ARD1A (NAA10) (NM_003491) Human Untagged Clone

CAT#: SC320239

NAA10 (untagged)-Human N(alpha)-acetyltransferase 10, NatA catalytic subunit (NAA10)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ARD1A
• Synonyms: ARD1; ARD1A; ARD1P; DXS707; hARD1; MCOPS1; NATD; OGDNS; TE2
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_003491.2 AGCGAGCCCAGCTCCGGTCCCAGCCCCGGCCGTCCCGGCGTCGCTTCGGAGCGCGGCGGC
  AGCTGACTGCGCCTTCACGATCCGCTGGGACCCGCGAGCCCCGCCGCCGTTATGAACATC
  CGCAATGCGAGGCCAGAGGACCTAATGAACATGCAGCACTGCAACCTCCTCTGCCTGCCC
  GAGAACTACCAGATGAAATACTACTTCTACCATGGCCTTTCCTGGCCCCAGCTCTCTTAC
  ATTGCTGAGGACGAGAATGGGAAGATTGTGGGGTATGTCCTGGCCAAAATGGAAGAGGAC
  CCAGATGATGTGCCCCATGGACATATCACCTCATTGGCTGTGAAGCGTTCCCACCGGCGC
  CTCGGTCTGGCTCAGAAACTGATGGACCAGGCCTCTCGAGCCATGATAGAGAACTTCAAT
  GCCAAATATGTCTCCCTGCATGTCAGGAAGAGTAACCGGGCCGCCCTGCACCTCTATTCC
  AACACCCTCAACTTTCAGATCAGTGAAGTGGAGCCCAAATACTATGCAGATGGGGAGGAC
  GCCTATGCCATGAAGCGGGACCTCACTCAGATGGCCGACGAGCTGAGGCGGCACCTGGAG
  CTGAAAGAGAAGGGCAGGCACGTGGTGCTGGGTGCCATCGAGAACAAGGTGGAGAGCAAA
  GGCAATTCACCTCCGAGCTCAGGAGAGGCCTGTCGCGAGGAGAAGGGCCTGGCTGCCGAG
  GATAGTGGTGGGGACAGCAAGGACCTCAGCGAGGTCAGCGAGACCACAGAGAGCACAGAT
  GTCAAGGACAGCTCAGAGGCCTCCGACTCAGCCTCCTAGAGCCTGCCCCATCCCCTCCTC
  ACCCCACGAGCTTTCACAATAAATTTGCTCCGTGGCACTGGGAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_003491
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003491.2, NP_003482.1
• RefSeq Size: 916 bp
• RefSeq ORF: 708 bp
• Locus ID: 8260
• UniProt ID: P41227
• Cytogenetics: Xq28
• Domains: Acetyltransf
• Protein Families: Druggable Genome
• Protein Pathways: Glycerophospholipid metabolism, Limonene and pinene degradation, Phenylalanine metabolism, Tyrosine metabolism
• Gene Summary: N-alpha-acetylation is among the most common post-translational protein modifications in eukaryotic cells. This process involves the transfer of an acetyl group from acetyl-coenzyme A to the alpha-amino group on a nascent polypeptide and is essential for normal cell function. This gene encodes an N-terminal acetyltransferase that functions as the catalytic subunit of the major amino-terminal acetyltransferase A complex. Mutations in this gene are the cause of Ogden syndrome. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2012]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).