KRT6A (NM_005554) Human Untagged Clone

SKU SC319637

KRT6A (untagged)-Human keratin 6A (KRT6A)

Product Data

• Type: Human Untagged Clone
• Target Symbol: KRT6A
• Synonyms: CK-6C; CK-6E; CK6A; CK6C; CK6D; K6A; K6C; K6D; KRT6C; KRT6D; PC3
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_005554.3 CTCTCCTCCAGCCTCTCACACTCTCCTCAGCTCTCTCATCTCCTGGAACCATGGCCAGCA; CATCCACCACCATCAGGAGCCACAGCAGCAGCCGCCGGGGTTTCAGTGCCAGCTCAGCCA; GGCTCCCTGGGGTCAGCCGCTCTGGCTTCAGCAGCGTCTCCGTGTCCCGCTCCAGGGGCA; GTGGTGGCCTGGGTGGTGCATGTGGAGGAGCTGGCTTTGGCAGCCGCAGTCTGTATGGCC; TGGGGGGCTCCAAGAGGATCTCCATTGGAGGGGGCAGCTGTGCCATCAGTGGCGGCTATG; GCAGCAGAGCCGGAGGCAGCTATGGCTTTGGTGGCGCCGGGAGTGGATTTGGTTTCGGTG; GTGGAGCCGGCATTGGCTTTGGTCTGGGTGGTGGAGCCGGCCTTGCTGGTGGCTTTGGGG; GCCCTGGCTTCCCTGTGTGCCCCCCTGGAGGCATCCAAGAGGTCACCGTCAACCAGAGTC; TCCTGACTCCCCTCAACCTGCAAATCGATCCCACCATCCAGCGGGTGCGGGCCGAGGAGC; GTGAACAGATCAAGACCCTCAACAACAAGTTTGCCTCCTTCATCGACAAGGTGCGGTTCC; TGGAGCAGCAGAACAAGGTTCTGGAAACAAAGTGGACCCTGCTGCAGGAGCAGGGCACCA; AGACTGTGAGGCAGAACCTGGAGCCGTTGTTCGAGCAGTACATCAACAACCTCAGGAGGC; AGCTGGACAGCATTGTCGGGGAACGGGGCCGCCTGGACTCAGAGCTCAGAGGCATGCAGG; ACCTGGTGGAGGACTTCAAGAACAAATATGAGGATGAAATCAACAAGCGCACAGCAGCAG; AGAATGAATTTGTGACTCTGAAGAAGGACGTGGATGCTGCCTACATGAACAAGGTTGAAC; TGCAAGCCAAGGCAGACACTCTCACAGACGAGATCAACTTCCTGAGAGCCTTGTATGATG; CAGAGCTGTCCCAGATGCAGACCCACATCTCAGACACATCTGTGGTGCTGTCCATGGACA; ACAACCGCAACCTGGACCTGGACAGCATCATCGCTGAGGTCAAGGCCCAATATGAGGAGA; TTGCTCAGAGAAGCCGGGCTGAGGCTGAGTCCTGGTACCAGACCAAGTACGAGGAGCTGC; AGGTCACAGCAGGCAGACATGGGGACGACCTGCGCAACACCAAGCAGGAGATTGCTGAGA; TCAACCGCATGATCCAGAGGCTGAGATCTGAGATCGACCACGTCAAGAAGCAGTGCGCCA; ACCTGCAGGCCGCCATTGCTGATGCTGAGCAGCGTGGGGAGATGGCCCTCAAGGATGCCA; AGAACAAGCTGGAAGGGCTGGAGGATGCCCTGCAGAAGGCCAAGCAGGACCTGGCCCGGC; TGCTGAAGGAGTACCAGGAGCTGATGAATGTCAAGCTGGCCCTGGACGTGGAGATCGCCA; CCTACCGCAAGCTGCTGGAGGGTGAGGAGTGCAGGCTGAATGGCGAAGGCGTTGGACAAG; TCAACATCTCTGTGGTGCAGTCCACCGTCTCCAGTGGCTATGGCGGTGCCAGTGGTGTCG; GCAGTGGCTTAGGCCTGGGTGGAGGAAGCAGCTACTCCTATGGCAGTGGTCTTGGCGTTG; GAGGTGGCTTCAGTTCCAGCAGTGGCAGAGCCATTGGGGGTGGCCTCAGCTCTGTTGGAG; GCGGCAGTTCCACCATCAAGTACACCACCACCTCCTCCTCCAGCAGGAAGAGCTATAAGC; ACTAAAGTGCGTCTGCTAGCTCTCGGTCCCACAGTCCTCAGGCCCCTCTCTGGCTGCAGA; GCCCTCTCCTCAGGTTGCCTGTCCTCTCCTGGCCTCCAGTCTCCCCTGCTGTCCCAGGTA; GAGCTGGGGATGAATGCTTAGTGCCTTCACTTCTTCTCTCTCTCTCTATACCATCTGAGC; ACCCATTGCTCACCATCAGATCAACCTCTGATTTTACATCATGATGTAATCACCACTGGA; GCTTCACTTTGTTACTAAATTATTAATTTCTTGCCTCCAGTGTTCTATCTCTGAGGCTGA; GCATTATAAGAAAATGACCTCTGCTCCTTTTCATTGCAGAAAATTGCCAGGGGCTTATTT; CAGAACAACTTCCACTTACTTTCCACTGGCTCTCAAACTCTCTAACTTATAAGTGTTGTG; AACCCCCACCCAGGCAGTATCCATGAAAGCACAAGTGACTAGTCCTATGATGTACAAAGC; CTGTATCTCTGTGATGATTTCTGTGCTCTTCGCTCTTTGCAATTGCTAAATAAAGCAGAT; TTATAATACAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA; AAAAA
• Restriction Sites: Please inquire
• ACCN: NM_005554
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_005554.3, NP_005545.1
• RefSeq Size: 2450 bp
• RefSeq ORF: 1695 bp
• Locus ID: 3853
• UniProt ID: P02538
• Cytogenetics: 12q13.13
• Domains: filament
• Summary: The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. As many as six of this type II cytokeratin (KRT6) have been identified; the multiplicity of the genes is attributed to successive gene duplication events. The genes are expressed with family members KRT16 and/or KRT17 in the filiform papillae of the tongue, the stratified epithelial lining of oral mucosa and esophagus, the outer root sheath of hair follicles, and the glandular epithelia. This KRT6 gene in particular encodes the most abundant isoform. Mutations in these genes have been associated with pachyonychia congenita. In addition, peptides from the C-terminal region of the protein have antimicrobial activity against bacterial pathogens. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq, Oct 2014]