PGD (NM_002631) Human Untagged Clone

CAT#: SC320261

PGD (untagged)-Human phosphogluconate dehydrogenase (PGD)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PGD
• Synonyms: 6PGD
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_002631.2 CGCGTCGCCGCTCTTCGGTTCTGCTCTGTCCGCCGCCATGGCCCAAGCTGACATCGCGCT
  GATCGGATTGGCCGTCATGGGCCAGAACTTAATTCTGAACATGAATGACCACGGCTTTGT
  GGTCTGTGCTTTTAATAGGACTGTCTCCAAAGTTGATGATTTCTTGGCCAATGAGGCAAA
  GGGAACCAAAGTGGTGGGTGCCCAGTCCCTGAAAGAGATGGTCTCCAAGCTGAAGAAGCC
  CCGGCGGATCATCCTCCTGGTGAAGGCTGGGCAAGCTGTGGATGATTTCATCGAGAAATT
  GGTACCATTGTTGGATACTGGTGACATCATCATTGACGGAGGAAATTCTGAATATAGGGA
  CACCACAAGACGGTGCCGAGACCTCAAGGCCAAGGGAATTTTATTTGTGGGGAGCGGAGT
  CAGTGGTGGAGAGGAAGGGGCCCGGTATGGCCCATCGCTCATGCCAGGAGGGAACAAAGA
  AGCGTGGCCCCACATCAAGACCATCTTCCAAGGCATTGCTGCAAAAGTGGGAACTGGAGA
  ACCCTGCTGTGACTGGGTGGGAGATGAGGGAGCAGGCCACTTCGTGAAGATGGTGCACAA
  CGGGATAGAGTATGGGGACATGCAGCTGATCTGTGAGGCATACCACCTGATGAAAGACGT
  GCTGGGCATGGCGCAGGACGAGATGGCCCAGGCCTTTGAGGATTGGAATAAGACAGAGCT
  AGACTCATTCCTGATTGAAATCACAGCCAATATTCTCAAGTTCCAAGACACCGATGGCAA
  ACACCTGCTGCCAAAGATCAGGGACAGCGCGGGGCAGAAGGGCACAGGGAAGTGGACCGC
  CATCTCCGCCCTGGAATACGGCGTACCCGTCACCCTCATTGGAGAAGCTGTCTTTGCTCG
  GTGCTTATCATCTCTGAAGGATGAGAGAATTCAAGCTAGCAAAAAGCTGAAGGGTCCCCA
  GAAGTTCCAGTTTGATGGTGATAAGAAATCATTCCTGGAGGACATTCGGAAGGCACTCTA
  CGCTTCCAAGATCATCTCTTACGCTCAAGGCTTTATGCTGCTAAGGCAGGCAGCCACCGA
  GTTTGGCTGGACTCTCAATTATGGTGGCATCGCCCTGATGTGGAGAGGGGGCTGCATCAT
  TAGAAGTGTATTCCTAGGAAAGATAAAGGATGCATTTGATCGAAACCCGGAACTTCAGAA
  CCTCCTACTGGACGACTTCTTTAAGTCAGCTGTTGAAAACTGCCAGGACTCCTGGCGGCG
  GGCAGTCAGCACTGGGGTCCAGGCTGGCATTCCCATGCCCTGTTTTACCACTGCCCTCTC
  CTTCTATGACGGGTACAGACATGAGATGCTTCCAGCCAGCCTCATCCAGGCTCAGCGGGA
  TTACTTCGGGGCTCACACCTATGAACTCTTGGCCAAACCAGGGCAGTTTATCCACACCAA
  CTGGACAGGCCATGGTGGCACCGTGTCATCCTCGTCATACAATGCCTGATCATGCTGCTC
  CTGTCACCCTCCACGATTCCACAGACCAGGACATTCCATGTGCCTCATGGCACTGCCACC
  TGGCCCTTTGCCCTATTTTCTGTTCAGTTTTTTAAAAGTGTTGTAAGAGACTCCTGAGGA
  AGACACACAGTTTATTTGTAAAGTAGCTCTGTGAGAGCCACCATGCCCTCTGCCCTTGCC
  TCTTGGGACTGACCAGGAGCTGCTCATGTGCGTGAGAGTGGGAACCATCTCCTTGCGGCA
  GTGGCTTCCGCGTGCCCCGTGTGCTGGTGCGGTTCCCATCACGCAGACAGGAAGGGTGTT
  TGCGCACTCTGATCAACTGGAACCTCTGTATCATGCGGCTGAATTCCCTTTTTCCTTTAC
  TCAATAAAAGCTACATCACACTGAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_002631
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002631.2, NP_002622.2
• RefSeq Size: 1937 bp
• RefSeq ORF: 1452 bp
• Locus ID: 5226
• UniProt ID: P52209
• Cytogenetics: 1p36.22
• Domains: 6PGD, NAD_binding_2
• Protein Pathways: Glutathione metabolism, Metabolic pathways, Pentose phosphate pathway
• Gene Summary: 6-phosphogluconate dehydrogenase is the second dehydrogenase in the pentose phosphate shunt. Deficiency of this enzyme is generally asymptomatic, and the inheritance of this disorder is autosomal dominant. Hemolysis results from combined deficiency of 6-phosphogluconate dehydrogenase and 6-phosphogluconolactonase suggesting a synergism of the two enzymopathies. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2015]
  Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.