PHKA2 (NM_000292) Human Untagged Clone

CAT#: SC319895

PHKA2 (untagged)-Human phosphorylase kinase, alpha 2 (liver) (PHKA2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PHKA2
• Synonyms: GSD9A; PHK; PYK; PYKL; XLG; XLG2
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000292.1 CGCGCCTCCCCGCCGCGCGGAGCTCTGGTTGGCTTGCTTTCCAACCGGACTTTGGGGCTA
  GCGTTTAGAAAAGTCTCGACCTCCTCCGGCCCGGTCCCATCCCAAGAACCGACTAAGGCT
  GTGAGTGTCCGGGAACCAGACCCGCTTGGAGGCCACAGCCCCGACGTCCCGCGCCCACGC
  GGCAGATCGGGCGCTGCGGCCTGGGAGCCTCGGGGAGATGCGGAGCAGGAGCAATTCCGG
  GGTCCGCTTGGACGGGTACGCGCGGCTGGTGCAGCAAACCATCCTGTGTTACCAGAATCC
  CGTCACGGGGCTGCTGTCAGCCAGCCATGAGCAGAAGGATGCCTGGGTGCGGGATAACAT
  CTACAGTATCCTGGCCGTGTGGGGCCTGGGCATGGCCTACCGTAAGAATGCAGACCGCGA
  TGAGGACAAGGCCAAGGCCTACGAGCTGGAGCAGAACGTGGTGAAGCTGATGCGAGGTCT
  TCTCCAGTGCATGATGAGACAGGTGGCCAAAGTGGAGAAGTTCAAACACACTCAGAGCAC
  CAAGGACAGCCTGCACGCCAAGTACAACACCGCCACCTGTGGCACGGTGGTGGGCGACGA
  CCAGTGGGGCCACCTCCAGGTGGATGCCACCTCTCTCTTCCTCCTGTTCCTGGCCCAGAT
  GACCGCCTCAGGCTTACGTATCATTTTCACTCTCGATGAGGTGGCCTTCATACAGAATCT
  TGTCTTTTACATAGAAGCTGCATATAAAGTCGCTGATTATGGAATGTGGGAGCGTGGAGA
  TAAGACTAATCAGGGCATCCCGGAATTGAATGCAAGCTCCGTAGGAATGGCCAAGGCAGC
  TCTTGAGGCAATTGATGAACTGGACCTTTTTGGAGCCCATGGAGGACGCAAGTCAGTGAT
  TCATGTTCTGCCAGATGAGGTCGAGCACTGCCAGTCTATTCTGTTCTCCATGCTGCCAAG
  AGCGTCGACATCTAAAGAAATTGATGCTGGACTTCTTTCCATTATTTCCTTCCCGGCCTT
  TGCAGTGGAAGATGTAAACCTTGTAAATGTGACCAAAAATGAAATTATTTCTAAGCTCCA
  GGGGCGTTATGGATGCTGTCGCTTCCTTCGAGATGGTTATAAAACTCCAAGAGAGGACCC
  TAATCGACTGCATTATGACCCTGCTGAACTCAAGCTCTTCGAAAACATTGAATGTGAGTG
  GCCTGTGTTTTGGACATATTTTATAATAGATGGAGTCTTCAGTGGTGATGCTGTTCAGGT
  CCAAGAATACCGAGAGGCCCTGGAGGGAATACTCATCAGAGGCAAGAATGGGATCCGCCT
  GGTGCCTGAACTCTACGCTGTCCCGCCTAACAAGGTAGATGAAGAGTACAAGAATCCTCA
  CACAGTAGACCGAGTTCCTATGGGGAAGGTGCCTCATCTGTGGGGCCAATCCTTGTACAT
  CCTCAGCTCGCTGTTGGCAGAGGGATTCCTTGCCGCTGGTGAAATCGATCCCTTAAATAG
  AAGATTTTCCACTTCAGTCAAACCTGATGTTGTAGTACAAGTTACTGTTTTGGCAGAAAA
  CAATCACATTAAGGACTTATTGAGGAAACACGGGGTGAACGTCCAGAGTATCGCGGACAT
  TCATCCAATTCAAGTCCAGCCGGGCCGGATTCTTAGTCACATATATGCCAAGCTTGGACG
  GAATAAGAATATGAATTTGAGTGGGCGACCGTATCGACATATTGGTGTCCTTGGAACCTC
