alpha Adaptin (AP2A2) (NM_012305) Human Untagged Clone

CAT#: SC319157

AP2A2 (untagged)-Human adaptor-related protein complex 2, alpha 2 subunit (AP2A2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: alpha Adaptin
• Synonyms: ADTAB; CLAPA2; HIP-9; HIP9; HYPJ
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_012305.2 CTGGGACCCTGAGGCGGCCGTGGTTAGGCGGCTCCCCGGCGGCTCCTCCGCGGCGGTGAC
  GGCGACCGCACTCCCCGCTTCCCGCTCCCCGCGCTCCTCCGCCCGGGTCCGCCAGCCGAG
  GCCGCTCCCGAGCGTCGGAAGATGCCGGCCGTGTCCAAGGGGGACGGGATGCGGGGCCTG
  GCGGTCTTCATCTCGGATATCCGCAACTGTAAAAGTAAAGAAGCAGAAATAAAAAGGATA
  AACAAGGAACTGGCAAATATCAGATCAAAATTTAAAGGTGACAAGGCTCTTGATGGCTAT
  AGTAAAAAAAAGTACGTCTGCAAGTTGCTCTTCATCTTTCTCCTTGGTCATGACATTGAC
  TTTGGACACATGGAGGCTGTGAACCTGCTGAGTTCAAACAGATACACGGAAAAGCAGATC
  GGCTACCTTTTCATCTCTGTGTTGGTGAACTCAAACAGTGAGCTGATCCGCCTGATCAAC
  AACGCCATCAAGAATGACCTGGCCAGCCGCAACCCCACCTTCATGGGCCTGGCCCTGCAC
  TGCATCGCCAGCGTGGGCAGCCGGGAGATGGCCGAGGCCTTCGCCGGGGAGATCCCTAAG
  GTCCTCGTAGCCGGAGACACTATGGACAGCGTGAAGCAGAGCGCGGCCCTGTGCTTGCTG
  CGCCTGTACAGGACGTCCCCCGATCTTGTCCCCATGGGCGACTGGACATCCCGAGTGGTG
  CACCTGCTCAATGACCAGCACTTGGGTGTGGTAACTGCAGCCACAAGTCTGATCACCACT
  TTAGCACAGAAGAACCCAGAAGAGTTTAAAACCTCCGTGTCTCTGGCTGTCTCTAGGCTA
  AGCAGAATCGTGACGTCTGCATCCACAGATCTCCAGGATTACACTTACTATTTTGTCCCG
  GCTCCCTGGCTGTCTGTCAAACTGCTGAGACTGCTGCAGTGCTACCCACCCCCAGACCCT
  GCAGTGCGAGGCCGCCTGACTGAGTGCCTGGAGACCATCCTGAACAAAGCCCAAGAACCG
  CCCAAGTCGAAGAAGGTCCAGCACTCCAACGCGAAGAATGCCGTGCTCTTCGAGGCCATC
  AGCTTAATCATTCACCATGACAGTGAGCCGAACCTGCTCGTCCGTGCCTGCAACCAGTTG
  GGCCAGTTTCTGCAGCACCGCGAGACCAACCTGCGCTACCTGGCCCTGGAGAGCATGTGC
  ACGCTGGCCAGCTCTGAGTTCTCCCATGAGGCTGTCAAGACGCACATCGAGACGGTCATC
  AACGCCCTGAAGACTGAGCGGGACGTGAGCGTGCGGCAGCGGGCCGTGGACCTCCTCTAC
  GCCATGTGCGACCGCAGCAACGCCCCACAGATCGTGGCCGAGATGCTGAGCTATCTGGAG
  ACAGCTGACTACTCCATCCGAGAAGAGATTGTGCTGAAGGTCGCCATCCTGGCTGAGAAG
  TACGCGGTGGACTACACCTGGTATGTGGATACCATCTTGAACTTGATCCGAATTGCTGGT
  GATTACGTGAGTGAAGAGGTGTGGTACCGAGTCATTCAGATCGTCATCAACCGGGACGAC
  GTGCAGGGCTACGCGGCCAAGACTGTGTTCGAGGCTCTTCAGGCTCCCGCGTGCCACGAG
  AACCTGGTCAAAGTGGGCGGCTACATCCTGGGGGAGTTTGGAAACTTGATAGCTGGAGAC
  CCGAGATCCAGCCCGCTGATCCAGTTCCACCTGCTGCACTCCAAGTTCCACCTGTGCAGC
  GTCCCCACCCGCGCGCTGCTCCTGTCCACCTACATCAAGTTCGTGAACCTCTTCCCGGAG
  GTGAAGCCCACCATCCAGGACGTGCTGCGCAGCGACAGCCAGCTCAGGAACGCAGACGTG
  GAGCTGCAGCAGCGTGCTGTGGAGTACCTGCGGCTCAGCACCGTGGCCAGCACCGACATT
