DDX6 (NM_004397) Human Untagged Clone

CAT#: SC317807

DDX6 (untagged)-Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 6 (DDX6)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DDX6
• Synonyms: HLR2; IDDILF; P54; RCK; Rck/p54
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC317807 representing NM_004397.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGAGCACGGCCAGAACAGAGAACCCTGTTATAATGGGTCTGTCCAGTCAAAATGGTCAGCTGAGAGGC
  CCTGTGAAACCCACTGGTGGCCCTGGAGGAGGGGGCACACAGACACAGCAACAGATGAACCAGCTGAAA
  AACACCAACACAATCAATAATGGCACTCAGCAGCAAGCACAGAGTATGACCACCACTATTAAACCTGGT
  GATGACTGGAAAAAGACTTTAAAACTCCCTCCAAAGGATCTAAGAATCAAAACTTCGGATGTGACCTCC
  ACAAAAGGAAATGAGTTTGAAGATTACTGTTTGAAACGGGAGTTACTGATGGGAATTTTTGAAATGGGC
  TGGGAAAAGCCATCTCCTATTCAGGAGGAGAGCATTCCCATTGCTTTATCTGGTAGGGATATCTTAGCT
  AGAGCAAAAAATGGAACAGGCAAGAGCGGTGCCTACCTCATTCCCTTACTTGAACGGCTAGACCTGAAG
  AAGGACAATATACAAGCAATGGTGATTGTTCCCACTAGAGAACTTGCTCTACAGGTCAGTCAAATTTGC
  ATCCAGGTCAGCAAACACATGGGAGGGGCCAAAGTGATGGCAACCACAGGAGGAACCAATTTACGAGAT
  GACATAATGAGGCTTGATGATACAGTGCACGTGGTGATTGCTACCCCTGGGAGAATCCTGGATCTTATT
  AAGAAAGGAGTAGCAAAGGTTGATCATGTCCAGATGATAGTATTGGATGAGGCAGATAAGTTGCTGTCA
  CAGGATTTTGTGCAGATAATGGAGGATATTATTCTCACGCTACCTAAAAACAGGCAGATTTTACTATAT
  TCCGCTACTTTCCCTCTTAGTGTACAGAAGTTCATGAATTCCCATTTGCAGAAACCCTATGAGATTAAC
  CTGATGGAGGAACTAACTCTGAAGGGAGTAACCCAGTACTACGCATATGTAACTGAGCGCCAAAAAGTA
  CACTGCCTCAACACACTTTTCTCCAGGCTTCAGATAAACCAGTCGATCATTTTCTGTAACTCCTCTCAG
  CGAGTTGAATTGCTAGCCAAGAAGATTTCTCAACTGGGTTATTCTTGCTTCTATATTCATGCTAAAATG
  AGGCAGGAACATCGAAATCGTGTATTTCATGATTTCCGAAATGGCTTATGCCGCAATCTTGTTTGCACT
  GATCTGTTTACCCGAGGTATTGATATACAAGCTGTGAATGTGGTAATAAACTTTGATTTCCCAAAGCTG
  GCAGAGACCTATCTCCATCGTATTGGAAGATCAGGTCGCTTTGGTCATCTTGGCTTAGCCATCAACTTG
  ATCACATATGATGATCGCTTCAACCTGAAAAGTATTGAGGAGCAGCTGGGAACAGAAATTAAACCTATT
  CCGAGCAACATTGATAAGAGCCTGTATGTGGCAGAATACCACAGCGAGCCTGTAGAAGATGAGAAACCT
  TAA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_004397
• Insert Size: 1452 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004397.5
• RefSeq Size: 6246 bp
• RefSeq ORF: 1452 bp
• Locus ID: 1656
• UniProt ID: P26196
• Cytogenetics: 11q23.3
• Domains: DEAD, helicase_C
• Protein Pathways: RNA degradation
• MW: 54.4 kDa
• Gene Summary: This gene encodes a member of the DEAD box protein family. The protein is an RNA helicase found in P-bodies and stress granules, and functions in translation suppression and mRNA degradation. It is required for microRNA-induced gene silencing. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Mar 2012]
  Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein.