DUSP5 (NM_004419) Human Untagged Clone

CAT#: SC319809

DUSP5 (untagged)-Human dual specificity phosphatase 5 (DUSP5)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DUSP5
• Synonyms: DUSP; HVH3
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_004419.3 AGAGAGAGAACGCGGAGTTGCGCCGCCGCTCGGGCGCCGGGCTCCGTCGCGGCCGCAGCC
  CCGCGGGTCGCCCTCCCGTGCCTCGCCCGCGGACACCCTGGCCGTGGACACCCTGGCCGT
  GGGCACCCGCGGGGCGCGCGGCGCGGGGCCGCTGGCCGGCGGCGGCGGCGGCATGAAGGT
  CACGTCGCTCGACGGGCGCCAGCTGCGCAAGATGCTCCGCAAGGAGGCGGCGGCGCGCTG
  CGTGGTGCTCGACTGCCGGCCCTATCTGGCCTTCGCTGCCTCGAACGTGCGCGGCTCGCT
  CAACGTCAACCTCAACTCGGTGGTGCTGCGGCGGGCCCGGGGCGGCGCGGTGTCGGCGCG
  CTACGTGCTGCCCGACGAGGCGGCGCGCGCGCGGCTCCTGCAGGAGGGCGGCGGCGGCGT
  CGCGGCCGTGGTGGTGCTGGACCAGGGCAGCCGCCACTGGCAGAAGCTGCGAGAGGAGAG
  CGCCGCGCGTGTCGTCCTCACCTCGCTACTCGCTTGCCTACCCGCCGGCCCGCGGGTCTA
  CTTCCTCAAAGGGGGATATGAGACTTTCTACTCGGAATATCCTGAGTGTTGCGTGGATGT
  AAAACCCATTTCACAAGAGAAGATTGAGAGTGAGAGAGCCCTCATCAGCCAGTGTGGAAA
  ACCAGTGGTAAATGTCAGCTACAGGCCAGCTTATGACCAGGGTGGCCCAGTTGAAATCCT
  TCCCTTCCTCTACCTTGGAAGTGCCTACCATGCATCCAAGTGCGAGTTCCTCGCCAACTT
  GCACATCACAGCCCTGCTGAATGTCTCCCGACGGACCTCCGAGGCCTGCATGACCCACCT
  ACACTACAAATGGATCCCTGTGGAAGACAGCCACACGGCTGACATTAGCTCCCACTTTCA
  AGAAGCAATAGACTTCATTGACTGTGTCAGGGAAAAGGGAGGCAAGGTCCTGGTCCACTG
  TGAGGCTGGGATCTCCCGTTCACCCACCATCTGCATGGCTTACCTTATGAAGACCAAGCA
  GTTCCGCCTGAAGGAGGCCTTCGATTACATCAAGCAGAGGAGGAGCATGGTCTCGCCCAA
  CTTTGGCTTCATGGGCCAGCTCCTGCAGTACGAATCTGAGATCCTGCCCTCCACGCCCAA
  CCCCCAGCCTCCCTCCTGCCAAGGGGAGGCAGCAGGCTCTTCACTGATAGGCCATTTGCA
  GACACTGAGCCCTGACATGCAGGGTGCCTACTGCACATTCCCTGCCTCGGTGCTGGCACC
  GGTGCCTACCCACTCAACAGTCTCAGAGCTCAGCAGAAGCCCTGTGGCAACGGCCACATC
  CTGCTAAAACTGGGATGGAGGAATCGGCCCAGCCCCAAGAGCAACTGTGATTTTTGTTTT
  TAAGACTCATGGACATTTCATACCTGTGCAATACTGAAGACCTCATTCTGTCATGCTGCC
  CCAGTGAGATAGTGAGTGGTCACCAGGCTTGCAAATGAACTTCAGACGGACCTCAGGGTA
  GGTTCTCGGGACTGAAGGAAGGCCAAGCCATTACGGGAGCACAGCATGTGCTGACTACTG
  TACTTCCAGACCCCTGCCCTCTTGGGACTGCCCAGTCCTTGCACCTCAGAGTTCGCCTTT
  TCATTTCAAGCATAAGGCAATAAATACCTGCAGCAACGTGGGAGAAAGAAGTTGCTGGAC
  CAGGAGAAAAGGCAGTTATGAAGCCAATTCATTTTGAAGGAAGCACAATTTCCACCTTAT
  TTTTTGAACTTTGGCAGTTTCAATGTCTGTCTCTGTTGCTTCGGGGCATAAGCTGATCAC
  CGTCTAGTTGGGAAAGTAACCCTACAGGGTTTGTAGGGACATGATCAGCATCCTGATTTG
  AACCCTGAAATGTTGTGTAGACACCCTCTTGGGTCCAATGAGGTAGTTGGTTGAAGTAGC
  AAGATGTTGGCTTTTCTGGATTTTTTTTGCCATGGGTTCTTCACTGACCTTGGACTTTGG
  CATGATTCTTAGTCATACTTGAACTTGTCTCATTCCACCTCTTCTCAGAGCAACTCTTCC
  TTTGGGAAAAGAGTTCTTCAGATCATAGACCAAAAAAGTCATACCTTCGAGGTGGTAGCA
  GTAGATTCCAGGAGGAGAAGGGTACTTGCTAGGTATCCTGGGTCAGTGGCGGTGCAAACT
  GGTTTCCTCAGCTGCCTGTCCTTCTGTGTGCTTATGTCTCTTGTGACAATTGTTTTCCTC
  CCTGCCCCTGGAGGTTGTCTTCAAGCTGTGGACTTCTGGGATTTGCAGATTTTGCAACGT
  GGTACTACTTTTTTTTTCTTTTTGTCTGTTAGTTATTTCTCCAGGGGAAAAGGCAATAAT
  TTTCTAAGACCCGTGTGAATGTGAAGAAAAGCAGTATGTTACTGGTTGTTGTTGTTGTTC
  TTGTTTTTTATAGTGTAAAATAAAAATAGTGAAAGGAGAAAAGCAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_004419
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004419.3, NP_004410.3
• RefSeq Size: 2545 bp
• RefSeq ORF: 1155 bp
• Locus ID: 1847
• UniProt ID: Q16690
• Cytogenetics: 10q25.2
• Domains: DSPc, RHOD, PTPc_motif
• Protein Families: Phosphatase
• Protein Pathways: MAPK signaling pathway
• Gene Summary: The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene product inactivates ERK1, is expressed in a variety of tissues with the highest levels in pancreas and brain, and is localized in the nucleus. [provided by RefSeq, Jul 2008]