ST18 (NM_014682) Human Untagged Clone

CAT#: SC315865

ST18 (untagged)-Human suppression of tumorigenicity 18 (breast carcinoma) (zinc finger protein) (ST18)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ST18
• Synonyms: NZF-3; NZF3; ZC2H2C3; ZC2HC10; ZNF387
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC315865 representing NM_014682.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGATGCAGAGGCTGAAGATAAAACGCTGCGTACTCGCTCTAAAGGAACCGAGGTGCCAATGGATTCA
  CTAATCCAGGAGCTCAGTGTTGCCTATGATTGCTCCATGGCAAAGAAGAGAACAGCTGAAGATCAGGCT
  TTGGGGGTTCCAGTCAACAAAAGGAAATCCCTGCTAATGAAGCCCCGACACTACAGCCCAAAAGCAGAC
  TGCCAAGAAGACCGCAGTGACAGGACAGAGGACGATGGCCCCTTGGAAACACATGGTCACTCTACCGCA
  GAGGAAATCATGATAAAACCTATGGATGAAAGTCTTCTTTCAACTGCACAAGAAAACTCCAGTAGGAAG
  GAAGACAGATACTCTTGTTATCAAGAGCTCATGGTCAAGTCTTTAATGCACTTGGGGAAATTTGAAAAA
  AATGTATCTGTTCAGACTGTAAGTGAAAATTTAAATGACAGTGGCATCCAGTCTTTAAAAGCAGAGAGC
  GATGAAGCAGACGAGTGCTTTCTGATTCATTCTGATGATGGAAGAGACAAGATTGATGATTCTCAGCCA
  CCCTTCTGCTCCTCTGATGACAATGAAAGTAACTCTGAAAGTGCAGAAAATGGCTGGGACAGTGGCTCC
  AACTTCTCAGAAGAAACCAAACCACCTAGAGTCCCAAAGTATGTTTTAACAGATCATAAAAAAGACCTA
  TTGGAAGTTCCTGAAATAAAAACTGAAGGTGACAAATTTATCCCTTGTGAGAACAGGTGTGATTCTGAA
  ACAGAAAGGAAAGACCCGCAGAATGCTCTCGCAGAACCCCTGGATGGCAATGCCCAGCCCTCATTCCCT
  GACGTTGAGGAGGAAGATAGCGAGAGCCTGGCAGTAATGACGGAAGAGGGTAGTGACCTGGAAAAGGCC
  AAGGGGAATTTAAGTTTGCTGGAGCAGGCAATTGCTCTGCAGGCTGAGCGAGGTTGTGTTTTCCATAAC
  ACCTACAAAGAGCTGGATAGGTTCCTGCTGGAGCACCTAGCAGGGGAAAGGAGGCAAACCAAAGTTATC
  GACATGGGTGGAAGACAAATCTTTAACAATAAACATTCACCAAGGCCTGAAAAGAGGGAGACCAAGTGC
  CCGATCCCTGGATGTGATGGCACGGGACACGTGACAGGGCTCTACCCGCACCACCGCAGCCTTTCGGGG
  TGCCCCCACAAAGTGCGGGTTCCCCTGGAAATTCTTGCCATGCATGAAAATGTGCTCAAGTGTCCCACG
  CCGGGATGCACAGGAAGGGGTCATGTGAACAGCAACCGCAACACCCACAGGAGTCTTTCTGGTTGTCCA
  ATTGCTGCAGCTGAAAAATTGGCAATGTCCCAGGATAAAAATCAGCTTGATTCTCCCCAAACTGGGCAG
  TGTCCTGACCAGGCCCACAGGACAAGTTTGGTGAAGCAAATTGAATTCAATTTCCCGTCACAAGCCATC
  ACCTCTCCCAGAGCCACAGTGTCAAAAGAACAAGAGAAGTTTGGAAAAGTACCATTTGATTATGCCAGT
  TTTGATGCCCAAGTTTTCGGTAAACGCCCTCTCATACAAACAGTGCAAGGACGAAAAACACCACCATTT
  CCTGAATCAAAGCATTTTCCAAATCCAGTGAAATTTCCTAATCGACTGCCTAGTGCAGGCGCCCACACC
  CAGAGCCCTGGCCGTGCCAGCTCTTATAGCTACGGTCAATGTAGTGAAGACACCCACATAGCAGCAGCT
