GRHPR (NM_012203) Human Untagged Clone

CAT#: SC320204

GRHPR (untagged)-Human glyoxylate reductase/hydroxypyruvate reductase (GRHPR)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GRHPR
• Synonyms: GLXR; GLYD; PH2
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_012203.1 GGTCGGCGGCTGCACTGCGGATGAGACCGGTGCGACTCATGAAGGTGTTCGTCACCCGCA
  GGATACCCGCCGAGGGTAGGGTCGCGCTCGCCCGGGCGGCAGACTGTGAGGTGGAGCAGT
  GGGACTCGGATGAGCCCATCCCTGCCAAGGAGCTAGAGCGAGGTGTGGCGGGGGCCCACG
  GCCTGCTCTGCCTCCTCTCCGACCACGTGGACAAGAGGATCCTGGATGCTGCAGGGGCCA
  ATCTCAAAGTCATCAGCACCATGTCTGTGGGCATCGACCACTTGGCTTTGGATGAAATCA
  AGAAGCGTGGGATCCGAGTTGGCTACACCCCAGATGTCCTGACAGATACCACCGCCGAAC
  TCGCAGTCTCCCTGCTACTTACCACCTGCCGCCGGTTGCCGGAGGCCATCGAGGAAGTGA
  AGAATGGTGGCTGGACCTCGTGGAAGCCCCTCTGGCTGTGTGGCTATGGACTCACGCAGA
  GCACTGTCGGCATCATCGGGCTGGGGCGCATAGGCCAGGCCATTGCTCGGCGTCTGAAAC
  CATTCGGTGTCCAGAGATTTCTGTACACAGGGCGCCAGCCCAGGCCTGAGGAAGCAGCGG
  AATTCCAGGCAGAGTTTGTGTCTACCCCTGAGCTGGCTGCCCAATCTGATTTCATCGTCG
  TGGCCTGCTCCTTAACACCTGCAACCGAGGGACTCTGCAACAAGGACTTCTTCCAGAAGA
  TGAAGGAAACAGCTGTGTTCATCAACATCAGCAGGGGCGACGTCGTAAACCAGGACGACC
  TGTACCAGGCCTTGGCCAGTGGTAAGATTGCAGCTGCTGGACTGGATGTGACGAGCCCAG
  AACCACTGCCTACAAACCACCCTCTCCTGACCCTGAAGAACTGTGTGATTCTGCCCCACA
  TTGGCAGTGCCACCCACAGAACCCGCAACACCATGTCCTTGTTGGCAGCTAACAACTTGC
  TGGCTGGCCTGAGAGGGGAGCCGATGCCTAGTGAACTCAAGCTGTAGCCAAACAGTAGAG
  ATGGAGGGCCGGGAAGCAAACCGTGCCCTGGTATTGTCAGACACACCCAGGCTTGATTTG
  GATCCACAGGCAGAGCCAAGGGAAGGTGTGATTCTCTGAGGAAAGAGTGATTCTGATATA
  TGTACTTGTCACATTGGTGTTGGACACATTTGCGCCAAAAGTATGGTAATTCTATTATTA
  AATAATTCTCTGAGAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_012203
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012203.1, NP_036335.1
• RefSeq Size: 1235 bp
• RefSeq ORF: 987 bp
• Locus ID: 9380
• UniProt ID: Q9UBQ7
• Cytogenetics: 9p13.2
• Domains: 2-Hacid_DH, 2-Hacid_DH_C
• Protein Families: Druggable Genome
• Protein Pathways: Glyoxylate and dicarboxylate metabolism, Metabolic pathways, Pyruvate metabolism
• Gene Summary: This gene encodes an enzyme with hydroxypyruvate reductase, glyoxylate reductase, and D-glycerate dehydrogenase enzymatic activities. The enzyme has widespread tissue expression and has a role in metabolism. Type II hyperoxaluria is caused by mutations in this gene. [provided by RefSeq, Jul 2008]