SSB (NM_003142) Human Untagged Clone

CAT#: SC316315

SSB (untagged)-Human Sjogren syndrome antigen B (autoantigen La) (SSB)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SSB
• Synonyms: La; La/SSB; LARP3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_003142 edited
  CGTTGCTGTTGCTGTTTGTGAGCCTGTGGCGCGGCTTCTGTGGGCCGGAACCTTAAAGAT
  AGCCGCAATGGCTGAAAATGGTGATAATGAAAAGATGGCTGCCCTGGAGGCCAAAATCTG
  TCATCAAATTGAGTATTATTTTGGCGACTTCAATTTGCCACGGGACAAGTTTCTAAAGGA
  ACAGATAAAACTGGATGAAGGCTGGGTACCTTTGGAGATAATGATAAAATTCAACAGGTT
  GAACCGTCTAACAACAGACTTTAATGTAATTGTGGAAGCATTGAGCAAATCCAAGGCAGA
  ACTCATGGAAATCAGTGAAGATAAAACTAAAATCAGAAGGTCTCCAAGCAAACCCCTACC
  TGAAGTGACTGATGAGTATAAAAATGATGTAAAAAACAGATCTGTTTATATTAAAGGCTT
  CCCAACTGATGCAACTCTTGATGACATAAAAGAATGGTTAGAAGATAAAGGTCAAGTACT
  AAATATTCAGATGAGAAGAACATTGCATAAAGCATTTAAGGGATCAATTTTTGTTGTGTT
  TGATAGCATTGAATCTGCTAAGAAATTTGTAGAGACCCCTGGCCAGAAGTACAAAGAAAC
  AGACCTGCTAATACTTTTCAAGGACGATTACTTTGCCAAAAAAAATGAAGAAAGAAAACA
  AAATAAAGTGGAAGCTAAATTAAGAGCTAAACAGGAGCAAGAAGCAAAACAAAAGTTAGA
  AGAAGATGCTGAAATGAAATCTCTAGAAGAAAAGATTGGATGCTTGCTGAAATTTTCGGG
  TGATTTAGATGATCAGACCTGTAGAGAAGATTTACACATACTTTTCTCAAATCATGGTGA
  AATAAAATGGATAGACTTCGTCAGAGGAGCAAAAGAGGGGATAATTCTATTTAAAGAAAA
  AGCCAAGGAAGCATTGGGTAAAGCCAAAGATGCAAATAATGGTAACCTACAATTAAGGAA
  CAAAGAAGTGACTTGGGAAGTACTAGAAGGAGAGGTGGAAAAAGAAGCACTGAAGAAAAT
  AATAGAAGACCAACAAGAATCCCTAAACAAATGGAAGTCAAAAGGTCGTAGATTTAAAGG
  AAAAGGAAAGGGTAATAAAGCTGCCCAGCCTGGGTCTGGTAAAGGAAAAGTACAGTTTCA
  GGGCAAGAAAACGAAATTTGCTAGTGATGATGAACATGATGAACATGATGAAAATGGTGC
  AACTGGACCTGTGAAAAGAGCAAGAGAAGAAACAGACAAAGAAGAACCTGCATCCAAACA
  ACAGAAAACAGAAAATGGTGCTGGAGACCAGTAGTTTAGTAAACCAATYTTTTATTCATT
  TTAAATAGGTTTTAAACGACTTTTGTTTGCGGGGCTTTTAAAAGGAAAACCGAATTAGGT
  CCACTTCAATGTCCACCTGTGAGAAAGGAAAAATTTTTTTGTTGTTTAACTTGTCTTTTT
  GTTATGCAAATGAGATTTCTTTGAATGTATTGTTCTGTTTGTGTTATTTCAGATGATTCA
  AATATCAAAAGGAAGATTCTTCCATTAAATTGCCTTTGTAATATGAGAATGTATTAGTAC
  AAACTAACTAATAAAATATATACTATATGAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003142 unedited
  TAATACGACTCACTATAGGGCGGCCGCGACATTCGCACGAGGCGTTGCTGTTGCTGTTTG
  TGAGCCTGTGGCGCGGCTTCTGTGGGCCGGAACCTTAAAGATAGCCGCAATGGCTGAAAA
  TGGTGATAATGAAAAGATGGCTGCCCTGGAGGCCAAAATCTGTCATCAAATTGAGTATTA
  TTTTGGCGACTTCAATTTGCCACGGGACAAGTTTCTAAAGGAACAGATAAAACTGGATGA
  AGGCTGGGTACCTTTGGAGATAATGATAAAATTCAACAGGTTGAACCGTCTAACAACAGA
  CTTTAATGTAATTGTGGAAGCATTGAGCAAATCCAAGGCAGAACTCATGGAAATCAGTGA
  AGATAAAACTAAAATCAGAAGGTCTCCAAGCAAACCCCTACCTGAAGTGACTGATGAGTA
  TAAAAATGATGTAAAAAACAGATCTGTTTATATTAAAGGCTTCCCAACTGATGCAACTCT
  TGATGACATAAAAGAATGGTTAGAAGATAAAGGTCAAGTACTAAATATTCAGATGAGAAG
  AACATTGCATAAAGCATTTAAGGGATCAATTTTTGTTGTGTTTGATAGCATTGAATCTGC
  TAAGAAATTTGTAGAGACCCCTGGCCAGAAGTACAAAGAAACAGACCTGCTAATACTTTT
  CAAGGACGATTACTTTGCCAAAAAAAATGAAGAAAGAAAACAAAATAAAGTGGAAGCTAA
  ATTAAGAGCTAAACAGGAGCAAGAAGCAAAACAAAGTTAGAAGAGATGCTGAAATGAAAT
  CTCTAGAAGAAAGATNGACTGCTGCTGAAATTTCGGTGATTTAGAGATCAGACTGTAGAG
  AAGATTACACATACTTTNTNAAATATGNTGAATAAATGGATGACCTTGTCAGAGAGCAAG
  AGGGGATAATTCATTTAAAGAAAAGCAAGNNAGCTTGGGTAAGCCAAGATGCAATATGGT
  ACCTCANTAA
  