  GCTGCCATCCTGAACCTTTCCACCCGCTGCAGGGAAGCCACAGACATCCTCTCCAACAAGCCACAGAGT
  CTGCATGCCAAGGGAGCCGAAATAGAAGTGGATGAAAATGGCACATTGGACTTAAGCATGAAAAAAAAT
  CGAATCCTGGACAAGTCTGCACCCCTAACTTCCTCTAACACTTCTATTCCAACTCCTTCCTCTTCCCCA
  TTCAAAACAAGCAGCATTCTGGTCAATGCAGCATTCTATCAGGCTCTTTGTGACCAAGAGGGCTGGGAC
  ACTCCTATCAACTATAGCAAAACTCACGGGAAGACAGAGGAGGAGAAAGAGAAAGACCCAGTGAGCTCT
  CTAGAAAATTTAGAGGAAAAAAAGTTTCCTGGAGAGGCCTCTATACCAAGCCCTAAACCCAAGCTTCAT
  GCAAGAGATCTCAAAAAGGAACTAATCACCTGTCCAACACCAGGATGTGATGGAAGTGGCCACGTGACA
  GGAAACTATGCATCTCATCGCAGTGTTTCTGGATGTCCTTTAGCAGATAAGACTCTAAAATCCCTCATG
  GCTGCCAACTCTCAGGAGCTTAAGTGTCCAACCCCAGGCTGCGATGGCTCGGGGCACGTGACTGGAAAC
  TATGCTTCCCACAGAAGCTTGTCCGGATGCCCTCGTGCAAGGAAAGGTGGTGTCAAAATGACCCCTACC
  AAGGAAGAAAAAGAAGACCCTGAACTGAAATGTCCTGTGATAGGGTGTGATGGCCAAGGTCACATATCA
  GGTAAATACACATCACACCGCACAGCTTCTGGCTGTCCTCTGGCTGCCAAGAGACAGAAGGAGAATCCT
  CTCAATGGAGCCTCCCTCTCCTGGAAACTGAACAAACAAGAGCTACCACATTGTCCCTTGCCAGGCTGC
  AATGGGCTGGGCCATGTAAATAATGTTTTTGTCACCCACCGAAGCTTATCTGGATGTCCTCTCAATGCA
  CAAGTTATCAAAAAGGGCAAGGTTTCTGAAGAACTCATGACCATCAAGCTCAAAGCAACTGGGGGAATA
  GAGAGTGATGAAGAAATTAGGCATTTGGATGAAGAAATAAAGGAACTGAATGAATCCAACCTTAAAATT
  GAAGCAGATATGATGAAACTTCAGACCCAGATCACATCTATGGAGAGCAACTTAAAGACGATAGAGGAG
  GAGAACAAACTCATAGAACAGAACAATGAAAGTCTGCTGAAAGAGCTGGCAGGTCTAAGCCAAGCTCTC
  ATTTCAAGCCTTGCTGACATCCAGCTTCCACAGATGGGACCTATCAGTGAGCAGAATTTTGAAGCATAT
  GTAAATACACTCACAGATATGTACAGCAATCTGGAACGGGACTATTCCCCGGAATGCAAAGCTCTACTG
  GAAAGTATCAAACAGGCAGTGAAGGGTATCCATGTGTAG
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_014682
• Insert Size: 3144 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014682.2
• RefSeq Size: 6330 bp
• RefSeq ORF: 3144 bp
• Locus ID: 9705
• UniProt ID: O60284
• Cytogenetics: 8q11.23
• Domains: zf-C2HC
• Protein Families: Transcription Factors
• MW: 115.2 kDa
• Gene Summary: Repressor that binds to DNA sequences containing a bipartite element consisting of a direct repeat of the sequence 5'-AAAGTTT-3' separated by 2-9 nucleotides. Represses basal transcription activity from target promoters (By similarity). Inhibits colony formation in cultured breast cancer cells.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes isoform a. Variants 1-33 all encode isoform a.