  TAAACTATATGTGATTAGGAACCAAATCTTTACTTTTACACCCCAGTTCACCGACGAGCA
  TCACTTCTACCTGGCCCTCGACAATGAGATGATCGTGGAGATGCTAAGGATCGAGCTGGC
  CTACCTGTGCACCTGCTGGAGGATGACGGGCAGACCCACACTCACCTTCCCCATCAGTCG
  CACCATGCTCACAAATGATGGCTCAGACATTCATTCTGCTGTGCTCTCCACAATTAGAAA
  ACTAGAGGATGGATATTTTGGAGGAGCCAGAGTAAAATTAGGGAACCTTTCGGAATTTCT
  CACCACATCGTTCTACACATATCTGACTTTTCTGGATCCAGACTGTGATGAGAAGTTGTT
  TGACAATGCCAGCGAAGGGACTTTCAGTCCTGATAGTGATTCAGATTTGGTAGGATATCT
  GGAAGACACCTGTAATCAAGAAAGCCAAGACGAACTTGACCATTATATCAACCACCTTCT
  GCAAAGCACATCGTTGAGGTCCTATCTGCCTCCTCTTTGTAAGAACACAGAAGACCGCCA
  TGTCTTCAGTGCTATCCACTCCACGCGGGACATACTTTCTGTGATGGCAAAAGCAAAGGG
  TTTGGAAGTTCCATTTGTTCCCATGACTTTGCCGACTAAAGTTCTAAGTGCCCACCGTAA
  ATCACTGAATCTTGTTGATTCTCCTCAGCCACTCCTAGAAAAGGTTCCTGAAAGTGACTT
  TCAGTGGCCCAGAGATGACCATAGTGACGTGGACTGTGAGAAGCTGGTTGAGCAGCTAAA
  AGATTGTTCGAACCTACAGGACCAAGCAGACATTCTGTACATTCTTTATGTCATAAAGGG
  TCCCAGCTGGGACACAAATCTCTCTGGACAGCACGGGGTCACCGTTCAAAACCTTCTTGG
  TGAGCTCTATGGGAAAGCCGGCTTGAACCAGGAGTGGGGTCTGATTCGCTACATCTCAGG
  CCTTCTCAGGAAGAAAGTGGAGGTCCTGGCTGAGGCCTGCACAGACCTGCTTTCGCACCA
  GAAGCAGCTCACCGTGGGCCTGCCGCCCGAGCCCCGGGAGAAGATCATCTCTGCGCCCCT
  TCCCCCAGAGGAGCTCACAAAACTCATCTACGAGGCCAGTGGGCAGGACATCAGCATTGC
  CGTCCTCACGCAGGAGATTGTGGTTTACCTGGCCATGTATGTCAGGGCGCAGCCCAGCCT
  CTTTGTGGAGATGCTGAGACTCCGGATTGGACTGATCATTCAGGTGATGGCCACGGAGCT
  GGCACGGAGCCTGAACTGCTCAGGAGAAGAGGCTTCTGAAAGTTTGATGAACCTCAGCCC
  TTTCGATATGAAAAATCTCCTGCACCATATTCTAAGTGGGAAAGAGTTTGGCGTTGAAAG
  AAGTGTGCGCCCTATCCACTCCTCCACATCCAGCCCTACCATCTCCATCCACGAGGTGGG
  CCATACCGGAGTCACCAAAACTGAGAGGAGTGGCATTAACAGACTGAGGAGTGAAATGAA
  ACAGATGACTAGGCGGTTTAGTGCTGATGAACAGTTCTTTTCTGTGGGCCAGGCCGCGTC
  CAGCAGTGCGCATTCCTCCAAGTCTGCGAGGTCCAGCACCCCATCCTCGCCCACTGGCAC
  GTCATCCTCAGACTCGGGAGGACATCACATCGGCTGGGGTGAGCGGCAGGGCCAGTGGCT
  GCGCAGGAGAAGGCTGGATGGGGCCATCAACAGGGTCCCCGTGGGATTCTACCAGAGGGT
  GTGGAAGATCCTCCAGAAGTGCCACGGTCTCTCCATCGATGGTTATGTCCTCCCATCCTC
  GACGACCCGAGAGATGACCCCGCATGAGATCAAGTTTGCTGTCCATGTCGAATCGGTGCT
  GAACCGCGTGCCGCAGCCCGAGTACCGGCAGCTGCTGGTGGAAGCCATCATGGTGCTGAC
  GCTGCTCTCGGACACGGAGATGACCAGCATCGGGGGCATCATCCACGTGGACCAGATCGT
