SLIT2 (NM_004787) Human Untagged Clone

CAT#: SC316293

SLIT2 (untagged)-Human slit homolog 2 (Drosophila) (SLIT2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SLIT2
• Synonyms: SLIL3; Slit-2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_004787 edited
  CAGACACTGCGCGGTTCCCTCGGAGCAGCAAGCTAAAGAAAGCCCCCAGTGCCGGCGAGG
  AAGGAGGCGGCGGGGAAAGATGCGCGGCGTTGGCTGGCAGATGCTGTCCCTGTCGCTGGG
  GTTAGTGCTGGCGATCCTGAACAAGGTGGCACCGCAGGCGTGCCCGGCGCAGTGCTCTTG
  CTCGGGCAGCACAGTGGACTGTCACGGGCTGGCGCTGCGCAGCGTGCCCAGGAATATCCC
  CCGCAACACCGAGAGACTGGATTTAAATGGAAATAACATCACAAGAATTACGAAGACAGA
  TTTTGCTGGTCTTAGACATCTAAGAGTTCTTCAGCTTATGGAGAATAAGATTAGCACCAT
  TGAAAGAGGAGCATTCCAGGATCTTAAAGAACTAGAGAGACTGCGTTTAAACAGAAATCA
  CCTTCAGCTGTTTCCTGAGTTGCTGTTTCTTGGGACTGCGAAGCTATACAGGCTTGATCT
  CAGTGAAAACCAAATTCAGGCAATCCCAAGGAAAGCTTTCCGTGGGGCAGTTGACATAAA
  AAATTTGCAACTGGATTACAACCAGATCAGCTGTATTGAAGATGGGGCATTCAGGGCTCT
  CCGGGACCTGGAAGTGCTCACTCTCAACAATAACAACATTACTAGACTTTCTGTGGCAAG
  TTTCAACCATATGCCTAAACTTAGGACTTTTCGACTGCATTCAAACAACCTGTATTGTGA
  CTGCCACCTGGCCTGGCTCTCCGACTGGCTTCGCCAAAGGCCTCGGGTTGGTCTGTACAC
  TCAGTGTATGGGCCCCTCCCACCTGAGAGGCCATAATGTAGCCGAGGTTCAAAAACGAGA
  ATTTGTCTGCAGTGTGGGTCACCAGTCATTTATGGCTCCTTCTTGTAGTGTTTTGCACTG
  CCCTGCCGCCTGTACCTGTAGCAACAATATCGTAGACTGTCGTGGGAAAGGTCTCACTGA
  GATCCCCACAAATCTTCCAGAGACCATCACAGAAATACGTTTGGAACAGAACACAATCAA
  AGTCATCCCTCCTGGAGCTTTCTCACCATATAAAAAGCTTAGACGAATTGACCTGAGCAA
  TAATCAGATCTCTGAACTTGCACCAGATGCTTTCCAAGGACTACGCTCTCTGAATTCACT
  TGTCCTCTATGGAAATAAAATCACAGAACTCCCCAAAAGTTTATTTGAAGGACTGTTTTC
  CTTACAGCTCCTATTATTGAATGCCAACAAGATAAACTGCCTTCGGGTAGATGCTTTTCA
  GGATCTCCACAACTTGAACCTTCTCTCCCTATATGACAACAAGCTTCAGACCATCGCCAA
  GGGGACCTTTTCACCTCTTCGGGCCATTCAAACTATGCATTTGGCCCAGAACCCCTTTAT
  TTGTGACTGCCATCTCAAGTGGCTAGCGGATTATCTCCATACCAACCCGATTGAGACCAG
  TGGTGCCCGTTGCACCAGCCCCCGCCGCCTGGCAAACAAAAGAATTGGACAGATCAAAAG
  CAAGAAATTCCGTTGTTCAGCTAAAGAACAGTATTTCATTCCAGGTACAGAAGATTATCG
  ATCAAAATTAAGTGGAGACTGCTTTGCGGATCTGGCTTGCCCTGAAAAGTGTCGCTGTGA
  AGGAACCACAGTAGATTGCTCTAATCAAAAGCTCAACAAAATCCCGGAGCACATTCCCCA
  GTACACTGCAGAGTTGCGTCTCAATAATAATGAATTTACCGTGTTGGAAGCCACAGGAAT
  CTTTAAGAAACTTCCTCAATTACGTAAAATAAACTTTAGCAACAATAAGATCACAGATAT
  TGAGGAGGGAGCATTTGAAGGAGCATCTGGTGTAAATGAAATACTTCTTACGAGTAATCG
  TTTGGAAAATGTGCAGCATAAGATGTTCAAGGGATTGGAAAGCCTCAAAACTTTGATGTT
  GAGAAGCAATCGAATAACCTGTGTGGGGAATGACAGTTTCATAGGACTCAGTTCTGTGCG
  TTTGCTTTCTTTGTATGATAATCAAATTACTACAGTTGCACCAGGGGCATTTGATACTCT
  CCATTCTTTATCTACTCTAAACCTCTTGGCCAATCCTTTTAACTGTAACTGCTACCTGGC
  TTGGTTGGGAGAGTGGCTGAGAAAGAAGAGAATTGTCACGGGAAATCCTAGATGTCAAAA
  ACCATACTTCCTGAAAGAAATACCCATCCAGGATGTGGCCATTCAGGACTTCACTTGTGA
