KRT6A (NM_005554) Human Untagged Clone

CAT#: SC319637

KRT6A (untagged)-Human keratin 6A (KRT6A)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: KRT6A
• Synonyms: CK-6C; CK-6E; CK6A; CK6C; CK6D; K6A; K6C; K6D; KRT6C; KRT6D; PC3
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_005554.3 CTCTCCTCCAGCCTCTCACACTCTCCTCAGCTCTCTCATCTCCTGGAACCATGGCCAGCA
  CATCCACCACCATCAGGAGCCACAGCAGCAGCCGCCGGGGTTTCAGTGCCAGCTCAGCCA
  GGCTCCCTGGGGTCAGCCGCTCTGGCTTCAGCAGCGTCTCCGTGTCCCGCTCCAGGGGCA
  GTGGTGGCCTGGGTGGTGCATGTGGAGGAGCTGGCTTTGGCAGCCGCAGTCTGTATGGCC
  TGGGGGGCTCCAAGAGGATCTCCATTGGAGGGGGCAGCTGTGCCATCAGTGGCGGCTATG
  GCAGCAGAGCCGGAGGCAGCTATGGCTTTGGTGGCGCCGGGAGTGGATTTGGTTTCGGTG
  GTGGAGCCGGCATTGGCTTTGGTCTGGGTGGTGGAGCCGGCCTTGCTGGTGGCTTTGGGG
  GCCCTGGCTTCCCTGTGTGCCCCCCTGGAGGCATCCAAGAGGTCACCGTCAACCAGAGTC
  TCCTGACTCCCCTCAACCTGCAAATCGATCCCACCATCCAGCGGGTGCGGGCCGAGGAGC
  GTGAACAGATCAAGACCCTCAACAACAAGTTTGCCTCCTTCATCGACAAGGTGCGGTTCC
  TGGAGCAGCAGAACAAGGTTCTGGAAACAAAGTGGACCCTGCTGCAGGAGCAGGGCACCA
  AGACTGTGAGGCAGAACCTGGAGCCGTTGTTCGAGCAGTACATCAACAACCTCAGGAGGC
  AGCTGGACAGCATTGTCGGGGAACGGGGCCGCCTGGACTCAGAGCTCAGAGGCATGCAGG
  ACCTGGTGGAGGACTTCAAGAACAAATATGAGGATGAAATCAACAAGCGCACAGCAGCAG
  AGAATGAATTTGTGACTCTGAAGAAGGACGTGGATGCTGCCTACATGAACAAGGTTGAAC
  TGCAAGCCAAGGCAGACACTCTCACAGACGAGATCAACTTCCTGAGAGCCTTGTATGATG
  CAGAGCTGTCCCAGATGCAGACCCACATCTCAGACACATCTGTGGTGCTGTCCATGGACA
  ACAACCGCAACCTGGACCTGGACAGCATCATCGCTGAGGTCAAGGCCCAATATGAGGAGA
  TTGCTCAGAGAAGCCGGGCTGAGGCTGAGTCCTGGTACCAGACCAAGTACGAGGAGCTGC
  AGGTCACAGCAGGCAGACATGGGGACGACCTGCGCAACACCAAGCAGGAGATTGCTGAGA
  TCAACCGCATGATCCAGAGGCTGAGATCTGAGATCGACCACGTCAAGAAGCAGTGCGCCA
  ACCTGCAGGCCGCCATTGCTGATGCTGAGCAGCGTGGGGAGATGGCCCTCAAGGATGCCA
  AGAACAAGCTGGAAGGGCTGGAGGATGCCCTGCAGAAGGCCAAGCAGGACCTGGCCCGGC
  TGCTGAAGGAGTACCAGGAGCTGATGAATGTCAAGCTGGCCCTGGACGTGGAGATCGCCA
  CCTACCGCAAGCTGCTGGAGGGTGAGGAGTGCAGGCTGAATGGCGAAGGCGTTGGACAAG
  TCAACATCTCTGTGGTGCAGTCCACCGTCTCCAGTGGCTATGGCGGTGCCAGTGGTGTCG
  GCAGTGGCTTAGGCCTGGGTGGAGGAAGCAGCTACTCCTATGGCAGTGGTCTTGGCGTTG
  GAGGTGGCTTCAGTTCCAGCAGTGGCAGAGCCATTGGGGGTGGCCTCAGCTCTGTTGGAG
  GCGGCAGTTCCACCATCAAGTACACCACCACCTCCTCCTCCAGCAGGAAGAGCTATAAGC
  ACTAAAGTGCGTCTGCTAGCTCTCGGTCCCACAGTCCTCAGGCCCCTCTCTGGCTGCAGA
  GCCCTCTCCTCAGGTTGCCTGTCCTCTCCTGGCCTCCAGTCTCCCCTGCTGTCCCAGGTA
  GAGCTGGGGATGAATGCTTAGTGCCTTCACTTCTTCTCTCTCTCTCTATACCATCTGAGC
  ACCCATTGCTCACCATCAGATCAACCTCTGATTTTACATCATGATGTAATCACCACTGGA
  GCTTCACTTTGTTACTAAATTATTAATTTCTTGCCTCCAGTGTTCTATCTCTGAGGCTGA
  GCATTATAAGAAAATGACCTCTGCTCCTTTTCATTGCAGAAAATTGCCAGGGGCTTATTT
  CAGAACAACTTCCACTTACTTTCCACTGGCTCTCAAACTCTCTAACTTATAAGTGTTGTG
  AACCCCCACCCAGGCAGTATCCATGAAAGCACAAGTGACTAGTCCTATGATGTACAAAGC
  CTGTATCTCTGTGATGATTTCTGTGCTCTTCGCTCTTTGCAATTGCTAAATAAAGCAGAT
  TTATAATACAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAA
• Restriction Sites: Please inquire
• ACCN: NM_005554
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005554.3, NP_005545.1
• RefSeq Size: 2450 bp
• RefSeq ORF: 1695 bp
• Locus ID: 3853
• UniProt ID: P02538
• Cytogenetics: 12q13.13
• Domains: filament
• Gene Summary: The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. As many as six of this type II cytokeratin (KRT6) have been identified; the multiplicity of the genes is attributed to successive gene duplication events. The genes are expressed with family members KRT16 and/or KRT17 in the filiform papillae of the tongue, the stratified epithelial lining of oral mucosa and esophagus, the outer root sheath of hair follicles, and the glandular epithelia. This KRT6 gene in particular encodes the most abundant isoform. Mutations in these genes have been associated with pachyonychia congenita. In addition, peptides from the C-terminal region of the protein have antimicrobial activity against bacterial pathogens. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq, Oct 2014]