Endo G (ENDOG) (NM_004435) Human Untagged Clone

CAT#: SC319651

ENDOG (untagged)-Human endonuclease G (ENDOG), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Endo G
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_004435.2 GGGGAAATGCGCAAAGAAAGGACGCCGGGGACACCCGGTTGGGCTCTGCTGCTCCCTTCT
  GGGTTCCGAGGCCCAAGCCCTTGGCAGTGTTTGTGAGTGGAAGGGAGGTCACGCTATCGT
  CCGCGGCCCCAGCAGCCCTGTGCCCTCGTTGGATCCCGCGACGCGGCTCCTTTAAGAGCC
  TCGCGGGTCGCCCGCCGCTAGGTCGCTCCCCGGCCATGCGGGCGCTGCGGGCCGGCCTGA
  CCCTGGCGTTGGGCGCGGGGCTGGGTGCGGTCGTCGAGGGCTGGCGGCGGCGGCGGGAGG
  ACGCGCGGGCGGCGCCGGGACTGCTGGGCCGGCTGCCCGTGCTGCCCGTGGCGGCGGCAG
  CCGAGTTGCCCCCTGTGCCCGGGGGACCCCGCGGCCCGGGCGAGCTGGCCAAGTACGGGC
  TGCCGGGGCTGGCGCAGCTCAAGAGCCGCGAGTCGTACGTGCTGTGCTACGACCCGCGCA
  CCCGCGGCGCGCTCTGGGTGGTGGAGCAGCTGCGACCCGAGCGTCTCCGCGGCGACGGCG
  ACCGGCGCGAGTGCGACTTCCGCGAGGACGACTCGGTGCACGCGTACCACCGTGCCACCA
  ACGCCGACTACCGCGGCAGTGGCTTCGACCGCGGTCACCTGGCCGCCGCCGCCAACCACC
  GCTGGAGCCAGAAGGCCATGGACGACACGTTCTACCTGAGCAACGTCGCGCCCCAGGTGC
  CCCACCTCAACCAGAATGCCTGGAACAACCTGGAGAAATATAGCCGCAGCTTGACCCGCA
  GCTACCAAAACGTCTATGTCTGCACAGGGCCACTCTTCCTGCCCAGGACAGAGGCTGATG
  GGAAATCCTACGTAAAGTACCAGGTCATCGGCAAGAACCACGTGGCAGTGCCCACACACT
  TCTTCAAGGTGCTGATCCTGGAGGCAGCAGGTGGGCAAATTGAGCTCCGCACCTACGTGA
  TGCCCAACGCACCTGTGGATGAGGCCATCCCACTGGAGCGCTTCCTGGTGCCCATCGAGA
  GCATTGAGCGGGCTTCGGGGCTGCTCTTTGTGCCAAACATCCTGGCGCGGGCAGGCAGCC
  TCAAGGCCATCACGGCGGGCAGTAAGTGAGGGTGGAGCCCAGTGAGACTGTGGGTGTGTG
  CAGGCCGGGGAGTATTAAAGGTGGTGATTTTTGGACAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAA
• Restriction Sites: Please inquire
• ACCN: NM_004435
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004435.2, NP_004426.2
• RefSeq Size: 1145 bp
• RefSeq ORF: 894 bp
• Locus ID: 2021
• UniProt ID: Q14249
• Cytogenetics: 9q34.11
• Domains: Endonuclease
• Protein Families: Druggable Genome
• Protein Pathways: Apoptosis
• Gene Summary: The protein encoded by this gene is a nuclear encoded endonuclease that is localized in the mitochondrion. The encoded protein is widely distributed among animals and cleaves DNA at GC tracts. This protein is capable of generating the RNA primers required by DNA polymerase gamma to initiate replication of mitochondrial DNA. [provided by RefSeq, Jul 2008]