CCM2 (NM_031443) Human Untagged Clone

CAT#: SC319454

CCM2 (untagged)-Human cerebral cavernous malformation 2 (CCM2), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CCM2
• Synonyms: C7orf22; OSM; PP10187
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_031443.3 CCCGGGTCGAGCATGTAGCGGCTGCTGGCGGCGGGGCTCCCGGGGCGGGCCGGGCGGGCC
  GCGGGAGCCGCACGCGGCGATATGGAAGAGGAGGGCAAGAAGGGCAAGAAGCCTGGAATT
  GTCTCGCCATTTAAACGAGTATTCCTAAAAGGTGAAAAGAGTAGAGATAAGAAAGCCCAT
  GAGAAGGTGACAGAGAGGCGCCCTCTGCACACTGTGGTGTTGTCATTGCCTGAGCGCGTC
  GAGCCAGACAGACTGCTGAGCGACTATATTGAGAAGGAGGTAAAGTATTTAGGTCAGTTA
  ACGTCCATACCAGGATACCTGAATCCCTCCAGTAGGACTGAAATCCTGCATTTCATAGAC
  AATGCAAAGAGAGCCCACCAGCTTCCGGGACACTTGACTCAGGAGCACGATGCTGTGCTC
  AGCCTGTCTGCGTACAACGTCAAGCTGGCCTGGAGGGACGGGGAGGATATCATCCTCAGG
  GTGCCCATCCATGACATCGCCGCCGTCTCCTATGTTCGGGATGACGCTGCACACCTGGTG
  GTCCTGAAGACAGCCCAGGACCCAGGGATCTCCCCCAGCCAGAGTCTGTGTGCGGAAAGT
  TCCAGAGGCCTCAGTGCAGGCTCCCTGTCGGAGAGTGCAGTTGGGCCCGTGGAGGCATGC
  TGCCTGGTCATCCTGGCTGCAGAGAGCAAGGTCGCTGCGGAGGAGCTTTGCTGTCTGCTA
  GGCCAGGTCTTCCAGGTTGTTTACACGGAGTCCACCATCGACTTTCTGGACAGAGCGATA
  TTTGATGGGGCCTCTACCCCGACCCACCACCTGTCCCTGCACAGCGATGACTCTTCTACA
  AAAGTGGACATTAAGGAGACCTACGAGGTGGAAGCCAGCACTTTCTGCTTCCCTGAATCT
  GTGGATGTGGGTGGTGCATCACCCCACAGCAAGACCATCAGTGAGAGCGAGCTGAGCGCC
  AGCGCCACTGAGCTGCTGCAGGACTACATGCTGACGCTGCGCACCAAGCTGTCATCACAG
  GAGATCCAGCAGTTTGCAGCACTGCTGCACGAGTACCGCAATGGGGCCTCTATCCACGAG
  TTCTGCATCAACCTGCGGCAGCTCTACGGGGACAGCCGCAAGTTCCTGCTGCTTGGTCTG
  AGGCCCTTCATCCCTGAGAAGGACAGCCAGCACTTCGAGAACTTCCTGGAGACCATTGGC
  GTGAAGGATGGCCGCGGCATCATCACTGACAGCTTTGGCAGGCACCGGCGGGCCCTGAGC
  ACCACATCCAGTTCCACCACCAATGGGAACAGGGCCACGGGCAGCTCTGATGACCGGTCG
  GCACCCTCAGAGGGGGATGAGTGGGACCGCATGATCTCGGACATCAGCAGCGACATTGAG
  GCGCTGGGCTGCAGCATGGACCAGGACTCAGCATGATGGACAGTGGATGGGGGGGCACCC
  ACACCTTCCGCGCAGTCGTCATAGGCCTTCCCAGAAGGAGCTGCCCAGACCTGCGTGTCA
  GCCCTTGGTGGTGGCCAGGGAGAGGCGCCCGGTGCAGATGGCCCCGGGCGGCCCAGGTCC
  TCTACTGTGAAGGAGCAGGGAGCTGCCGAGGGACACGAGCCTCAGTGCGGGGTGGAAGGC
  TCTTTGCCTTGTCCACCAGGGCTCAGCCAAGCCCTGCAGTGTGTCCCCGCTCGGGGAGGG
  CCCGGCCGAGCGGGCAGGGAGAGCCAGTCCTGTCGGCTGGGCCCTTGGACGGCTGTCAGT
  TTTGCACATGATGTTCCTATTGTAACTCTCAGAGACCTTAAAAAGAAGTTTACTGCAATG
  TGAATAATTTAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_031443
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_031443.3, NP_113631.1
• RefSeq Size: 1904 bp
• RefSeq ORF: 1335 bp
• Locus ID: 83605
• UniProt ID: Q9BSQ5
• Cytogenetics: 7p13
• Gene Summary: This gene encodes a scaffold protein that functions in the stress-activated p38 Mitogen-activated protein kinase (MAPK) signaling cascade. The protein interacts with SMAD specific E3 ubiquitin protein ligase 1 (also known as SMURF1) via a phosphotyrosine binding domain to promote RhoA degradation. The protein is required for normal cytoskeletal structure, cell-cell interactions, and lumen formation in endothelial cells. Mutations in this gene result in cerebral cavernous malformations. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Nov 2009]
  Transcript Variant: This variant (2) represents use of an alternate promoter and 5' UTR and uses a distinct start codon, compared to variant 1. The resulting isoform (2) has a shorter and distinct N-terminus, compared to isoform 1.