  CTGGCGACCGTGCTGGAGGAGATGCCCCCATTCCCGGAGCGGGAGTCCTCCATCTTGGCA
  AAGCTCAAGAAGAAGAAGGGCCCCAGCACGGTGACAGACCTGGAGGACACCAAGCGGGAC
  AGGAGTGTGGACGTGAACGGGGGTCCTGAGCCTGCCCCAGCCAGTACCAGCGCCGTGTCT
  ACGCCTTCTCCGTCGGCAGACCTGCTGGGTCTCGGGGCTGCCCCCCCTGCCCCCGCGGGC
  CCCCCACCCTCCTCCGGCGGCAGCGGGCTGCTCGTGGACGTGTTCTCAGACTCGGCCTCT
  GTGGTCGCGCCTCTCGCTCCTGGCTCCGAAGACAACTTTGCCAGGTTTGTTTGTAAAAAC
  AATGGTGTGTTGTTTGAAAACCAGCTGCTTCAAATTGGACTTAAGTCTGAATTTCGGCAG
  AATTTAGGTCGGATGTTTATCTTTTATGGTAATAAGACCTCCACGCAGTTCCTAAACTTT
  ACCCCAACACTAATCTGTTCAGACGACCTTCAGCCTAACCTGAACCTGCAGACCAAGCCC
  GTGGACCCGACCGTGGAGGGGGGCGCGCAGGTGCAGCAGGTGGTCAACATAGAGTGCGTG
  TCCGACTTCACGGAGGCGCCAGTCCTCAACATTCAGTTCAGGTATGGGGGCACCTTCCAG
  AACGTGTCTGTGCAGCTGCCCATCACTCTCAACAAATTCTTCCAGCCGACAGAAATGGCT
  TCTCAGGATTTCTTTCAACGTTGGAAGCAGTTGAGCAATCCACAGCAGGAAGTGCAGAAC
  ATCTTCAAAGCAAAGCACCCAATGGACACAGAAGTCACCAAAGCCAAGATCATTGGATTT
  GGTTCTGCACTTCTTGAAGAAGTTGATCCTAATCCTGCGAATTTCGTGGGAGCTGGAATC
  ATCCACACGAAAACCACCCAGATTGGATGCCTGCTGCGCTTGGAGCCGAACCTGCAAGCC
  CAGATGTACCGGCTCACGCTGCGCACAAGTAAGGAAGCCGTTTCTCAGAGATTATGTGAA
  TTGCTCTCAGCGCAGTTTTAGTCCTGAGGATGGAAGACCAGGCTTGTGTGTCTTGTGTTG
  TCTTCGTCTGTGCCGTTTGTCTTCGTGGCCATCCTGCAGATGAGCACCGTGTCCAGTGCC
  ACAGCACAAGGCGCCTCCCCGCCCCGCCGCCCCACACCTCTCCCCTTTGGGCTGGACGGG
  AACACACGTGTGTGGCTCAGGAGGAAAAGCTCAGCCTGGACTGTGGCAGCCACGGCAGAA
  GGTGGATCTTGGGATCAATTTTTATAAAAATCGAGACAGTTCTGTGGTTAAATCTACAAA
  TTAAAGGGAAATTAGAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_012305
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012305.2, NP_036437.1
• RefSeq Size: 4616 bp
• RefSeq ORF: 2820 bp
• Locus ID: 161
• UniProt ID: O94973
• Cytogenetics: 11p15.5
• Protein Pathways: Endocytosis, Huntington's disease
• Gene Summary: The protein encoded by this gene is a subunit of the AP-2 adaptor protein complex, which is involved in linking lipid and protein membrane components with the clathrin lattice. This interaction supports the formation of clathrin-coated vesicles, and the encoded subunit aids in the process by binding polyphosphoinositide-containing lipids in the cell membrane. [provided by RefSeq, Nov 2016]
  Transcript Variant: This variant (2) uses an alternate in-frame splice site in the central coding region, compared to variant 1, resulting in an isoform (2) that is 1 aa shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.