SPCS2 (NM_014752) Human Untagged Clone

CAT#: SC317328

SPCS2 (untagged)-Human signal peptidase complex subunit 2 homolog (S. cerevisiae) (SPCS2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SPCS2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC317328 representing NM_014752.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGCGGCGGCAGCTGTACAGGGCGGGAGAAGCGGTGGTAGCGGAGGCTGTAGTGGGGCTGGTGGTGCT
  TCCAACTGCGGGACAGGAAGTGGCCGTAGCGGCTTGTTGGATAAGTGGAAGATAGATGATAAGCCTGTA
  AAAATTGACAAGTGGGATGGATCAGCTGTGAAAAACTCTTTGGATGATTCTGCCAAAAAGGTACTTCTG
  GAAAAATACAAATATGTGGAGAATTTTGGTCTAATTGATGGTCGCCTCACCATCTGTACAATCTCCTGT
  TTCTTTGCCATAGTGGCTTTGATTTGGGATTATATGCACCCCTTTCCAGAGTCCAAACCCGTTTTGGCT
  TTGTGTGTCATATCCTATTTTGTGATGATGGGGATTCTGACCATTTATACCTCATATAAGGAGAAGAGC
  ATCTTTCTCGTGGCCCACAGGAAAGATCCTACAGGAATGGATCCTGATGATATTTGGCAGCTGTCCTCC
  AGTCTTAAAAGGTTTGATGACAAATACACCTTGAAGCTGACCTTCATCAGTGGGAGAACAAAGCAGCAG
  CGGGAAGCCGAGTTCACAAAGTCCATTGCTAAGTTTTTTGACCACAGTGGGACACTGGTCATGGATGCA
  TATGAGCCTGAAATATCCAGGCTCCATGACAGTCTTGCCATAGAAAGAAAAATAAAGTAG
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_014752
• Insert Size: 681 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014752.2
• RefSeq Size: 2731 bp
• RefSeq ORF: 681 bp
• Locus ID: 9789
• UniProt ID: Q15005
• Cytogenetics: 11q13.4
• Protein Families: Protease, Transmembrane
• MW: 25 kDa
• Gene Summary: Component of the microsomal signal peptidase complex which removes signal peptides from nascent proteins as they are translocated into the lumen of the endoplasmic reticulum.[UniProtKB/Swiss-Prot Function]