SIGLEC14 (NM_001098612) Human Untagged Clone

CAT#: SC316370

SIGLEC14 (untagged)-Human sialic acid binding Ig-like lectin 14 (SIGLEC14)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SIGLEC14
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC316370 representing NM_001098612.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGCTGCCCCTGCTGCTGCTGCCCCTGCTGTGGGGGGGGTCCCTGCAGGAGAAGCCAGTGTACGAGCTG
  CAAGTGCAGAAGTCGGTGACGGTGCAGGAGGGCCTGTGCGTCCTTGTGCCCTGCTCCTTCTCTTACCCC
  TGGAGATCCTGGTATTCCTCTCCCCCACTCTACGTCTACTGGTTCCGGGACGGGGAGATCCCATACTAC
  GCTGAGGTTGTGGCCACAAACAACCCAGACAGAAGAGTGAAGCCAGAGACCCAGGGCCGATTCCGCCTC
  CTTGGGGATGTCCAGAAGAAGAACTGCTCCCTGAGCATCGGAGATGCCAGAATGGAGGACACGGGAAGC
  TATTTCTTCCGCGTGGAGAGAGGAAGGGATGTAAAATATAGCTACCAACAGAATAAGCTGAACTTGGAG
  GTGACAGCCCTGATAGAGAAACCCGACATCCACTTTCTGGAGCCTCTGGAGTCCGGCCGCCCCACAAGG
  CTGAGCTGCAGCCTTCCAGGATCCTGTGAAGCGGGACCACCTCTCACATTCTCCTGGACGGGGAATGCC
  CTCAGCCCCCTGGACCCCGAGACCACCCGCTCCTCGGAGCTCACCCTCACCCCCAGGCCCGAGGACCAT
  GGCACCAACCTCACCTGTCAGGTGAAACGCCAAGGAGCTCAGGTGACCACGGAGAGAACTGTCCAGCTC
  AATGTCTCCTATGCTCCACAGAACCTCGCCATCAGCATCTTCTTCAGAAATGGCACAGGCACAGCCCTG
  CGGATCCTGAGCAATGGCATGTCGGTGCCCATCCAGGAGGGCCAGTCCCTGTTCCTCGCCTGCACAGTT
  GACAGCAACCCCCCTGCCTCACTGAGCTGGTTCCGGGAGGGAAAAGCCCTCAATCCTTCCCAGACCTCA
  ATGTCTGGGACCCTGGAGCTGCCTAACATAGGAGCTAGAGAGGGAGGGGAATTCACCTGCCGGGTTCAG
  CATCCGCTGGGCTCCCAGCACCTGTCCTTCATCCTTTCTGTGCAGAGAAGCTCCTCTTCCTGCATATGT
  GTAACTGAGAAACAGCAGGGCTCCTGGCCCCTCGTCCTCACCCTGATCAGGGGGGCTCTCATGGGGGCT
  GGCTTCCTCCTCACCTATGGCCTCACCTGGATCTACTATACCAGGTGTGGAGGCCCCCAGCAGAGCAGG
  GCTGAGAGGCCTGGCTGA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001098612
• Insert Size: 1191 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001098612.1
• RefSeq Size: 2113 bp
• RefSeq ORF: 1191 bp
• Locus ID: 100049587
• UniProt ID: Q08ET2
• Cytogenetics: 19q13.41
• MW: 44 kDa
• Gene Summary: Putative adhesion molecule. Sialic acid-binding paired receptor which may activate associated receptors.[UniProtKB/Swiss-Prot Function]