  3' Read Nucleotide Sequence

  >Forward primer walk for NM_003142 unedited
  AACATGGTTAGAGAAAAGGTCAGTACTAAATACCTCAGATGAGAAGAACATTGCATAAAG
  CATTTAAGGGATCAATTTTTGTTGTGTTTGATAGCATTGAATCTGCTAAGAAATTTGTAG
  AGACCCCTGGCCAGAAGTACAAAGAAACAGACCTGCTAATACTTTTCAAGGACGATTACT
  TTGCCAAAAAAAATGAAGAAAGAAAACAAAATAAAGTGGAAGCTAAATTAAGAGCTAAAC
  AGGAGCAAGAAGCAAAACAAAAGTTAGAAGAAGATGCTGAAATGAAATCTCTAGAAGAAA
  AGATTGGATGCTTGCTGAAATTTTCGGGTGATTTAGATGATCAGACCTGTAGAGAAGATT
  TACACATACTTTTCTCAAATCATGGTGAAATAAAATGGATAGACTTCGTCAGAGGAGCAA
  AAGAGGGGATAATTCTATTTAAAGAAAAAGCCAAGGAAGCATTGGGTAAAGCCAAAGATG
  CAAATAATGGTAACCTACAATTAAGGAACAAAGAAGTGACTTGGGAAGTACTAGAAGGAG
  AGGTGGAAAAAGAAGCACTGAAGAAAATAATAGAAGACCAACAAGAATCCCTAAACAAAT
  GGAAGTCAAAAGGTCGTAGATTTAAAGGAAAAGGAAAGGGTAATAAAGCTGCCCAGCCTG
  GGTCTGGTAAAGGAAAAGTACAGTTTCAGGGCAAGAAAACGAAATTTGCTAGTGATGATG
  AACATGATGAACATGATGAAAATGGTGCAACTGGACCTGTGAAAAGAGCAAGAGAGAACA
  GACAAAGAAGACCCTGCATCCAAACAACAGAAAACAGAAATGGTGCTGAGACAGTAGTTT
  AGTAAACCAATTTTTATTCATTTTAAATAGTTTTAAACGACTTTTGTTGCGGGCTTTAAG
  AAACGATAGTCACTCATGTCCACCTGTGAGAAAGAAATTTTTTTGTTGTTTAACTTGTTC
  TTTTTGTTATGCA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003142
• Insert Size: 1600 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.NA
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003142.3, NP_003133.1
• RefSeq Size: 1673 bp
• RefSeq ORF: 1227 bp
• Locus ID: 6741
• UniProt ID: P05455
• Cytogenetics: 2q31.1
• Domains: RRM, LA
• Protein Families: Stem cell - Pluripotency, Transcription Factors
• Protein Pathways: Systemic lupus erythematosus
• Gene Summary: The protein encoded by this gene is involved in diverse aspects of RNA metabolism, including binding and protecting poly(U) termini of nascent RNA polymerase III transcripts from exonuclease digestion, processing 5' and 3' ends of pre-tRNA precursors, acting as an RNA chaperone, and binding viral RNAs associated with hepatitis C virus. Autoantibodies reacting with this protein are found in the sera of patients with Sjogren syndrome and systemic lupus erythematosus. Alternative promoter usage results in two different transcript variants which encode the same protein. [provided by RefSeq, Jun 2014]
  Transcript Variant: This variant (1) and variant 2 encode the same protein.