  TGACGGAAATGATGACAATAGTTGCTCCCCACTTTCTCGCTGTCCTACTGAATGTACTTG
  CTTGGATACAGTCGTCCGATGTAGCAACAAGGGTTTGAAGGTCTTGCCGAAAGGTATTCC
  AAGAGATGTCACAGAGTTGTATCTGGATGGAAACCAATTTACACTGGTTCCCAAGGAACT
  CTCCAACTACAAACATTTAACACTTATAGACTTAAGTAACAACAGAATAAGCACGCTTTC
  TAATCAGAGCTTCAGCAACATGACCCAGCTCCTCACCTTAATTCTTAGTTACAACCGTCT
  GAGATGTATTCCTCCTCGCACCTTTGATGGATTAAAGTCTCTTCGATTACTTTCTCTACA
  TGGAAATGACATTTCTGTTGTGCCTGAAGGTGCTTTCAATGATCTTTCTGCATTATCACA
  TCTAGCAATTGGAGCCAACCCTCTTTACTGTGATTGTAACATGCAGTGGTTATCCGACTG
  GGTGAAGTCGGAATATAAGGAGCCTGGAATTGCTCGTTGTGCTGGTCCTGGAGAAATGGC
  AGATAAACTTTTACTCACAACTCCCTCCAAAAAATTTACCTGTCAAGGTCCTGTGGATGT
  CAATATTCTAGCTAAGTGTAACCCCTGCCTATCAAATCCGTGTAAAAATGATGGCACATG
  TAATAGTGATCCAGTTGACTTTTACCGATGCACCTGTCCATATGGTTTCAAGGGGCAGGA
  CTGTGATGTCCCAATTCATGCCTGCATCAGTAACCCATGTAAACATGGAGGAACTTGCCA
  CTTAAAGGAAGGAGAAGAAGATGGATTCTGGTGTATTTGTGCTGATGGATTTGAAGGAGA
  AAATTGTGAAGTCAACGTTGATGATTGTGAAGATAATGACTGTGAAAATAATTCTACATG
  TGTCGATGGCATTAATAACTACACATGCCTTTGCCCACCTGAGTATACAGGTGAGTTGTG
  TGAGGAGAAGCTGGACTTCTGTGCCCAGGACCTGAACCCCTGCCAGCACGATTCAAAGTG
  CATCCTAACTCCAAAGGGATTCAAATGTGACTGCACACCAGGGTACGTAGGTGAACACTG
  CGACATCGATTTTGACGACTGCCAAGACAACAAGTGTAAAAACGGAGCCCACTGCACAGA
  TGCAGTGAACGGCTATACGTGCATATGCCCCGAAGGTTACAGTGGCTTGTTCTGTGAGTT
  TTCTCCACCCATGGTCCTCCCTCGTACCAGCCCCTGTGATAATTTTGATTGTCAGAATGG
  AGCTCAGTGTATCGTCAGAATAAATGAGCCAATATGTCAGTGTTTGCCTGGCTATCAGGG
  AGAAAAGTGTGAAAAATTGGTTAGTGTGAATTTTATAAACAAAGAGTCTTATCTTCAGAT
  TCCTTCAGCCAAGGTTCGGCCTCAGACGAACATAACACTTCAGATTGCCACAGATGAAGA
  CAGCGGAATCCTCCTGTATAAGGGTGACAAAGACCATATCGCGGTAGAACTCTATCGGGG
  GCGTGTTCGTGCCAGCTATGACACCGGCTCTCATCCAGCTTCTGCCATTTACAGTGTGGA
  GACAATCAATGATGGAAACTTCCACATTGTGGAACTACTTGCCTTGGATCAGAGTCTCTC
  TTTGTCCGTGGATGGTGGGAACCCCAAAATCATCACTAACTTGTCAAAGCAGTCCACTCT
  GAATTTTGACTCTCCACTCTATGTAGGAGGCATGCCAGGGAAGAGTAACGTGGCATCTCT
  GCGCCAGGCCCCTGGGCAGAACGGAACCAGCTTCCACGGCTGCATCCGGAACCTTTACAT
  CAACAGTGAGCTGCAGGACTTCCAGAAGGTGCCGATGCAAACAGGCATTTTGCCTGGCTG
  TGAGCCATGCCACAAGAAGGTGTGTGCCCATGGCACATGCCAGCCCAGCAGCCAGGCAGG
  CTTCACCTGCGAGTGCCAGGAAGGATGGATGGGGCCCCTCTGTGACCAACGGACCAATGA
  CCCTTGCCTTGGAAATAAATGCGTACATGGCACCTGCTTGCCCATCAATGCGTTCTCCTA
  CAGCTGTAAGTGCTTGGAGGGCCATGGAGGTGTCCTCTGTGATGAAGAGGAGGATCTGTT
  TAACCCATGCCAGGCGATCAAGTGCAAGCAYGGGAAGTGCAGGCTTTCAGGTCTGGGGCA
  GCCCTACTGTGAATGCAGCAGTGGATACACGGGGGACAGCTGTGATCGAGAAATCTCTTG
  TCGAGGGGAAAGGATAAGAGATTATTACCAAAAGCAGCAGGGCTATGCTGCTTGCCAAAC
  AACCAAGAAGGTGTCCCGATTAGAGTGCAGAGGTGGGTGTGCAGGAGGGCAGTGCTGTGG
  ACCGCTGAGGAGCAAGCGGCGGAAATACTCTTTCGAATGCACTGACGGCTCCTCCTTTGT
  GGACGAGGTTGAGAAAGTGGTGAAGTGCGGCTGTACGAGGTGTGTGTCCTAAACACACTC