  GCAGATGGCCAGTCAGCTGTTCTTGCAGGACCAGGTGTCAATTGGTGCCATGGACACCCT
  GGAGAAAGACCAAGCCACAGGAATCTGCCACTTCTTTTATGACAGCGCTCCGAGTGGGGC
  TTATGGGACGATGACCTACCTAACAAGAGCAGTGGCTTCTTATTTGCAGGAATTGTTGCC
  CAATTCGGGCTGCCAGATGCAATAGGGTCTCACCTGGAAACATGATCACACTCTCAATCT
  GTCACGTGCCCCCTAGCCTTACTGGGAACCTTCTGTCCCCCAAGATCCCCTGTGCTATCA
  GGAAAGCATGTCCCATCAGAAACACTCTCGGGGGGCAATGGTAGCACTCACCCTGAAACT
  GATGTATGTTAAAGCCACAGAGATAGAGCTGAGGAGTCCTGTGTTCCCCCGCAAGGAGCA
  CCCCGGGATCATTTTCTAGGTTCATTTCTCTGGAACATTTGCTGTAGCATCTGGTCTCAC
  GGACTCTGAGGAGGAATTGGAAATTGGTCTCTTTTGAGTGCAGAGGGAACTGAGACGCCA
  GCTTAAATTGGCTCTTGCAGAGAGTTACAGAAATAGTTTCGATGAGCTAGTGACACATCC
  TAAAGATGCAAAGATCCTCCTGGCGGCAGTAGCCTTGACAAGGGCCACCTCTTCACAGGA
  TGCAGTCTGTCTGTGCACCAAACTCTTCACCAAATAGAACACTTGTGTCTCTCTGTGAAG
  AAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_000292
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000292.1, NP_000283.1
• RefSeq Size: 4566 bp
• RefSeq ORF: 3708 bp
• Locus ID: 5256
• UniProt ID: P46019
• Cytogenetics: Xp22.13
• Protein Families: Druggable Genome
• Protein Pathways: Calcium signaling pathway, Insulin signaling pathway
• Gene Summary: Phosphorylase kinase is a polymer of 16 subunits, four each of alpha, beta, gamma and delta. The alpha subunit includes the skeletal muscle and hepatic isoforms, and the hepatic isoform is encoded by this gene. The beta subunit is the same in both the muscle and hepatic isoforms, and encoded by one gene. The gamma subunit also includes the skeletal muscle and hepatic isoforms, which are encoded by two different genes. The delta subunit is a calmodulin and can be encoded by three different genes. The gamma subunits contain the active site of the enzyme, whereas the alpha and beta subunits have regulatory functions controlled by phosphorylation. The delta subunit mediates the dependence of the enzyme on calcium concentration. Mutations in this gene cause glycogen storage disease type 9A, also known as X-linked liver glycogenosis. Alternatively spliced transcript variants have been reported, but the full-length nature of these variants has not been determined.[provided by RefSeq, Feb 2010]