Fetuin A (AHSG) (NM_001622) Human Untagged Clone

CAT#: SC319889

AHSG (untagged)-Human alpha-2-HS-glycoprotein (AHSG)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Fetuin A
• Synonyms: A2HS; AHS; APMR1; FETUA; HSGA
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001622.1 CCTGGGTTGGTCCCGAAGCCTCCAACCACCTGCACGCCTGCCAGGGCCTCTCTGGGGCAG
  CCATGAAGTCCCTCGTCCTGCTCCTTTGTCTTGCTCAGCTGTGGGGCTGCCACTCAGCCC
  CACATGGCCCAGGGCTGATTTATAGACAACCGAACTGCGATGATCCAGAAACTGAGGAAG
  CAGCTCTGGTGGCTATAGACTACATCAATCAAAACCTTCCTTGGGGATACAAACACACCT
  TGAACCAGATTGATGAAGTAAAGGTGTGGCCTCAGCAGCCCTCCGGAGAGCTGTTTGAGA
  TTGAAATAGACACCCTGGAAACCACCTGCCATGTGCTGGACCCCACCCCTGTGGCAAGAT
  GCAGCGTGAGGCAGCTGAAGGAGCATGCTGTCGAAGGAGACTGTGATTTCCAGCTGTTGA
  AACTAGATGGCAAGTTTTCCGTGGTATACGCAAAATGTGATTCCAGTCCAGACTCAGCCG
  AGGACGTGCGCAAGGTGTGCCAAGACTGCCCCCTGCTGGCCCCGCTGAACGACACCAGGG
  TGGTGCACGCCGCGAAAGCTGCCCTGGCCGCCTTCAACGCTCAGAACAACGGCTCCAATT
  TTCAGCTGGAGGAAATTTCCCGGGCTCAGCTTGTGCCCCTCCCACCTTCTACCTATGTGG
  AGTTTACAGTGTCTGGCACTGACTGTGTTGCTAAAGAGGCCACAGAGGCAGCCAAGTGTA
  ACCTGCTGGCAGAAAAGCAATATGGCTTTTGTAAGGCAACACTCAGTGAGAAGCTTGGTG
  GGGCAGAGGTTGCAGTGACCTGCACGGTGTTCCAAACACAGCCCGTGACCTCACAGCCCC
  AACCAGAAGGTGCCAATGAAGCAGTCCCCACCCCCGTGGTGGACCCAGATGCACCTCCGT
  CCCCTCCACTTGGCGCACCTGGACTCCCTCCAGCTGGCTCACCCCCAGACTCCCATGTGT
  TACTGGCAGCTCCTCCAGGACACCAGTTGCACCGGGCGCACTACGACCTGCGCCACACCT
  TCATGGGTGTGGTCTCATTGGGGTCACCCTCAGGAGAAGTGTCGCACCCCCGGAAAACAC
  GCACAGTGGTGCAGCCTAGTGTTGGTGCTGCTGCTGGGCCAGTGGTTCCTCCATGTCCGG
  GGAGGATCAGACACTTCAAGGTCTAGGCTAGACATGGCAGAGATGAGGAGGTTTGGCACA
  GAAAACATAGCCACCATTTTGTCCAAGCCTGGGCATGGGTGGGGGGCCTTGTCTGCTGGC
  CACGCAAGTGTCACATGCGATCTACATTAATATCAAGTCTTGACTCCCTACTTCCTGTCA
  TTCCTCACAGGACAGAAGCAGAGTGGGTGGTGGTTATGTTTGACAGAAGGCATTAGGTTG
  ACAACTTGTCATGATTTTGACGGTAAGCCACCATGATTGTGTTCTCTGCCTCTGGTTGAC
  CTTACAAAAACCATTGGAACTGTGACTTTGAAAGGTGCTCTTGCTAAGCTTATATGTGCC
  TGTTAATGAAAGTGCCTGAAAGACCTTCCTTAATAAAGAAGGTTCTAAGCTGAAAAAAAA
  AAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_001622
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001622.1, NP_001613.1
• RefSeq Size: 1538 bp
• RefSeq ORF: 1104 bp
• Locus ID: 197
• UniProt ID: P02765
• Cytogenetics: 3q27.3
• Domains: CY
• Protein Families: Druggable Genome, Secreted Protein
• Gene Summary: The protein encoded by this gene is a negatively-charged serum glycoprotein that is synthesized by hepatocytes. The encoded protein consists of two polypeptide chains, which are both cleaved from a proprotein encoded from a single mRNA. It is involved in several processes, including endocytosis, brain development, and the formation of bone tissue. Defects in this gene are a cause of susceptibility to leanness. [provided by RefSeq, Aug 2017]
  Transcript Variant: This variant (2) uses an alternate in-frame splice junction compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is 1 aa shorter compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.