  CCGGCAGCTCTGTCTTT
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004787 unedited
  GTACCCTCTGTATACGACTCCTATAGGGCGGCCGCCAGTGTGATGGATATCTGCAGAATT
  CGCCCTTCAGACACTGCGCGGTTCCCTCGGAGCAGCAAGCTAAAGAAAGCCCCCAGTGCC
  GGCGAGGAAGGAGGCGGCGGGGAAAGATGCGCGGCGTTGGCTGGCAGATGCTGTCCCTGT
  CGCTGGGGTTAGTGCTGGCGATCCTGAACAAGGTGGCACCGCAGGCGTGCCCGGCGCAGT
  GCTCTTGCTCGGGCAGCACAGTGGACTGTCACGGGCTGGCGCTGCGCAGCGTGCCCAGGA
  ATATCCCCCGCAACACCGAGAGACTGGATTTAAATGGAAATAACATCACAAGAATTACGA
  AGACAGATTTTGCTGGTCTTAGACATCTAAGAGTTCTTCAGCTTATGGAGAATAAGATTA
  GCACCATTGAAAGAGGAGCATTCCAGGATCTTAAAGAACTAGAGAGACTGCGTTTAAACA
  GAAATCACCTTCAGCTGTTTCCTGAGTTGCTGTTTCTTGGGACTGCGAAGCTATACAGGC
  TTGATCTCAGTGAAAACCAAATTCAGGCAATCCCAAGGAAAGCTTTCCGTGGGGCAGTTG
  ACATAAAAAATTTGCAACTGGATTACAACCAGATCAGCTGTATTGAAGATGGGGCATTCA
  GGGCTCTCCGGGACCTGGAAGTGCTCACTCTCAACAATAACAACATTACTAGACTTTCTG
  TGGCAAGTTTCAACCATATGCCTAAACTTAGACTTTTCGACTGCATTCAAACAACCTGTA
  TTGTGACTGCCACCTGGCCCTGCTCTCCGACTGGCTTCGCCAAAGGCCTCGGGTTGGTCT
  GTACACTCAGTGTATGGGCCCCTCCCACCTGAGAGCCATAATGTAGCCGAGTTCAAAAAC
  GAGAATTTGTTCTGCAGTGGTCACCAGTCATTTATGGCT
  
• Restriction Sites: Please inquire
• ACCN: NM_004787
• Insert Size: 4700 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: The ORF of this clone has been fully sequenced and found to be a perfect match to NM_004787.1.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004787.1, NP_004778.1
• RefSeq Size: 4950 bp
• RefSeq ORF: 4590 bp
• Locus ID: 9353
• UniProt ID: O94813
• Cytogenetics: 4p15.31
• Domains: LRRNT, LRRCT, LRR, LamG, EGF_CA, LRR_TYP, CT, EGF, EGF, LRR_PS
• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Axon guidance
• Gene Summary: This gene encodes a member of the slit family of secreted glycoproteins, which are ligands for the Robo family of immunoglobulin receptors. Slit proteins play highly conserved roles in axon guidance and neuronal migration and may also have functions during other cell migration processes including leukocyte migration. Members of the slit family are characterized by an N-terminal signal peptide, four leucine-rich repeats, nine epidermal growth factor repeats, and a C-terminal cysteine knot. Proteolytic processing of this protein gives rise to an N-terminal fragment that contains the four leucine-rich repeats and five epidermal growth factor repeats and a C-terminal fragment that contains four epidermal growth factor repeats and the cysteine knot. Both full length and cleaved proteins are secreted extracellularly and can function in axon repulsion as well as other specific processes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.