KLF5 (NM_001730) Human Untagged Clone

CAT#: SC319166

KLF5 (untagged)-Human Kruppel-like factor 5 (intestinal) (KLF5)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: KLF5
• Synonyms: BTEB2; CKLF; IKLF
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001730.3 CGAGGTACGTGCGCTCGCGGTTCTCTCGCGGAGGTCGGCGGTGGCGGGAGCGGGCTCCGG
  AGAGCCTGAGAGCACGGTGGGGCGGGGCGGGAGAAAGTGGCCGCCCGGAGGACGTTGGCG
  TTTACGTGTGGAAGAGCGGAAGAGTTTTGCTTTTCGTGCGCGCCTTCGAAAACTGCCTGC
  CGCTGTCTGAGGAGTCCACCCGAAACCTCCCCTCCTCCGCCGGCAGCCCCGCGCTGAGCT
  CGCCGACCCAAGCCAGCGTGGGCGAGGTGGGAAGTGCGCCCGACCCGCGCCTGGAGCTGC
  GCCCCCGAGTGCCCATGGCTACAAGGGTGCTGAGCATGAGCGCCCGCCTGGGACCCGTGC
  CCCAGCCGCCGGCGCCGCAGGACGAGCCGGTGTTCGCGCAGCTCAAGCCGGTGCTGGGCG
  CCGCGAATCCGGCCCGCGACGCGGCGCTCTTCCCCGGCGAGGAGCTGAAGCACGCGCACC
  ACCGCCCGCAGGCGCAGCCCGCGCCCGCGCAGGCCCCGCAGCCGGCCCAGCCGCCCGCCA
  CCGGCCCGCGGCTGCCTCCAGAGGACCTGGTCCAGACAAGATGTGAAATGGAGAAGTATC
  TGACACCTCAGCTTCCTCCAGTTCCTATAATTCCAGAGCATAAAAAGTATAGACGAGACA
  GTGCCTCAGTCGTAGACCAGTTCTTCACTGACACTGAAGGGTTACCTTACAGTATCAACA
  TGAACGTCTTCCTCCCTGACATCACTCACCTGAGAACTGGCCTCTACAAATCCCAGAGAC
  CGTGCGTAACACACATCAAGACAGAACCTGTTGCCATTTTCAGCCACCAGAGTGAAACGA
  CTGCCCCTCCTCCGGCCCCGACCCAGGCCCTCCCTGAGTTCACCAGTATATTCAGCTCAC
  ACCAGACCGCAGCTCCAGAGGTGAACAATATTTTCATCAAACAAGAACTTCCTACACCAG
  ATCTTCATCTTTCTGTCCCTACCCAGCAGGGCCACCTGTACCAGCTACTGAATACACCGG
  ATCTAGATATGCCCAGTTCTACAAATCAGACAGCAGCAATGGACACTCTTAATGTTTCTA
  TGTCAGCTGCCATGGCAGGCCTTAACACACACACCTCTGCTGTTCCGCAGACTGCAGTGA
  AACAATTCCAGGGCATGCCCCCTTGCACATACACAATGCCAAGTCAGTTTCTTCCACAAC
  AGGCCACTTACTTTCCCCCGTCACCACCAAGCTCAGAGCCTGGAAGTCCAGATAGACAAG
  CAGAGATGCTCCAGAATTTAACCCCACCTCCATCCTATGCTGCTACAATTGCTTCTAAAC
  TGGCAATTCACAATCCAAATTTACCCACCACCCTGCCAGTTAACTCACAAAACATCCAAC
  CTGTCAGATACAATAGAAGGAGTAACCCCGATTTGGAGAAACGACGCATCCACTACTGCG
  ATTACCCTGGTTGCACAAAAGTTTATACCAAGTCTTCTCATTTAAAAGCTCACCTGAGGA
  CTCACACTGGTGAAAAGCCATACAAGTGTACCTGGGAAGGCTGCGACTGGAGGTTCGCGC
  GATCGGATGAGCTGACCCGCCACTACCGGAAGCACACAGGCGCCAAGCCCTTCCAGTGCG
  GGGTGTGCAACCGCAGCTTCTCGCGCTCTGACCACCTGGCCCTGCATATGAAGAGGCACC
  AGAACTGAGCACTGCCCGTGTGACCCGTTCCAGGTCCCCTGGGCTCCCTCAAATGACAGA
  CCTAA
• Restriction Sites: Please inquire
• ACCN: NM_001730
• Insert Size: 1700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001730.3, NP_001721.2
• RefSeq Size: 3350 bp
• RefSeq ORF: 1374 bp
• Locus ID: 688
• UniProt ID: Q13887
• Cytogenetics: 13q22.1
• Domains: zf-C2H2
• Protein Families: Embryonic stem cells, ES Cell Differentiation/IPS, Induced pluripotent stem cells, Transcription Factors
• Gene Summary: This gene encodes a member of the Kruppel-like factor subfamily of zinc finger proteins. The encoded protein is a transcriptional activator that binds directly to a specific recognition motif in the promoters of target genes. This protein acts downstream of multiple different signaling pathways and is regulated by post-translational modification. It may participate in both promoting and suppressing cell proliferation. Expression of this gene may be changed in a variety of different cancers and in cardiovascular disease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2013]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).