine and proline metabolism, beta-Alanine metabolism, Cysteine and methionine metabolism, Glutathione metabolism, Metabolic pathways
• Gene Summary: This gene encodes a protein belonging to the spermidine/spermin synthase family and catalyzes the production of spermine from spermidine. Pseudogenes of this gene are located on chromosomes 1, 5, 6 and X. Mutations in this gene cause an X-linked intellectual disability called Snyder-Robinson Syndrome (SRS). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2017]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer protein (isoform 1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.